Flavonoid 3',5'-hydroxylase

Flavanoid 3',5'-hydroxylase
Identifiers
EC number	1.14.13.88
CAS number	94047-23-1
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Flavanoid 3',5'-hydroxylase (EC 1.14.13.88) is an enzyme with systematic name flavanone,NADPH:oxygen oxidoreductase.^[1][2][3] This enzyme catalyses the following chemical reaction

flavanone + 2 NADPH + 2 H⁺ + 2 O₂ $\rightleftharpoons$ 3',5'-dihydroxyflavanone + 2 NADP⁺ + 2 H₂O (overall reaction)

(1a) flavanone + NADPH + H⁺ + O₂ $\rightleftharpoons$ 3'-hydroxyflavanone + NADP⁺ + H₂O

(1b) 3'-hydroxyflavanone + NADPH + H⁺ + O₂ $\rightleftharpoons$ 3',5'-dihydroxyflavanone + NADP⁺ + H₂O

Flavonoid 3',5'-hydroxylase is a heme-thiolate protein (P-450).

References

1. Menting, J., Scopes, R.K. and Stevenson, T.W. (1994). "Characterization of flavonoid 3′,5′-hydroxylase in microsomal membrane fraction of Petunia hybrida flowers". Plant Physiol. 106 (2): 633–642. doi:10.1104/pp.106.2.633. PMC 159570. PMID 12232356.
2. Shimada, Y., Nakano-Shimada, R., Ohbayashi, M., Okinaka, Y., Kiyokawa, S. and Kikuchi, Y. (1999). "Expression of chimeric P450 genes encoding flavonoid-3′, 5′-hydroxylase in transgenic tobacco and petunia plants¹". FEBS Lett. 461 (3): 241–245. doi:10.1016/s0014-5793(99)01425-8. PMID 10567704.
3. de Vetten, N., ter Horst, J., van Schaik, H.P., de Boer, A., Mol, J. and Koes, R. (1999). "A cytochrome b₅ is required for full activity of flavonoid 3′, 5′-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors". Proc. Natl. Acad. Sci. USA 96 (2): 778–783. doi:10.1073/pnas.96.2.778. PMC 15213. PMID 9892710.

External links

• Flavanoid 3',5'-hydroxylase at the US National Library of Medicine Medical Subject Headings (MeSH)