Ent-kaurenoic acid oxidase

Ent-kaurenoic acid oxidase
Identifiers
EC number	1.14.13.79
CAS number	337507-95-6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Ent-kaurenoic acid oxidase (EC 1.14.13.79) is an enzyme with systematic name ent-kaur-16-en-19-oate,NADPH:oxygen oxidoreductase (hydroxylating).^[1] This enzyme catalyses the following chemical reaction

ent-kaur-16-en-19-oate + 3 NADPH + 3 H⁺ + 3 O₂ $\rightleftharpoons$ gibberellin A12 + 3 NADP⁺ + 4 H₂O (overall reaction)

(1a) ent-kaur-16-en-19-oate + NADPH + H⁺ + O₂ $\rightleftharpoons$ ent-7alpha-hydroxykaur-16-en-19-oate + NADP⁺ + H₂O

(1b) ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + H⁺ + O₂ $\rightleftharpoons$ gibberellin A12 aldehyde + NADP⁺ + 2 H₂O

(1c) gibberellin A12 aldehyde + NADPH + H⁺ + O₂ $\rightleftharpoons$ gibberellin A12 + NADP⁺ + H₂O

Ent-kaurenoic acid oxidase requires cytochrome P450.

References

1. Helliwell, C.A., Chandler, P.M., Poole, A., Dennis, E.S. and Peacock, W.J. (2001). "The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway". Proc. Natl. Acad. Sci. USA 98 (4): 2065–2070. doi:10.1073/pnas.041588998. PMC 29382. PMID 11172076.

External links

• Ent-kaurenoic acid oxidase at the US National Library of Medicine Medical Subject Headings (MeSH)