Deoxycytidine kinase

PDB rendering based on 1p5z.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
1P5Z, 1P60, 1P61, 1P62, 2A2Z, 2A30, 2A7Q, 2NO0, 2NO1, 2NO6, 2NO7, 2NO9, 2NOA, 2QRN, 2QRO, 2ZI3, 2ZI4, 2ZI5, 2ZI6, 2ZI7, 2ZI9, 2ZIA, 3HP1, 3IPX, 3IPY, 3KFX, 3MJR, 3QEJ, 3QEN, 3QEO, 4JLJ, 4JLK, 4JLM, 4JLN, 4KCG, 4L5B, 4Q18, 4Q19, 4Q1A, 4Q1B, 4Q1C, 4Q1D, 4Q1E, 4Q1F

Identifiers

Symbols

DCK ; MGC117410; MGC138632

External IDs

OMIM: 125450 MGI: 102726 HomoloGene: 616 ChEMBL: 2447 GeneCards: DCK Gene

EC number

2.7.1.74

Gene ontology
Molecular function	• deoxycytidine kinase activity • ATP binding • drug binding • nucleoside kinase activity • protein homodimerization activity
Cellular component	• nucleus • cytosol
Biological process	• purine nucleobase metabolic process • pyrimidine nucleobase metabolic process • pyrimidine nucleotide metabolic process • deoxyribonucleoside monophosphate biosynthetic process • nucleotide biosynthetic process • phosphorylation • pyrimidine nucleoside salvage • purine-containing compound salvage • small molecule metabolic process • nucleobase-containing small molecule metabolic process
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 4:
70.99 – 71.03 Mb

Chr 5:
88.76 – 88.78 Mb

PubMed search

Deoxycytidine kinase, also known as DCK, is an enzyme which in humans is encoded by the DCK gene.^[1]

DCK transfers phosphate to deoxycytidine.

Function

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.^[1]

References

1 2 "Entrez Gene: DCK deoxycytidine kinase".

Hazra S, Szewczak A, Ort S, Konrad M, Lavie A (February 2011). "Post-translational phosphorylation of serine 74 of human deoxycytidine kinase favors the enzyme adopting the open conformation making it competent for nucleoside binding and release". Biochemistry 50 (14): 2870–80. doi:10.1021/bi2001032. PMC 3071448. PMID 21351740.
Hazra S, Konrad M, Lavie A (August 2010). "The sugar ring of the nucleoside is required for productive substrate positioning in the active site of human deoxycytidine kinase (dCK): implications for the development of dCK-activated acyclic guanine analogs". J Med Chem 53 (15): 5792–800. doi:10.1021/jm1005379. PMC 2936711. PMID 20684612.
Hazra S, Ort S, Konrad M, Lavie A (August 2010). "Structural and kinetic characterization of human deoxycytidine kinase variants able to phosphorylate 5-substituted deoxycytidine and thymidine analogs". Biochemistry 49 (31): 6748–90. doi:10.1021/bi100839e. PMC 2925221. PMID 20614893.
Hazra S, Sabini E, Ort S, Konrad M, Lavie A (January 2009). "Extending thymidine kinase activity to the catalytic repertoire of human deoxycytidine kinase". Biochemistry 48 (6): 1256–63. doi:10.1021/bi802062w. PMC 2701478. PMID 19159229.
Sabini E, Hazra S, Konrad M, Lavie A (July 2008). "Elucidation of Different Binding Modes of Purine Nucleosides to Human Deoxycytidine Kinase". J. Med. Chem. 51 (14): 4219–25. doi:10.1021/jm800134t. PMC 2636677. PMID 18570408.
Sabini E, Hazra S, Ort S, Konrad M, Lavie A (May 2008). "Structural basis for substrate promiscuity of dCK". J. Mol. Biol. 378 (3): 607–21. doi:10.1016/j.jmb.2008.02.061. PMC 2426910. PMID 18377927.
McSorley T, Ort S, Hazra S, Lavie A, Konrad M (March 2008). "Mimicking phosphorylation of Ser-74 on human deoxycytidine kinase selectively increases catalytic activity for dC and dC analogues". FEBS Lett. 582 (5): 720–4. doi:10.1016/j.febslet.2008.01.048. PMC 2636680. PMID 18258203.
Sabini E, Hazra S, Konrad M, Lavie A (June 2007). "The non-enantioselectivity property of human deoxycytidine kinase explained by structures of the enzyme in complex with l- and d-nucleosides". J. Med. Chem. 50 (13): 3004–14. doi:10.1021/jm0700215. PMC 2586175. PMID 17530837.
Sabini E, Hazra S, Konrad M, Burley SK, Lavie A (2007). "Structural basis for activation of the therapeutic l-nucleoside analogs 3TC and troxacitabine by human deoxycytidine kinase". Nucleic Acids Res. 35 (1): 186–92. doi:10.1093/nar/gkl1038. PMC 1802566. PMID 17158155.

Arnér ES, Eriksson S (1996). "Mammalian deoxyribonucleoside kinases". Pharmacol. Ther. 67 (2): 155–86. doi:10.1016/0163-7258(95)00015-9. PMID 7494863.
Chottiner EG, Shewach DS, Datta NS; et al. (1991). "Cloning and expression of human deoxycytidine kinase cDNA". Proc. Natl. Acad. Sci. U.S.A. 88 (4): 1531–5. doi:10.1073/pnas.88.4.1531. PMC 51053. PMID 1996353.
Eriksson S, Cederlund E, Bergman T; et al. (1991). "Characterization of human deoxycytidine kinase. Correlation with cDNA sequences". FEBS Lett. 280 (2): 363–6. doi:10.1016/0014-5793(91)80332-W. PMID 2013338.
Yamada Y, Goto H, Ogasawara N (1984). "Purine nucleoside kinases in human T- and B-lymphoblasts". Biochim. Biophys. Acta 761 (1): 34–40. doi:10.1016/0304-4165(83)90359-8. PMID 6315069.
Hurley MC, Palella TD, Fox IH (1984). "Human placental deoxyadenosine and deoxyguanosine phosphorylating activity". J. Biol. Chem. 258 (24): 15021–7. PMID 6317685.
Spasokoukotskaja T, Arnér ES, Brosjö O; et al. (1995). "Expression of deoxycytidine kinase and phosphorylation of 2-chlorodeoxyadenosine in human normal and tumour cells and tissues". Eur. J. Cancer 31A (2): 202–8. doi:10.1016/0959-8049(94)00435-8. PMID 7718326.
Stegmann AP, Honders MW, Bolk MW; et al. (1993). "Assignment of the human deoxycytidine kinase (DCK) gene to chromosome 4 band q13.3-q21.1". Genomics 17 (2): 528–9. doi:10.1006/geno.1993.1365. PMID 8406512.
Song JJ, Walker S, Chen E; et al. (1993). "Genomic structure and chromosomal localization of the human deoxycytidine kinase gene". Proc. Natl. Acad. Sci. U.S.A. 90 (2): 431–4. doi:10.1073/pnas.90.2.431. PMC 45676. PMID 8421671.
Johansson M, Brismar S, Karlsson A (1997). "Human deoxycytidine kinase is located in the cell nucleus". Proc. Natl. Acad. Sci. U.S.A. 94 (22): 11941–5. doi:10.1073/pnas.94.22.11941. PMC 23663. PMID 9342341.
Hatzis P, Al-Madhoon AS, Jüllig M; et al. (1998). "The intracellular localization of deoxycytidine kinase". J. Biol. Chem. 273 (46): 30239–43. doi:10.1074/jbc.273.46.30239. PMID 9804782.
Saada A, Shaag A, Mandel H; et al. (2001). "Mutant mitochondrial thymidine kinase in mitochondrial DNA depletion myopathy". Nat. Genet. 29 (3): 342–4. doi:10.1038/ng751. PMID 11687801.
Veuger MJ, Heemskerk MH, Honders MW; et al. (2002). "Functional role of alternatively spliced deoxycytidine kinase in sensitivity to cytarabine of acute myeloid leukemic cells". Blood 99 (4): 1373–80. doi:10.1182/blood.V99.4.1373. PMID 11830489.
Innoceta A, Galluzzi L, Ruzzo A; et al. (2002). "Molecular basis of 2',3'-dideoxycytidine-induced drug resistance in human cells". Mol. Cell. Biochem. 231 (1–2): 173–7. doi:10.1023/A:1014441209108. PMID 11952160.
Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Krawiec K, Kierdaszuk B, Shugar D (2003). "Inorganic tripolyphosphate (PPP(i)) as a phosphate donor for human deoxyribonucleoside kinases". Biochem. Biophys. Res. Commun. 301 (1): 192–7. doi:10.1016/S0006-291X(02)03007-3. PMID 12535661.
van der Wilt CL, Kroep JR, Loves WJ; et al. (2003). "Expression of deoxycytidine kinase in leukaemic cells compared with solid tumour cell lines, liver metastases and normal liver". Eur. J. Cancer 39 (5): 691–7. doi:10.1016/S0959-8049(02)00813-4. PMID 12628850.
Ge Y, Jensen TL, Matherly LH, Taub JW (2004). "Physical and functional interactions between USF and Sp1 proteins regulate human deoxycytidine kinase promoter activity". J. Biol. Chem. 278 (50): 49901–10. doi:10.1074/jbc.M305085200. PMID 14514691.
Usova E, Maltseva T, Földesi A; et al. (2005). "Human deoxycytidine kinase as a deoxyribonucleoside phosphorylase". J. Mol. Biol. 344 (5): 1347–58. doi:10.1016/j.jmb.2004.10.016. PMID 15561147.
Mani RS, Usova EV, Eriksson S, Cass CE (2005). "Fluorescence studies of substrate binding to human recombinant deoxycytidine kinase". Nucleosides Nucleotides Nucleic Acids 23 (8–9): 1343–6. doi:10.1081/NCN-200027609. PMID 15571255.

External links

Deoxycytidine kinase at the US National Library of Medicine Medical Subject Headings (MeSH)

PDB gallery

1p5z: Structure of human dCK complexed with cytarabine and ADP-MG

1p60: Structure of human dCK complexed with 2'-Deoxycytidine and ADP, Space group C 2 2 21

1p61: Structure of human dCK complexed with 2'-Deoxycytidine and ADP, P 43 21 2 space group

1p62: Structure of human dCK complexed with gemcitabine and ADP-MG

2a2z: Crystal Structure of human deoxycytidine kinase in complex with deoxycytidine and uridine diphosphate

2a30: Crystal structure of human deoxycytidine kinase in complex with deoxycytidine

2a7q: Crystal structure of human dCK complexed with clofarabine and ADP

2no9: The structure of deoxycytidine kinase complexed with troxacitabine and ADP.

2noa: The structure of deoxycytidine kinase complexed with lamivudine and ADP.

Transferases: phosphorus-containing groups (EC 2.7)

2.7.1-2.7.4:
phosphotransferase/kinase
(PO₄)

2.7.1: OH acceptor	Hexo- Gluco- Fructo- Hepatic Galacto- Phosphofructo- 1 Liver Muscle Platelet 2 Riboflavin Shikimate Thymidine ADP-thymidine NAD⁺ Glycerol Pantothenate Mevalonate Pyruvate Deoxycytidine PFP Diacylglycerol Phosphoinositide 3 Class I PI 3 Class II PI 3 Sphingosine Glucose-1,6-bisphosphate synthase

2.7.2: COOH acceptor	Phosphoglycerate Aspartate kinase

2.7.3: N acceptor	Creatine

2.7.4: PO₄ acceptor	Phosphomevalonate Adenylate Nucleoside-diphosphate Uridylate Guanylate Thiamine-diphosphate

2.7.6: diphosphotransferase
(P₂O₇)

2.7.7: nucleotidyltransferase
(PO₄-nucleoside)

Polymerase

DNA polymerase	DNA-directed DNA polymerase I II III IV V RNA-directed DNA polymerase Reverse transcriptase Telomerase DNA nucleotidylexotransferase/Terminal deoxynucleotidyl transferase

RNA nucleotidyltransferase	RNA polymerase/DNA-directed RNA polymerase RNA polymerase I RNA polymerase II RNA polymerase III RNA polymerase IV Primase RNA-dependent RNA polymerase PNPase

Phosphorolytic
3' to 5' exoribonuclease

Nucleotidyltransferase

Guanylyltransferase

mRNA capping enzyme

Other

2.7.8: miscellaneous

Phosphatidyltransferases	CDP-diacylglycerol—glycerol-3-phosphate 3-phosphatidyltransferase CDP-diacylglycerol—serine O-phosphatidyltransferase CDP-diacylglycerol—inositol 3-phosphatidyltransferase CDP-diacylglycerol—choline O-phosphatidyltransferase

Glycosyl-1-phosphotransferase	N-acetylglucosamine-1-phosphate transferase

2.7.10-2.7.13: protein kinase
(PO₄; protein acceptor)

2.7.10: protein-tyrosine	see tyrosine kinases

2.7.11: protein-serine/threonine	see serine/threonine-specific protein kinases

2.7.12: protein-dual-specificity	see serine/threonine-specific protein kinases

2.7.13: protein-histidine	Protein-histidine pros-kinase Protein-histidine tele-kinase Histidine kinase

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Deoxycytidine kinase

Function

See also

References

Further reading

External links