Cholate—CoA ligase

Cholate—CoA ligase
Identifiers
EC number	6.2.1.7
CAS number	9027-90-1
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Cholate—CoA ligase (EC 6.2.1.7, BAL, bile acid CoA ligase, bile acid coenzyme A ligase, choloyl-CoA synthetase, choloyl coenzyme A synthetase, cholic thiokinase, cholate thiokinase, cholic acid:CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl coenzyme A synthetase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA synthetase, THCA-CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate—CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate:CoA ligase (AMP-forming), cholyl-CoA synthetase, trihydroxycoprostanoyl-CoA synthetase) is an enzyme with systematic name cholate:CoA ligase (AMP-forming).^{[1][2][3][4][5][6][7]} This enzyme catalyses the following chemical reaction

(1) ATP + cholate + CoA $\rightleftharpoons$ AMP + diphosphate + choloyl-CoA

(2) ATP + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA $\rightleftharpoons$ AMP + diphosphate + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA

This enzyme requires Mg²⁺ for activity.

References

1. Elliott, W.H. (1956). "The enzymic activation of cholic acid by guinea-pig-liver microsomes". Biochem. J. 62 (3): 427–433. PMID 13303991.
2. Elliott, W.H. (1957). "The breakdown of adenosine triphosphate accompanying cholic acid activation by guinea-pig liver microsomes". Biochem. J. 65 (2): 315–321. PMID 13403911.
3. Prydz, K., Kase, B.F., Björkhem, I. and Pedersen, J.I. (1988). "Subcellular localization of 3α,7α-dihydroxy- and 3α,7α,12α-trihydroxy-5β-cholestanoyl-coenzyme A ligase(s) in rat liver". J. Lipid Res. 29 (8): 997–1004. PMID 3183523.
4. Schepers, L., Casteels, M., Verheyden, K., Parmentier, G., Asselberghs, S., Eyssen, H.J. and Mannaerts, G.P. (1989). "Subcellular distribution and characteristics of trihydroxycoprostanoyl-CoA synthetase in rat liver". Biochem. J. 257 (1): 221–229. PMID 2521999.
5. Mallonee, D.H., Adams, J.L. and Hylemon, P.B. (1992). "The bile acid-inducible baiB gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A ligase". J. Bacteriol. 174 (7): 2065–2071. PMID 1551828.
6. Wheeler, J.B., Shaw, D.R. and Barnes, S. (1997). "Purification and characterization of a rat liver bile acid coenzyme A ligase from rat liver microsomes". Arch. Biochem. Biophys. 348 (1): 15–24. doi:10.1006/abbi.1997.0391. PMID 9390170.
7. Falany, C.N., Xie, X., Wheeler, J.B., Wang, J., Smith, M., He, D. and Barnes, S. (2002). "Molecular cloning and expression of rat liver bile acid CoA ligase". J. Lipid Res. 43: 2062–2071. doi:10.1194/jlr.M200260-JLR200. PMID 12454267.

External links

• Cholate---CoA ligase at the US National Library of Medicine Medical Subject Headings (MeSH)