DYNLL1

Dynein, light chain, LC8-type 1
PDB rendering based on 1cmi.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1CMI​, 3ZKE​, 3ZKF​
Identifiers
Symbols	DYNLL1 ; DLC1; DLC8; DNCL1; DNCLC1; LC8; LC8a; PIN; hdlc1
External IDs	OMIM: 601562 MGI: 1861457 HomoloGene: 133063 GeneCards: DYNLL1 Gene
Gene ontology Molecular function • motor activity • protein binding • protein C-terminus binding • protein domain specific binding Cellular component • kinetochore • nucleus • cytoplasm • mitochondrion • centrosome • cytosol • cytoplasmic dynein complex • microtubule • plasma membrane • COP9 signalosome • membrane • extracellular exosome • primary cilium • mitotic spindle • ciliary tip Biological process • G2/M transition of mitotic cell cycle • mitotic cell cycle • transcription, DNA-templated • regulation of transcription, DNA-templated • apoptotic process • organelle organization • female gamete generation • anatomical structure morphogenesis • programmed cell death • viral process • antigen processing and presentation of exogenous peptide antigen via MHC class II • substantia nigra development • actin cytoskeleton organization • intraciliary retrograde transport • negative regulation of phosphorylation • cilium morphogenesis • intrinsic apoptotic signaling pathway • positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	8655	56455
Ensembl	ENSG00000088986	ENSMUSG00000009013
UniProt	P63167	P63168
RefSeq (mRNA)	NM_001037494	NM_019682
RefSeq (protein)	NP_001032583	NP_062656
Location (UCSC)	Chr 12: 120.47 – 120.5 Mb	Chr 5: 115.3 – 115.3 Mb
PubMed search

Dynein light chain 1, cytoplasmic is a protein that in humans is encoded by the DYNLL1 gene.^[1][2][3][4]

Function

Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized.^[4]

Interactions

DYNLL1 has been shown to interact with:

• BCL2L11,^[5][6]
• DLG4^[7]
• DLGAP1,^[7]
• DYNC1I1,^[8]
• IκBα,^[9]
• MYO5A,^[7]
• NRF1,^[10]
• PAK1,^[6] and
• TP53BP1.^[11]

References

1. Dick T, Ray K, Salz HK, Chia W (May 1996). "Cytoplasmic dynein (ddlc1) mutations cause morphogenetic defects and apoptotic cell death in Drosophila melanogaster". Molecular and Cellular Biology 16 (5): 1966–77. doi:10.1128/mcb.16.5.1966. PMC 231184. PMID 8628263.
2. Jaffrey SR, Snyder SH (Nov 1996). "PIN: an associated protein inhibitor of neuronal nitric oxide synthase". Science 274 (5288): 774–7. doi:10.1126/science.274.5288.774. PMID 8864115.
3. Pfister KK, Fisher EM, Gibbons IR, Hays TS, Holzbaur EL, McIntosh JR, Porter ME, Schroer TA, Vaughan KT, Witman GB, King SM, Vallee RB (Nov 2005). "Cytoplasmic dynein nomenclature". The Journal of Cell Biology 171 (3): 411–3. doi:10.1083/jcb.200508078. PMC 2171247. PMID 16260502.
4. "Entrez Gene: DYNLL1 dynein, light chain, LC8-type 1".
5. Day CL, Puthalakath H, Skea G, Strasser A, Barsukov I, Lian LY, Huang DC, Hinds MG (Feb 2004). "Localization of dynein light chains 1 and 2 and their pro-apoptotic ligands". The Biochemical Journal 377 (Pt 3): 597–605. doi:10.1042/BJ20031251. PMC 1223895. PMID 14561217.
6. Vadlamudi RK, Bagheri-Yarmand R, Yang Z, Balasenthil S, Nguyen D, Sahin AA, den Hollander P, Kumar R (Jun 2004). "Dynein light chain 1, a p21-activated kinase 1-interacting substrate, promotes cancerous phenotypes". Cancer Cell 5 (6): 575–85. doi:10.1016/j.ccr.2004.05.022. PMID 15193260.
7. Naisbitt S, Valtschanoff J, Allison DW, Sala C, Kim E, Craig AM, Weinberg RJ, Sheng M (Jun 2000). "Interaction of the postsynaptic density-95/guanylate kinase domain-associated protein complex with a light chain of myosin-V and dynein". The Journal of Neuroscience 20 (12): 4524–34. PMID 10844022.
8. Diefenbach RJ, Diefenbach E, Douglas MW, Cunningham AL (Dec 2002). "The heavy chain of conventional kinesin interacts with the SNARE proteins SNAP25 and SNAP23". Biochemistry 41 (50): 14906–15. doi:10.1021/bi026417u. PMID 12475239.
9. Crépieux P, Kwon H, Leclerc N, Spencer W, Richard S, Lin R, Hiscott J (Dec 1997). "I kappaB alpha physically interacts with a cytoskeleton-associated protein through its signal response domain". Molecular and Cellular Biology 17 (12): 7375–85. PMC 232593. PMID 9372968.
10. Herzig RP, Andersson U, Scarpulla RC (Dec 2000). "Dynein light chain interacts with NRF-1 and EWG, structurally and functionally related transcription factors from humans and drosophila". Journal of Cell Science 113 (23): 4263–73. PMID 11069771.
11. Lo KW, Kan HM, Chan LN, Xu WG, Wang KP, Wu Z, Sheng M, Zhang M (Mar 2005). "The 8-kDa dynein light chain binds to p53-binding protein 1 and mediates DNA damage-induced p53 nuclear accumulation". The Journal of Biological Chemistry 280 (9): 8172–9. doi:10.1074/jbc.M411408200. PMID 15611139.

Further reading

• Robertson NG, Khetarpal U, Gutiérrez-Espeleta GA, Bieber FR, Morton CC (Sep 1994). "Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening". Genomics 23 (1): 42–50. doi:10.1006/geno.1994.1457. PMID 7829101.
• Crépieux P, Kwon H, Leclerc N, Spencer W, Richard S, Lin R, Hiscott J (Dec 1997). "I kappaB alpha physically interacts with a cytoskeleton-associated protein through its signal response domain". Molecular and Cellular Biology 17 (12): 7375–85. PMC 232593. PMID 9372968.
• Tochio H, Ohki S, Zhang Q, Li M, Zhang M (Nov 1998). "Solution structure of a protein inhibitor of neuronal nitric oxide synthase". Nature Structural Biology 5 (11): 965–9. doi:10.1038/2940. PMID 9808041.
• Rodríguez-Crespo I, Straub W, Gavilanes F, Ortiz de Montellano PR (Nov 1998). "Binding of dynein light chain (PIN) to neuronal nitric oxide synthase in the absence of inhibition". Archives of Biochemistry and Biophysics 359 (2): 297–304. doi:10.1006/abbi.1998.0928. PMID 9808772.
• Fan JS, Zhang Q, Li M, Tochio H, Yamazaki T, Shimizu M, Zhang M (Dec 1998). "Protein inhibitor of neuronal nitric-oxide synthase, PIN, binds to a 17-amino acid residue fragment of the enzyme". The Journal of Biological Chemistry 273 (50): 33472–81. doi:10.1074/jbc.273.50.33472. PMID 9837926.
• Puthalakath H, Huang DC, O'Reilly LA, King SM, Strasser A (Mar 1999). "The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex". Molecular Cell 3 (3): 287–96. doi:10.1016/S1097-2765(00)80456-6. PMID 10198631.
• Liang J, Jaffrey SR, Guo W, Snyder SH, Clardy J (Aug 1999). "Structure of the PIN/LC8 dimer with a bound peptide". Nature Structural Biology 6 (8): 735–40. doi:10.1038/11501. PMID 10426949.
• Epstein E, Sela-Brown A, Ringel I, Kilav R, King SM, Benashski SE, Yisraeli JK, Silver J, Naveh-Many T (Feb 2000). "Dynein light chain binding to a 3'-untranslated sequence mediates parathyroid hormone mRNA association with microtubules". The Journal of Clinical Investigation 105 (4): 505–12. doi:10.1172/JCI8557. PMC 289163. PMID 10683380.
• Naisbitt S, Valtschanoff J, Allison DW, Sala C, Kim E, Craig AM, Weinberg RJ, Sheng M (Jun 2000). "Interaction of the postsynaptic density-95/guanylate kinase domain-associated protein complex with a light chain of myosin-V and dynein". The Journal of Neuroscience 20 (12): 4524–34. PMID 10844022.
• Jacob Y, Badrane H, Ceccaldi PE, Tordo N (Nov 2000). "Cytoplasmic dynein LC8 interacts with lyssavirus phosphoprotein". Journal of Virology 74 (21): 10217–22. doi:10.1128/JVI.74.21.10217-10222.2000. PMC 102062. PMID 11024152.
• Herzig RP, Andersson U, Scarpulla RC (Dec 2000). "Dynein light chain interacts with NRF-1 and EWG, structurally and functionally related transcription factors from humans and drosophila". Journal of Cell Science 113 (23): 4263–73. PMID 11069771.
• Haraguchi K, Satoh K, Yanai H, Hamada F, Kawabuchi M, Akiyama T (Nov 2000). "The hDLG-associated protein DAP interacts with dynein light chain and neuronal nitric oxide synthase". Genes to Cells 5 (11): 905–911. doi:10.1046/j.1365-2443.2000.00374.x. PMID 11122378.
• Lo KW, Naisbitt S, Fan JS, Sheng M, Zhang M (Apr 2001). "The 8-kDa dynein light chain binds to its targets via a conserved (K/R)XTQT motif". The Journal of Biological Chemistry 276 (17): 14059–66. doi:10.1074/jbc.M010320200. PMID 11148209.
• Yano H, Lee FS, Kong H, Chuang J, Arevalo J, Perez P, Sung C, Chao MV (Feb 2001). "Association of Trk neurotrophin receptors with components of the cytoplasmic dynein motor". The Journal of Neuroscience 21 (3): RC125. PMID 11157096.
• Fan J, Zhang Q, Tochio H, Li M, Zhang M (Feb 2001). "Structural basis of diverse sequence-dependent target recognition by the 8 kDa dynein light chain". Journal of Molecular Biology 306 (1): 97–108. doi:10.1006/jmbi.2000.4374. PMID 11178896.
• Bielli A, Thörnqvist PO, Hendrick AG, Finn R, Fitzgerald K, McCaffrey MW (Mar 2001). "The small GTPase Rab4A interacts with the central region of cytoplasmic dynein light intermediate chain-1". Biochemical and Biophysical Research Communications 281 (5): 1141–53. doi:10.1006/bbrc.2001.4468. PMID 11243854.
• Yu J, Yu L, Chen Z, Zheng L, Chen X, Wang X, Ren D, Zhao S (Mar 2002). "Protein inhibitor of neuronal nitric oxide synthase interacts with protein kinase A inhibitors". Brain Research. Molecular Brain Research 99 (2): 145–9. doi:10.1016/S0169-328X(02)00104-3. PMID 11978406.
• Fuhrmann JC, Kins S, Rostaing P, El Far O, Kirsch J, Sheng M, Triller A, Betz H, Kneussel M (Jul 2002). "Gephyrin interacts with Dynein light chains 1 and 2, components of motor protein complexes". The Journal of Neuroscience 22 (13): 5393–402. PMID 12097491.