Extracellular polymeric substance

Extracellular polymeric substance matrix formation in a biofilm

Extracellular polymeric substances (EPS), are high-molecular weight compounds secreted by microorganisms into their environment.[1] EPS establish the functional and structural integrity of biofilms, and are considered the fundamental component that determines the physiochemical properties of a biofilm.[2]

EPS are mostly composed of polysaccharides (exopolysaccharides) and proteins, but include other macro-molecules such as DNA, lipids and humic substances. EPS are the construction material of bacterial settlements and either remain attached to the cell's outer surface, or are secreted into its growth medium. These compounds are important in biofilm formation and cells attachment to surfaces. EPS constitutes 50% to 90% of a biofilm's total organic matter.[2][3][4]

Exopolysaccharides are high-molecular-weight polymers that are composed of sugar residues and are secreted by a microorganism into the surrounding environment. Microorganisms synthesize a wide spectrum of multifunctional polysaccharides including intracellular polysaccharides, structural polysaccharides and extracellular polysaccharides or exopolysaccharides (EPS). Exopolysaccharides generally consist of monosaccharides and some non-carbohydrate substituents (such as acetate, pyruvate, succinate, and phosphate). Owing to the wide diversity in composition, exopolysaccharides have found multifarious applications in various food and pharmaceutical industries. Many microbial EPS provide properties that are almost identical to the gums currently in use. With innovative approaches, efforts are underway to supersede the traditionally used plant and algal gums by their microbial counterparts. Moreover, considerable progress has been made in discovering and developing new microbial EPS that possess novel industrial significance.[5]

Function

The exopolysaccharides of some strains of lactic acid bacteria, e.g., Lactococcus lactis subsp. cremoris, contribute a gelatinous texture to fermented milk products (e.g., Viili), and these polysaccharides are also digestible.[6][7]

Capsular exopolysaccharides can protect pathogenic bacteria and contribute to their pathogenicity. Attachment of nitrogen-fixing bacteria to plant roots and soil particles, which is important for colonisation of rhizosphere and roots and for infection of the plant, can be mediated by exopolysaccharides as well. An example for industrial use of exopolysaccharides is the application of dextran in panettone and other breads in the bakery industry.[8] Exopolysaccharides also have an important role in endodontic infections.

List

Succinoglycan from Sinorhizobium meliloti

See also

References

  1. ↑ Staudt C, Horn H, Hempel DC, Neu TR (2004). "Volumetric measurements of bacterial cells and extracellular polymeric substance glycoconjugates in biofilms". Biotechnol. Bioeng. 88 (5): 585–92. doi:10.1002/bit.20241. PMID 15470707.
  2. 1 2 Flemming, Hans-Curt; Wingender, Jost; Griebe, Thomas; Mayer, Christian (December 21, 2000), "Physico-Chemical Properties of Biofilms", in L. V. Evans, Biofilms: Recent Advances in their Study and Control, CRC Press, p. 20, ISBN 978-9058230935
  3. ↑ Donlan RM (2002). "Biofilms: microbial life on surfaces". Emerging Infect. Dis. 8 (9): 881–90. doi:10.3201/eid0809.020063. PMC 2732559. PMID 12194761.
  4. ↑ Donlan RM, Costerton JW (2002). "Biofilms: survival mechanisms of clinically relevant microorganisms". Clin. Microbiol. Rev. 15 (2): 167–93. doi:10.1128/CMR.15.2.167-193.2002. PMC 118068. PMID 11932229.
  5. ↑ Suresh and Mody (2009). "Microbial Exopolysaccharides: Variety and Potential Applications". Microbial Production of Biopolymers and Polymer Precursors. Caister Academic Press. ISBN 978-1-904455-36-3.
  6. ↑ Welman AD (2009). "Exploitation of Exopolysaccharides from lactic acid bacteria". Bacterial Polysaccharides: Current Innovations and Future Trends. Caister Academic Press. ISBN 978-1-904455-45-5.
  7. ↑ Ljungh A, Wadstrom T (editors) (2009). Lactobacillus Molecular Biology: From Genomics to Probiotics. Caister Academic Press. ISBN 978-1-904455-41-7.
  8. ↑ Ullrich M (editor) (2009). Bacterial Polysaccharides: Current Innovations and Future Trends. Caister Academic Press. ISBN 978-1-904455-45-5.

External links


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