Dermatophyte test medium

DTM plate on the right showing color change after two days' growth; Microsporum canis

Dermatophyte test medium (DTM) is a specialized agar used in medical mycology. It is based on Sabouraud's dextrose agar with added cycloheximide to inhibit saprotrophic growth, antibiotic to inhibit bacterial growth, and phenol red a pH indicator. The pH indicator is useful in distinguishing a dermatophyte fungus, which utilizes nitrogenous material for preferred metabolism, producing alkaline by-products, imparting a red color change to the medium. Typical saprotrophic fungi utilize carbohydrates in the medium producing acidic by-products and no red color change.

References

Growth media / agar plates

Selective media

Gram positive

Actinobacteria	Mycobacterium tuberculosis Löwenstein–Jensen medium Middlebrook 7H9 Broth Middlebrook 7H10 Agar Middlebrook 7H11 Agar Mycoplasma pneumoniae Eaton's agar

Firmicutes	Corynebacterium diphtheriae Hoyle's agar Enterococcus Bile esculin agar Lactobacillus MRS agar Lactococcus M17 agar Staphylococcus Mannitol salt agar

Gram negative

Alphaproteobacteria	Brucella Brucella agar

Betaproteobacteria	Neisseria Thayer-Martin agar

Gammaproteobacteria	Bordetella Bordet-Gengou agar Enterobacteriaceae VRBD agar Haemophilus influenzae/Legionella pneumophila Buffered charcoal yeast extract agar Pseudomonas aeruginosa Cetrimide agar Salmonella XLT agar DCA agar Salmonella/Shigella XLD agar

Differential media

Fungal media

Nonselective media

Other/ungrouped media

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