EPS8

Epidermal growth factor receptor pathway substrate 8
PDB rendering based on 1aoj.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2E8M​
Identifiers
Symbols	EPS8 ; DFNB102
External IDs	OMIM: 600206 MGI: 104684 HomoloGene: 3272 GeneCards: EPS8 Gene
Gene ontology Molecular function • actin binding • SH3/SH2 adaptor activity • protein binding • Rac GTPase binding Cellular component • brush border • cell cortex • postsynaptic density • NMDA selective glutamate receptor complex • cell junction • growth cone • vesicle • stereocilium • ruffle membrane • extracellular exosome Biological process • signal transduction • epidermal growth factor receptor signaling pathway • cell proliferation • adult locomotory behavior • regulation of cell shape • positive regulation of signal transduction • exit from mitosis • Rac protein signal transduction • regulation of actin filament length • actin cytoskeleton reorganization • dendritic cell migration • behavioral response to ethanol • barbed-end actin filament capping • actin filament bundle assembly • actin crosslink formation • actin polymerization-dependent cell motility Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	2059	13860
Ensembl	ENSG00000151491	ENSMUSG00000015766
UniProt	Q12929	Q08509
RefSeq (mRNA)	NM_004447	NM_001271587
RefSeq (protein)	NP_004438	NP_001258516
Location (UCSC)	Chr 12: 15.62 – 15.88 Mb	Chr 6: 137.48 – 137.65 Mb
PubMed search

Epidermal growth factor receptor kinase substrate 8 is an enzyme that in humans is encoded by the EPS8 gene.^[1][2]

Function

This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.^[2]

Clinical significance

Mutations in EPS8 cause congenital deafness .Behlouli A, Bonnet C, Abdi S, Bouaita A, Lelli A, Hardelin JP, Schietroma C, Rous Y, Louha M, Cheknane A, Lebdi H, Boudjelida K, Makrelouf M, Zenati A, Petit C (2014). "EPS8, encoding an actin-binding protein of cochlear hair cell stereocilia, is a new causal gene for autosomal recessive profound deafness". Orphanet Journal of Rare Diseases 9 (1): 55. doi:10.1186/1750-1172-9-55. PMC 4022326. PMID 24741995.

Interactions

EPS8 has been shown to interact with:

• ABI1,^[3][4][5]
• BAIAP2,^[6][7]
• DVL1,^[8]
• SHB,^[9]
• SHC1^[10]
• SOS1,^[3][11] and
• Src.^[12]

References

1. Wong WT, Carlomagno F, Druck T, Barletta C, Croce CM, Huebner K, Kraus MH, Di Fiore PP (October 1994). "Evolutionary conservation of the EPS8 gene and its mapping to human chromosome 12q23-q24". Oncogene 9 (10): 3057–61. PMID 8084614.
2. "Entrez Gene: EPS8 epidermal growth factor receptor pathway substrate 8".
3. Offenhäuser N, Borgonovo A, Disanza A, Romano P, Ponzanelli I, Iannolo G, Di Fiore PP, Scita G (January 2004). "The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway". Mol. Biol. Cell 15 (1): 91–8. doi:10.1091/mbc.E03-06-0427. PMC 307530. PMID 14565974.
4. Biesova Z, Piccoli C, Wong WT (January 1997). "Isolation and characterization of e3B1, an eps8 binding protein that regulates cell growth". Oncogene 14 (2): 233–41. doi:10.1038/sj.onc.1200822. PMID 9010225.
5. Lanzetti L, Rybin V, Malabarba MG, Christoforidis S, Scita G, Zerial M, Di Fiore PP (November 2000). "The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5". Nature 408 (6810): 374–7. doi:10.1038/35042605. PMID 11099046.
6. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
7. Funato Y, Terabayashi T, Suenaga N, Seiki M, Takenawa T, Miki H (August 2004). "IRSp53/Eps8 complex is important for positive regulation of Rac and cancer cell motility/invasiveness". Cancer Res. 64 (15): 5237–44. doi:10.1158/0008-5472.CAN-04-0327. PMID 15289329.
8. Inobe M, Katsube Ki, Miyagoe Y, Nabeshima Yi, Takeda S (December 1999). "Identification of EPS8 as a Dvl1-associated molecule". Biochem. Biophys. Res. Commun. 266 (1): 216–21. doi:10.1006/bbrc.1999.1782. PMID 10581192.
9. Karlsson T, Songyang Z, Landgren E, Lavergne C, Di Fiore PP, Anafi M, Pawson T, Cantley LC, Claesson-Welsh L, Welsh M (April 1995). "Molecular interactions of the Src homology 2 domain protein Shb with phosphotyrosine residues, tyrosine kinase receptors and Src homology 3 domain proteins". Oncogene 10 (8): 1475–83. PMID 7537362.
10. Matoskova B, Wong WT, Salcini AE, Pelicci PG, Di Fiore PP (July 1995). "Constitutive phosphorylation of eps8 in tumor cell lines: relevance to malignant transformation". Mol. Cell. Biol. 15 (7): 3805–12. PMC 230619. PMID 7791787.
11. Scita G, Nordstrom J, Carbone R, Tenca P, Giardina G, Gutkind S, Bjarnegård M, Betsholtz C, Di Fiore PP (September 1999). "EPS8 and E3B1 transduce signals from Ras to Rac". Nature 401 (6750): 290–3. doi:10.1038/45822. PMID 10499589.
12. Maa MC, Lai JR, Lin RW, Leu TH (July 1999). "Enhancement of tyrosyl phosphorylation and protein expression of eps8 by v-Src". Biochim. Biophys. Acta 1450 (3): 341–51. doi:10.1016/s0167-4889(99)00069-5. PMID 10395945.

Further reading

• Di Fiore PP, Scita G (2002). "Eps8 in the midst of GTPases". Int. J. Biochem. Cell Biol. 34 (10): 1178–83. doi:10.1016/S1357-2725(02)00064-X. PMID 12127568.
• Cardwell C (1977). "Student or worker? International Meeting of Student Nurses (European Group)". Nursing mirror and midwives journal 143 (19): 38. PMID 1049958.
• Castagnino P, Biesova Z, Wong WT, Fazioli F, Gill GN, Di Fiore PP (1995). "Direct binding of eps8 to the juxtamembrane domain of EGFR is phosphotyrosine- and SH2-independent". Oncogene 10 (4): 723–9. PMID 7532293.
• Karlsson T, Songyang Z, Landgren E, Lavergne C, Di Fiore PP, Anafi M, Pawson T, Cantley LC, Claesson-Welsh L, Welsh M (1995). "Molecular interactions of the Src homology 2 domain protein Shb with phosphotyrosine residues, tyrosine kinase receptors and Src homology 3 domain proteins". Oncogene 10 (8): 1475–83. PMID 7537362.
• Avantaggiato V, Torino A, Wong WT, Di Fiore PP, Simeone A (1995). "Expression of the receptor tyrosine kinase substrate genes eps8 and eps15 during mouse development". Oncogene 11 (6): 1191–8. PMID 7566980.
• Matoskova B, Wong WT, Salcini AE, Pelicci PG, Di Fiore PP (1995). "Constitutive phosphorylation of eps8 in tumor cell lines: relevance to malignant transformation". Mol. Cell. Biol. 15 (7): 3805–12. PMC 230619. PMID 7791787.
• Fazioli F, Minichiello L, Matoska V, Castagnino P, Miki T, Wong WT, Di Fiore PP (1993). "Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals". EMBO J. 12 (10): 3799–808. PMC 413663. PMID 8404850.
• Matòsková B, Wong WT, Seki N, Nagase T, Nomura N, Robbins KC, Di Fiore PP (1996). "RN-tre identifies a family of tre-related proteins displaying a novel potential protein binding domain". Oncogene 12 (12): 2563–71. PMID 8700515.
• Matòsková B, Wong WT, Nomura N, Robbins KC, Di Fiore PP (1996). "RN-tre specifically binds to the SH3 domain of eps8 with high affinity and confers growth advantage to NIH3T3 upon carboxy-terminal truncation". Oncogene 12 (12): 2679–88. PMID 8700527.
• Biesova Z, Piccoli C, Wong WT (1997). "Isolation and characterization of e3B1, an eps8 binding protein that regulates cell growth". Oncogene 14 (2): 233–41. doi:10.1038/sj.onc.1200822. PMID 9010225.
• Kishan KV, Scita G, Wong WT, Di Fiore PP, Newcomer ME (1997). "The SH3 domain of Eps8 exists as a novel intertwined dimer". Nat. Struct. Biol. 4 (9): 739–43. doi:10.1038/nsb0997-739. PMID 9303002.
• Maa MC, Lai JR, Lin RW, Leu TH (1999). "Enhancement of tyrosyl phosphorylation and protein expression of eps8 by v-Src". Biochim. Biophys. Acta 1450 (3): 341–51. doi:10.1016/S0167-4889(99)00069-5. PMID 10395945.
• Scita G, Nordstrom J, Carbone R, Tenca P, Giardina G, Gutkind S, Bjarnegård M, Betsholtz C, Di Fiore PP (1999). "EPS8 and E3B1 transduce signals from Ras to Rac". Nature 401 (6750): 290–3. doi:10.1038/45822. PMID 10499589.
• Lanzetti L, Rybin V, Malabarba MG, Christoforidis S, Scita G, Zerial M, Di Fiore PP (2000). "The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5". Nature 408 (6810): 374–7. doi:10.1038/35042605. PMID 11099046.
• Ion A, Crosby AH, Kremer H, Kenmochi N, Van Reen M, Fenske C, Van Der Burgt I, Brunner HG, Montgomery K, Kucherlapati RS, Patton MA, Page C, Mariman E, Jeffery S (2000). "Detailed mapping, mutation analysis, and intragenic polymorphism identification in candidate Noonan syndrome genes MYL2, DCN, EPS8, and RPL6". J. Med. Genet. 37 (11): 884–6. doi:10.1136/jmg.37.11.884. PMC 1734479. PMID 11185075.
• Scita G, Tenca P, Areces LB, Tocchetti A, Frittoli E, Giardina G, Ponzanelli I, Sini P, Innocenti M, Di Fiore PP (2001). "An effector region in Eps8 is responsible for the activation of the Rac-specific GEF activity of Sos-1 and for the proper localization of the Rac-based actin–polymerizing machine". J. Cell Biol. 154 (5): 1031–44. doi:10.1083/jcb.200103146. PMC 2196181. PMID 11524436.