DNA oxidative demethylase

DNA oxidative demethylase
Identifiers
EC number	1.14.11.33
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

DNA oxidative demethylase (EC 1.14.11.33, alkylated DNA repair protein, alpha-ketoglutarate-dependent dioxygenase ABH1, alkB (gene)) is an enzyme with systematic name methyl DNA-base, 2-oxoglutarate:oxygen oxidoreductase (formaldehyde-forming).^[1][2][3] This enzyme catalyses the following chemical reaction

DNA-base-CH₃ + 2-oxoglutarate + O₂ $\rightleftharpoons$ DNA-base + formaldehyde + succinate + CO₂

DNA oxidative demethylase contains iron; activity is somewhat stimulated by ascorbate.

References

1. Falnes, P.O., Johansen, R.F. and Seeberg, E. (2002). "AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli". Nature 419 (6903): 178–182. Bibcode:2002Natur.419..178F. doi:10.1038/nature01048. PMID 12226668.
2. Yi, C., Yang, C.G. and He, C. (2009). "A non-heme iron-mediated chemical demethylation in DNA and RNA". Acc. Chem. Res. 42 (4): 519–529. doi:10.1021/ar800178j. PMC 2920458. PMID 19852088.
3. Yi, C., Jia, G., Hou, G., Dai, Q., Zhang, W., Zheng, G., Jian, X., Yang, C.G., Cui, Q. and He, C. (2010). "Iron-catalysed oxidation intermediates captured in a DNA repair dioxygenase". Nature 468 (7321): 330–333. Bibcode:2010Natur.468..330Y. doi:10.1038/nature09497. PMC 3058853. PMID 21068844.

External links

• DNA oxidative demethylase at the US National Library of Medicine Medical Subject Headings (MeSH)