GCA (gene)

Grancalcin, EF-hand calcium binding protein
PDB rendering based on 1f4o.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1F4O​, 1F4Q​, 1K94​, 1K95​
Identifiers
Symbols	GCA ; GCL
External IDs	OMIM: 607030 MGI: 1918521 HomoloGene: 22702 GeneCards: GCA Gene
Gene ontology Molecular function • calcium-dependent cysteine-type endopeptidase activity • calcium ion binding • protein binding • protein homodimerization activity • protein heterodimerization activity Cellular component • cytoplasm • plasma membrane • extracellular exosome Biological process • proteolysis • membrane fusion Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	25801	227960
Ensembl	ENSG00000115271	ENSMUSG00000026893
UniProt	P28676	Q8VC88
RefSeq (mRNA)	NM_012198	NM_145523
RefSeq (protein)	NP_036330	NP_663498
Location (UCSC)	Chr 2: 162.32 – 162.37 Mb	Chr 2: 62.66 – 62.69 Mb
PubMed search

Grancalcin is a protein that in humans is encoded by the GCA gene.^[1][2][3][4]

This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.^[4]

Interactions

GCA (gene) has been shown to interact with SRI.^[3]

References

1. Boyhan A, Casimir CM, French JK, Teahan CG, Segal AW (Mar 1992). "Molecular cloning and characterization of grancalcin, a novel EF-hand calcium-binding protein abundant in neutrophils and monocytes". J Biol Chem 267 (5): 2928–33. PMID 1737748.
2. Teahan CG, Totty NF, Segal AW (Oct 1992). "Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils". Biochem J. 286. ( Pt 2): 549–54. PMC 1132932. PMID 1530588.
3. Hansen C, Tarabykina S, la Cour JM, Lollike K, Berchtold MW (Jun 2003). "The PEF family proteins sorcin and grancalcin interact in vivo and in vitro". FEBS Lett 545 (2-3): 151–4. doi:10.1016/S0014-5793(03)00518-0. PMID 12804766.
4. "Entrez Gene: GCA grancalcin, EF-hand calcium binding protein".

Further reading

• Lim J, Hao T, Shaw C; et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.". Cell 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569.
• Rual JF, Venkatesan K, Hao T; et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Hillier LW, Graves TA, Fulton RS; et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4.". Nature 434 (7034): 724–31. doi:10.1038/nature03466. PMID 15815621.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Jia J, Borregaard N, Lollike K, Cygler M (2002). "Structure of Ca(2+)-loaded human grancalcin.". Acta Crystallogr. D Biol. Crystallogr. 57 (Pt 12): 1843–9. doi:10.1107/S0907444901016511. PMID 11717497.
• Lollike K, Johnsen AH, Durussel I; et al. (2001). "Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner.". J. Biol. Chem. 276 (21): 17762–9. doi:10.1074/jbc.M100965200. PMID 11279160.
• Jia J, Han Q, Borregaard N; et al. (2000). "Crystal structure of human grancalcin, a member of the penta-EF-hand protein family.". J. Mol. Biol. 300 (5): 1271–81. doi:10.1006/jmbi.2000.3925. PMID 10903868.
• Maki M, Narayana SV, Hitomi K (1998). "A growing family of the Ca2+-binding proteins with five EF-hand motifs.". Biochem. J. 328. ( Pt 2): 718–20. PMC 1218977. PMID 9441591.