DMAP1

DNA methyltransferase 1 associated protein 1
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3HM5​, 4IEJ​
Identifiers
Symbols	DMAP1 ; DNMAP1; DNMTAP1; EAF2; MEAF2; SWC4
External IDs	OMIM: 605077 MGI: 1913483 HomoloGene: 41311 GeneCards: DMAP1 Gene
Gene ontology Molecular function • RNA polymerase II repressing transcription factor binding • transcription corepressor activity • protein binding Cellular component • nucleus • nucleoplasm • replication fork • cytoplasm • NuA4 histone acetyltransferase complex Biological process • negative regulation of transcription from RNA polymerase II promoter • DNA repair • DNA methylation • chromatin organization • chromatin remodeling • transcription, DNA-templated • regulation of growth • positive regulation of transcription factor import into nucleus • histone H4 acetylation • histone H2A acetylation • response to ethanol • negative regulation of transcription, DNA-templated Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	55929	66233
Ensembl	ENSG00000178028	ENSMUSG00000009640
UniProt	Q9NPF5	Q9JI44
RefSeq (mRNA)	NM_001034023	NM_023178
RefSeq (protein)	NP_001029195	NP_075667
Location (UCSC)	Chr 1: 44.21 – 44.22 Mb	Chr 4: 117.67 – 117.68 Mb
PubMed search

DNA methyltransferase 1-associated protein 1 is an enzyme that in humans is encoded by the DMAP1 gene.^[1][2][3]

Function

This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described.^[3]

Interactions

DMAP1 has been shown to interact with:

• C19orf2,^[4]
• DNMT1,^[5]
• ING1,^[6] and
• RGS6^[7]

References

1. Rountree MR, Bachman KE, Baylin SB (Aug 2000). "DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci". Nat Genet 25 (3): 269–77. doi:10.1038/77023. PMID 10888872.
2. Nagase T, Kikuno R, Ishikawa KI, Hirosawa M, Ohara O (Apr 2000). "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro". DNA Res 7 (1): 65–73. doi:10.1093/dnares/7.1.65. PMID 10718198.
3. "Entrez Gene: DMAP1 DNA methyltransferase 1 associated protein 1".
4. Delgermaa L, Hayashi N, Dorjsuren D, Nomura T, Thuy le TT, Murakami S (Oct 2004). "Subcellular localization of RPB5-mediating protein and its putative functional partner". Mol. Cell. Biol. 24 (19): 8556–66. doi:10.1128/MCB.24.19.8556-8566.2004. PMC 516735. PMID 15367675.
5. Rountree MR, Bachman KE, Baylin SB (Jul 2000). "DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci". Nat. Genet. 25 (3): 269–77. doi:10.1038/77023. PMID 10888872.
6. Xin H, Yoon HG, Singh PB, Wong J, Qin J (Mar 2004). "Components of a pathway maintaining histone modification and heterochromatin protein 1 binding at the pericentric heterochromatin in Mammalian cells". J. Biol. Chem. 279 (10): 9539–46. doi:10.1074/jbc.M311587200. PMID 14665632.
7. Liu Z, Fisher RA (Apr 2004). "RGS6 interacts with DMAP1 and DNMT1 and inhibits DMAP1 transcriptional repressor activity". J. Biol. Chem. 279 (14): 14120–8. doi:10.1074/jbc.M309547200. PMID 14734556.

Further reading

• Doyon Y, Côté J (2004). "The highly conserved and multifunctional NuA4 HAT complex.". Curr. Opin. Genet. Dev. 14 (2): 147–54. doi:10.1016/j.gde.2004.02.009. PMID 15196461.
• Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination.". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Wiemann S, Weil B, Wellenreuther R; et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Yang CS, Weiner H (2002). "Yeast two-hybrid screening identifies binding partners of human Tom34 that have ATPase activity and form a complex with Tom34 in the cytosol.". Arch. Biochem. Biophys. 400 (1): 105–10. doi:10.1006/abbi.2002.2778. PMID 11913976.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Cai Y, Jin J, Tomomori-Sato C; et al. (2003). "Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex.". J. Biol. Chem. 278 (44): 42733–6. doi:10.1074/jbc.C300389200. PMID 12963728.
• Xin H, Yoon HG, Singh PB; et al. (2004). "Components of a pathway maintaining histone modification and heterochromatin protein 1 binding at the pericentric heterochromatin in Mammalian cells.". J. Biol. Chem. 279 (10): 9539–46. doi:10.1074/jbc.M311587200. PMID 14665632.
• Ota T, Suzuki Y, Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Liu Z, Fisher RA (2004). "RGS6 interacts with DMAP1 and DNMT1 and inhibits DMAP1 transcriptional repressor activity.". J. Biol. Chem. 279 (14): 14120–8. doi:10.1074/jbc.M309547200. PMID 14734556.
• Doyon Y, Selleck W, Lane WS; et al. (2004). "Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans.". Mol. Cell. Biol. 24 (5): 1884–96. doi:10.1128/MCB.24.5.1884-1896.2004. PMC 350560. PMID 14966270.
• Muromoto R, Sugiyama K, Takachi A; et al. (2004). "Physical and functional interactions between Daxx and DNA methyltransferase 1-associated protein, DMAP1.". J. Immunol. 172 (5): 2985–93. doi:10.4049/jimmunol.172.5.2985. PMID 14978102.
• Muromoto R, Sugiyama K, Yamamoto T; et al. (2004). "Physical and functional interactions between Daxx and TSG101.". Biochem. Biophys. Res. Commun. 316 (3): 827–33. doi:10.1016/j.bbrc.2004.02.126. PMID 15033475.
• Ballif BA, Villén J, Beausoleil SA; et al. (2005). "Phosphoproteomic analysis of the developing mouse brain.". Mol. Cell Proteomics 3 (11): 1093–101. doi:10.1074/mcp.M400085-MCP200. PMID 15345747.
• Delgermaa L, Hayashi N, Dorjsuren D; et al. (2004). "Subcellular localization of RPB5-mediating protein and its putative functional partner.". Mol. Cell. Biol. 24 (19): 8556–66. doi:10.1128/MCB.24.19.8556-8566.2004. PMC 516735. PMID 15367675.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Wiemann S, Arlt D, Huber W; et al. (2004). "From ORFeome to biology: a functional genomics pipeline.". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Cai Y, Jin J, Florens L; et al. (2005). "The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes.". J. Biol. Chem. 280 (14): 13665–70. doi:10.1074/jbc.M500001200. PMID 15647280.