Isoflavone 2'-hydroxylase

Isoflavone 2'-hydroxylase
Identifiers
EC number	1.14.13.89
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

In enzymology, an isoflavone 2'-hydroxylase (EC 1.14.13.89) is an enzyme that catalyzes the chemical reaction

an isoflavone + NADPH + H⁺ + O₂ $\rightleftharpoons$ a 2'-hydroxyisoflavone + NADP⁺ + H₂O

The 4 substrates of this enzyme are isoflavone, NADPH, H⁺, and O₂, whereas its 3 products are 2'-hydroxyisoflavone, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is isoflavone,NADPH:oxygen oxidoreductase (2'-hydroxylating). Other names in common use include isoflavone 2'-monooxygenase, CYP81E1, and CYP Ge-3. This enzyme participates in isoflavonoid biosynthesis.

References

• Akashi T, Aoki T, Ayabe S (1998). "CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase". Biochem. Biophys. Res. Commun. 251 (1): 67–70. doi:10.1006/bbrc.1998.9414. PMID 9790908.