Isoleucine N-monooxygenase

Isoleucine N-monooxygenase
Identifiers
EC number	1.14.13.117
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Isoleucine N-monooxygenase (EC 1.14.13.117, CYP79D3, CYP79D4) is an enzyme with systematic name L-isoleucine,NADPH:oxygen oxidoreductase (N-hydroxylating).^[1][2] This enzyme catalyses the following chemical reaction

L-isoleucine + 2 O₂ + 2 NADPH + 2 H⁺ $\rightleftharpoons$ (E)-2-methylbutanal oxime + 2 NADP⁺ + CO₂ + 3 H₂O (overall reaction)

(1a) L-isoleucine + O₂ + NADPH + H⁺ $\rightleftharpoons$ N-hydroxy-L-isoleucine + NADP⁺ + H₂O

(1b) N-hydroxy-L-isoleucine + O₂ + NADPH + H+ $\rightleftharpoons$ N,N-dihydroxy-L-isoleucine + NADP⁺ + H₂O

(1c) N,N-dihydroxy-L-isoleucine $\rightleftharpoons$ (E)-2-methylbutanal oxime + CO₂ + H₂O (spontaneous reaction)

Isoleucine N-monooxygenase is a heme-thiolate protein (P-450).

References

1. Andersen, M.D., Busk, P.K., Svendsen, I. and Moller, B.L. (2000). "Cytochromes P-450 from cassava (Manihot esculenta Crantz) catalyzing the first steps in the biosynthesis of the cyanogenic glucosides linamarin and lotaustralin. Cloning, functional expression in Pichia pastoris, and substrate specificity of the isolated recombinant enzymes". J. Biol. Chem. 275 (3): 1966–1975. doi:10.1074/jbc.275.3.1966. PMID 10636899.
2. Forslund, K., Morant, M., Jorgensen, B., Olsen, C.E., Asamizu, E., Sato, S., Tabata, S. and Bak, S. (2004). "Biosynthesis of the nitrile glucosides rhodiocyanoside A and D and the cyanogenic glucosides lotaustralin and linamarin in Lotus japonicus". Plant Physiol. 135 (1): 71–84. doi:10.1104/pp.103.038059. PMC 429334. PMID 15122013.

External links

• Isoleucine N-monooxygenase at the US National Library of Medicine Medical Subject Headings (MeSH)