Cyanophycin synthase (L-arginine-adding)

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Cyanophycin synthase (L-arginine-adding) (EC 6.3.2.30, CphA, CphA1, CphA2, cyanophycin synthetase, multi-L-arginyl-poly-L-aspartate synthase) is an enzyme with systematic name cyanophycin:L-arginine ligase (ADP-forming).^[1]^[2]^[3]^[4]^[5]^[6] This enzyme catalyses the following chemical reaction

ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg

\rightleftharpoons

ADP + phosphate + [L-Asp(4-L-Arg)]n+1

This enzyme requires Mg²⁺ for activity.

References

↑ Aboulmagd, E., Oppermann-Sanio, F.B. and Steinbüchel, A. (2000). "Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308". Arch. Microbiol. 174 (5): 297–306. doi:10.1007/s002030000206. PMID 11131019.
↑ Aboulmagd, E., Oppermann-Sanio, F.B. and Steinbüchel, A. (2001). "Purification of Synechocystis sp. strain PCC6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity". Appl. Environ. Microbiol. 67: 2176–2182. doi:10.1128/AEM.67.5.2176-2182.2001. PMID 11319097.
↑ Allen, M.M., Hutchison, F. and Weathers, P.J. (1980). "Cyanophycin granule polypeptide formation and degradation in the cyanobacterium Aphanocapsa 6308". J. Bacteriol. 141 (2): 687–693. PMID 6767688.
↑ Berg, H., Ziegler, K., Piotukh, K., Baier, K., Lockau, W. and Volkmer-Engert, R. (2000). "Biosynthesis of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartic acid (cyanophycin): mechanism of the cyanophycin synthetase reaction studied with synthetic primers". Eur. J. Biochem. 267 (17): 5561–5570. doi:10.1046/j.1432-1327.2000.01622.x. PMID 10951215.
↑ Ziegler, K., Deutzmann, R. and Lockau, W. (2002). "Cyanophycin synthetase-like enzymes of non-cyanobacterial eubacteria: characterization of the polymer produced by a recombinant synthetase of Desulfitobacterium hafniense". Z. Naturforsch. C 57 (5-6): 522–529. doi:10.1515/znc-2002-5-621. PMID 12132696.
↑ Ziegler, K., Diener, A., Herpin, C., Richter, R., Deutzmann, R. and Lockau, W. (1998). "Molecular characterization of cyanophycin synthetase, the enzyme catalyzing the biosynthesis of the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartate (cyanophycin)". Eur. J. Biochem. 254: 154–159. doi:10.1046/j.1432-1327.1998.2540154.x. PMID 9652408.

External links

Cyanophycin synthase (L-arginine-adding) at the US National Library of Medicine Medical Subject Headings (MeSH)

Enzymes: CO CS and CN ligases (EC 6.1-6.3)

6.1: Carbon-Oxygen	Aminoacyl tRNA synthetase Alanine Arginine Asparagine Aspartate Cysteine D-alanine—poly(phosphoribitol) ligase Glutamate Glutamine Glycine Histidine Isoleucine Leucine Lysine Methionine Phenylalanine Proline Serine Threonine Tryptophan Tyrosine Valine

6.2: Carbon-Sulfur	Succinyl coenzyme A synthetase Acetyl—CoA synthetase Long-chain-fatty-acid—CoA ligase

6.3: Carbon-Nitrogen	Glutamine synthetase Ubiquitin ligase Cullin Von Hippel–Lindau tumor suppressor UBE3A Mdm2 Anaphase-promoting complex UBR1 Glutathione synthetase CTP synthetase Adenylosuccinate synthase Argininosuccinate synthase Holocarboxylase synthetase GMP synthase Asparagine synthetase Carbamoyl phosphate synthetase I II Glutamate–cysteine ligase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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