MED22

Mediator complex subunit 22
Identifiers
Symbols MED22 ; MED24; SURF5; surf-5
External IDs OMIM: 185641 MGI: 98446 HomoloGene: 4913 GeneCards: MED22 Gene
RNA expression pattern
More reference expression data
Orthologs
Species Human Mouse
Entrez 6837 20933
Ensembl ENSG00000148297 ENSMUSG00000015776
UniProt Q15528 Q62276
RefSeq (mRNA) NM_006752 NM_001033908
RefSeq (protein) NP_598395 NP_001029080
Location (UCSC) Chr 9:
133.34 – 133.35 Mb		 Chr 2:
26.91 – 26.91 Mb
PubMed search

Mediator of RNA polymerase II transcription subunit 22 is an enzyme that in humans is encoded by the MED22 gene.[1][2][3]

Function

This gene is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene.[3]

Interactions

MED22 has been shown to interact with MED30.[4]

Model organisms

Model organisms have been used in the study of MED22 function. A conditional knockout mouse line called Med22tm1a(EUCOMM)Wtsi was generated at the Wellcome Trust Sanger Institute.[5] Male and female animals underwent a standardized phenotypic screen[6] to determine the effects of deletion.[7][8][9][10] Additional screens performed: - In-depth immunological phenotyping[11]

References

  1. ↑ Yon J, Jones T, Garson K, Sheer D, Fried M (Mar 1993). "The organization and conservation of the human Surfeit gene cluster and its localization telomeric to the c-abl and can proto-oncogenes at chromosome band 9q34.1". Human Molecular Genetics 2 (3): 237–40. doi:10.1093/hmg/2.3.237. PMID 8499913.
  2. ↑ Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Molecular Cell 14 (5): 685–91. doi:10.1016/j.molcel.2004.05.006. PMID 15175163.
  3. 1 2 "Entrez Gene: SURF5 surfeit 5".
  4. ↑ Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW (Apr 2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". The Journal of Biological Chemistry 278 (17): 15123–7. doi:10.1074/jbc.C300054200. PMID 12584197.
  5. ↑ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica 88. doi:10.1111/j.1755-3768.2010.4142.x.
  6. 1 2 "International Mouse Phenotyping Consortium".
  7. ↑ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
  8. ↑ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
  9. ↑ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
  10. ↑ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
  11. 1 2 "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

  • De Falco S, Russo G, Angiolillo A, Pietropaolo C (Apr 1993). "Human L7a ribosomal protein: sequence, structural organization, and expression of a functional gene". Gene 126 (2): 227–35. doi:10.1016/0378-1119(93)90371-9. PMID 8482538. 
  • Garson K, Duhig T, Armes N, Colombo P, Fried M (Nov 1995). "Surf5: a gene in the tightly clustered mouse surfeit locus is highly conserved and transcribed divergently from the rpL7A (Surf3) gene". Genomics 30 (2): 163–70. doi:10.1006/geno.1995.9889. PMID 8586415. 
  • Garson K, Duhig T, Fried M (1997). "Tissue-specific processing of the Surf-5 and Surf-4 mRNAs". Gene Expression 6 (4): 209–18. PMID 9196076. 
  • Duhig T, Ruhrberg C, Mor O, Fried M (Aug 1998). "The human Surfeit locus". Genomics 52 (1): 72–8. doi:10.1006/geno.1998.5372. PMID 9740673. 
  • Angiolillo A, Russo G, Porcellini A, Smaldone S, D'Alessandro F, Pietropaolo C (Feb 2002). "The human homologue of the mouse Surf5 gene encodes multiple alternatively spliced transcripts". Gene 284 (1-2): 169–78. doi:10.1016/S0378-1119(02)00379-7. PMID 11891058. 
  • Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW (Apr 2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". The Journal of Biological Chemistry 278 (17): 15123–7. doi:10.1074/jbc.C300054200. PMID 12584197. 
  • Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW (Dec 2003). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex". The Journal of Biological Chemistry 278 (50): 49671–4. doi:10.1074/jbc.C300444200. PMID 14576168. 
  • Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW (Feb 2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2". The Journal of Biological Chemistry 279 (7): 5846–51. doi:10.1074/jbc.M312523200. PMID 14638676. 
  • Hillman RT, Green RE, Brenner SE (2005). "An unappreciated role for RNA surveillance". Genome Biology 5 (2): R8. doi:10.1186/gb-2004-5-2-r8. PMC 395752. PMID 14759258. 


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