Iridoviridae
| Iridoviridae | |
|---|---|
| Virus classification | |
| Group: | Group I (dsDNA) |
| Family: | Iridoviridae |
| Genera | |
Iridoviridae is a family of viruses with double-stranded DNA genomes. Amphibia, fish, invertebrates, lepidoptera, and orthoptera insects serve as natural hosts. There are currently 11 species in this family, divided among 5 genera.[1][2] The name is derived from Iris the Greek goddess of the rainbow. This name was chosen because of the "rainbow-like" iridescence observed in heavily infected insects and pelleted samples of invertebrate iridoviruses.
Nomenclature
The term "Iridovirus" has an ambiguous meaning. It frequently appears in the literature as a description of any member of the Iridoviridae family. Yet it also is the name of a particular genus within Iridoviridae.
Taxonomy
Group: dsDNA
- Family: Iridoviridae
- Genus: Iridovirus
- Invertebrate iridescent virus 1
- Invertebrate iridescent virus 6
- Genus: Megalocytivirus
- Infectious spleen and kidney necrosis virus
- Genus: Ranavirus
- Ambystoma tigrinum virus
- Bohle iridovirus
- Epizootic haematopoietic necrosis virus
- European catfish virus
- Frog virus 3
- Santee-Cooper ranavirus
Structure
The virions are icosahedral with triangulation number (T) = 189–217, 120–350 nm in diameter and made up of three domains: an outer proteinaceous capsid, an intermediate lipid membrane, and a central core containing DNA-protein complexes. Some of the viruses also have an outer envelope. The presence or absence of an envelope depends on whether they budded from the cell membrane (enveloped viruses) or were arranged in paracrystalline arrays within the host cell cytoplasm and then were released by cell lysis (unenveloped viruses).
The linear genome varies between 150 and 303 kilobases in length. It contains terminal and redundant sequences and is circularly permuted.
Members of this family differ in their degree of genome methylation. The genera Chloriridovirus and Iridovirus lack a highly methylated genome. Members of the Lymphocystivirus, Megalocytivirus, and Ranavirus genera have genomes with about 25% of their cytosine residues methylated by a virally encoded DNA methyltransferase.
| Genus | Structure | Symmetry | Capsid | Genomic Arrangement | Genomic Segmentation |
|---|---|---|---|---|---|
| Lymphocystivirus | Polyhedral | T=189-217 | Linear | Monopartite | |
| Megalocytivirus | Polyhedral | T=189-217 | Linear | Monopartite | |
| Ranavirus | Polyhedral | T=133 or 147 | Linear | Monopartite | |
| Iridovirus | Polyhedral | T=147 | Linear | Monopartite | |
| Chloriridovirus | Polyhedral | T=189-217 | Linear | Monopartite |
Gene expression
Similar to the herpes viruses, transcription occurs in three stages: immediate-early, delayed-early, and late. Positive induction and negative feedback mechanisms exist in each stage, mediated by products of the other stages.
Replication
Virus particles enter the cell and uncoating occurs. The viral DNA is transported to the host cell nucleus, where it is transcribed by host RNA polymerase II modified by the virus. Meanwhile, host macromolecular synthesis is stopped.
Parental DNA produces a genome which is then the template for replication in the cytoplasm. Large concatemers of viral DNA are formed by recombination in the cytoplasm. Packaging of the new genomes into virions occurs in the cytoplasm and the virus is released either by budding from the cell membrane or cell lysis.
| Genus | Host Details | Tissue Tropism | Entry Details | Release Details | Replication Site | Assembly Site | Transmission |
|---|---|---|---|---|---|---|---|
| Lymphocystivirus | Fish | None | Cell receptor endocytosis | Lysis; budding | Nucleus | Cytoplasm | Unknown |
| Megalocytivirus | Fish | None | Cell receptor endocytosis | Lysis; budding | Nucleus | Cytoplasm | Unknown |
| Ranavirus | Frogs; snakes | None | Cell receptor endocytosis | Lysis; budding | Nucleus | Cytoplasm | Contact |
| Iridovirus | Insects | None | Cell receptor endocytosis | Lysis; budding | Nucleus | Cytoplasm | Contact |
| Chloriridovirus | Diptera with aquatic larval stage, mainly mosquitoes | None | Cell receptor endocytosis | Budding | Nucleus | Cytoplasm | Unknown |
Pathogenesis
Little is known about the pathogenesis of iridoviruses. The pathogenesis is, however, temperature dependent and iridoviruses are thus confined to poikilothermic hosts.
Host range
Members of the Iridoviridae family infect mainly invertebrates, but also some vertebrate species such as fish, amphibians and reptiles.
References
- ↑ "Viral Zone". ExPASy. Retrieved 15 June 2015.
- 1 2 ICTV. "Virus Taxonomy: 2014 Release". Retrieved 15 June 2015.
MicrobiologyBytes: Iridoviruses, archived from the original on February 24, 2007, retrieved 2007-03-06
Viral Bioinformatics Resource Center & Viral Bioinformatics – Canada, University of Victoria, archived from the original on August 17, 2007, retrieved 2007-03-06
The Universal Virus Database of the International Committee on Taxonomy of Viruses, retrieved 2007-03-06