Molybdopterin synthase sulfurtransferase

Molybdopterin synthase sulfurtransferase (EC 2.8.1.11, adenylyltransferase and sulfurtransferase MOCS3, Cnx5 (gene), molybdopterin synthase sulfurylase) is an enzyme with systematic name persulfurated L-cysteine desulfurase:(molybdopterin-synthase sulfur-carrier protein)-Gly-Gly sulfurtransferase.^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

[molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine

\rightleftharpoons

AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + cysteine desulfurase

The enzyme transfers sulfur to form a thiocarboxylate moiety on the C-terminal glycine of the small subunit of molybdopterin synthase.

References

↑ Matthies, A., Nimtz, M. and Leimkuhler, S. (2005). "Molybdenum cofactor biosynthesis in humans: identification of a persulfide group in the rhodanese-like domain of MOCS3 by mass spectrometry". Biochemistry 44: 7912–7920. doi:10.1021/bi0503448. PMID 15910006.
↑ Leimkuhler, S. and Rajagopalan, K.V. (2001). "A sulfurtransferase is required in the transfer of cysteine sulfur in the in vitro synthesis of molybdopterin from precursor Z in Escherichia coli". J. Biol. Chem. 276 (25): 22024–22031. doi:10.1074/jbc.M102072200. PMID 11290749.
↑ Hanzelmann, P., Dahl, J.U., Kuper, J., Urban, A., Muller-Theissen, U., Leimkuhler, S. and Schindelin, H. (2009). "Crystal structure of YnjE from Escherichia coli, a sulfurtransferase with three rhodanese domains". Protein Sci. 18 (12): 2480–2491. doi:10.1002/pro.260. PMID 19798741.
↑ Dahl, J.U., Urban, A., Bolte, A., Sriyabhaya, P., Donahue, J.L., Nimtz, M., Larson, T.J. and Leimkuhler, S. (2011). "The identification of a novel protein involved in molybdenum cofactor biosynthesis in Escherichia coli". J. Biol. Chem. 286 (41): 35801–35812. doi:10.1074/jbc.M111.282368. PMID 21856748.

External links

Molybdopterin synthase sulfurtransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferases: sulfur-containing group (EC 2.8)

2.8.1: Sulfurtransferases	Thiosulfate sulfurtransferase Rhodanese 3-mercaptopyruvate sulfurtransferase thiosulfate—thiol sulfurtransferase tRNA sulfurtransferase thiosulfate—dithiol sulfurtransferase biotin synthase cysteine desulfurase

2.8.2: Sulfotransferases	Alcohol sulfotransferase Tyrosylprotein sulfotransferase Aryl sulfotransferase

2.8.3: CoA-transferases	Propionate CoA-transferase

2.8.4: Alkylthio	Coenzyme-B sulfoethylthiotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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