Monovalent cation:Proton antiporter-2

The Monovalent Cation:Proton Antiporter-2 (CPA2) Family (TC# 2.A.37) is a moderately large family of transporters belonging to the CPA superfamily. Members of the CPA2 family have been found in bacteria, archaea and eukaryotes. The proteins of the CPA2 family consist of between 333 and 900 amino acyl residues and exhibit 10-14 transmembrane α-helical spanners (TMSs).[1][2]

Homology

Several organisms possess multiple CPA2 paralogues. Thus, E. coli has three, Methanococcus jannaschii has four and Synechocystis sp. has five paralogues. The potassium efflux system, Kef, protects bacteria against the detrimental effects of electrophilic compounds via acidification of the cytoplasm. Kef is inhibited by glutathione (GSH) but activated by glutathione-S-conjugates (GS-X) formed in the presence of electrophiles. GSH and GS-X bind to overlapping sites on Kef, which are located in a cytosolic regulatory domain.[1]

Function

Among the functionally well-characterized members of the family are:

  1. KefB/KefC K+ efflux proteins of E. coli (i.e., TC# 2.A.37.1.3 and TC# 2.A.37.1.1, respectively), which may be capable of catalyzing both K+/H+ antiport and K+ uniport, depending on conditions [3][4][5]
  2. Na+/H+ antiporter of Enterococcus hirae (i.e., NapA, TC# 2.A.37.2.1) [6]
  3. K+/H+ antiporter of S. cerevisiae (i.e., Kha1, TC# 2.A.37.4.1). It has been proposed that under normal physiological conditions, these proteins may function by essentially the same mechanism.

KefC and KefB of E. coli are responsible for glutathione-gated K+ efflux.[7][8] Each of these proteins consists of a transmembrane hydrophobic N-terminal domain, and a lesser conserved C-terminal hydrophilic domain. Each protein interacts with a second protein encoded by genes that overlap the gene encoding the primary transporter. The KefC ancillary protein is YabF while the KefB ancillary protein is YheR. These ancillary proteins stimulate transport activity about 10-fold.[9] These proteins are important for cell survival during exposure to toxic metabolites, possibly because they can release K+, allowing H+ uptake. Activation of the KefB or KefC K+ efflux system only occurs in the presence of glutathione and a reactive electrophile such as methylglyoxal or N-ethylmaleimide. Formation of the methylglyoxal-glutathione conjugate, S-lactoylglutathione, is catalyzed by glyoxalase I, and S-lactoylglutathione activates KefB and KefC.[10] H+ uptake (acidification of the cytoplasm) accompanying or following K+ efflux may serve as a further protective mechanism against electrophile toxicity.[4][7][8][11] Inhibition of transport by glutathione was enhanced by NADH.[12]

Gram-negative bacteria are protected against toxic electrophilic compounds by glutathione-gated potassium efflux systems (Kef) that modulate cytoplasmic pH. Roosild et al. (2010) have elucidated the mechanism of gating through structural and functional analysis of the E. coli KefC. The revealed mechanism can explain how subtle chemical differences in glutathione derivatives can produce opposite effects on channel function.[13] Kef channels are regulated by potassium transport and NAD-binding (KTN) domains that sense both reduced glutathione, which inhibits Kef activity, and glutathione adducts that form during electrophile detoxification and activate Kef. Roosild et al. (2010) found that reduced glutathione stabilizes an inter-domain association between two KTN folds, whereas large adducts sterically disrupt this interaction. F441 is identified as the pivotal residue discriminating between reduced glutathione and its conjugates. They demonstrated a major structural change on the binding of an activating ligand to a KTN-domain protein.[13]

The MagA protein of Magnetospirillum sp. strain AMB-1 has been reported to be required for synthesis of bacterial magnetic particles. The magA gene is subject to transcriptional activation by an iron deficiency.[14] However, a more recent report has shown that magA mutants of both Magnetospirillum magneticum AMB-1 and M. gryphiswaldense MSR-1 formed wild-type-like magnetosomes without a growth defect.[15] Its transport function is not known. The GerN and GrmA proteins of Bacillus cereus and Bacillus megaterium, respectively, are spore germination proteins that can exchange Na+ for H+ and/or K+.[16] The AmhT homologue of Bacillus pseudofirmus transports both K+ and NH4+, influences ammonium homeostasis, and is required for normal sporulation and germination. The identification of these proteins as members of the CPA2 family reveals that monovalent cation transport is required for Bacillus spore formation and germination.[17]

Transport Reaction

The generalized transport reaction catalyzed by members of the CPA2 family is:

M+ (in) + nH+ (out) ⇌ M+ (out) + nH+ (in).
(The carrier-mediated mode)

Some members may also catalyze:

M+ (in) ⇌ M+ (out).
(The channel-mediated mode)

See also

Further reading

References

  1. 1 2 Healy, Jessica; Ekkerman, Silvia; Pliotas, Christos; Richard, Morgiane; Bartlett, Wendy; Grayer, Samuel C.; Morris, Garrett M.; Miller, Samantha; Booth, Ian R. (2014-04-01). "Understanding the structural requirements for activators of the Kef bacterial potassium efflux system". Biochemistry 53 (12): 1982–1992. doi:10.1021/bi5001118. ISSN 1520-4995. PMC 4004266. PMID 24601535.
  2. ↑ "2.A.37 The Monovalent Cation:Proton Antiporter-2 (CPA2) Family". Transporter Classification Database. Retrieved 2016-03-16.
  3. ↑ Bakker, E. P.; Borchard, A.; Michels, M.; Altendorf, K.; Siebers, A. (1987-09-01). "High-affinity potassium uptake system in Bacillus acidocaldarius showing immunological cross-reactivity with the Kdp system from Escherichia coli". Journal of Bacteriology 169 (9): 4342–4348. ISSN 0021-9193. PMC 213750. PMID 2957359.
  4. 1 2 Booth, I.R.; Jones, M.A.; McLaggan, D; Nikolaev, Y (1996). Konings, W.N., ed. Bacterial Ion Channels. Transport Processes in Eukaryotic and Prokaryotic Organisms 2 (New York: Elsevier Press). ISBN 978-0-444-82442-4.
  5. ↑ Munro, A. W.; Ritchie, G. Y.; Lamb, A. J.; Douglas, R. M.; Booth, I. R. (1991-03-01). "The cloning and DNA sequence of the gene for the glutathione-regulated potassium-efflux system KefC of Escherichia coli". Molecular Microbiology 5 (3): 607–616. ISSN 0950-382X. PMID 2046548.
  6. ↑ Waser, M.; Hess-Bienz, D.; Davies, K.; Solioz, M. (1992-03-15). "Cloning and disruption of a putative NaH-antiporter gene of Enterococcus hirae". The Journal of Biological Chemistry 267 (8): 5396–5400. ISSN 0021-9258. PMID 1312090.
  7. 1 2 Ferguson, G. P.; Munro, A. W.; Douglas, R. M.; McLaggan, D.; Booth, I. R. (1993-09-01). "Activation of potassium channels during metabolite detoxification in Escherichia coli". Molecular Microbiology 9 (6): 1297–1303. ISSN 0950-382X. PMID 7934942.
  8. 1 2 Ferguson, G. P.; Nikolaev, Y.; McLaggan, D.; Maclean, M.; Booth, I. R. (1997-02-01). "Survival during exposure to the electrophilic reagent N-ethylmaleimide in Escherichia coli: role of KefB and KefC potassium channels". Journal of Bacteriology 179 (4): 1007–1012. ISSN 0021-9193. PMC 178791. PMID 9023177.
  9. ↑ Miller, S.; Ness, L. S.; Wood, C. M.; Fox, B. C.; Booth, I. R. (2000-11-01). "Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli". Journal of Bacteriology 182 (22): 6536–6540. ISSN 0021-9193. PMC 94807. PMID 11053405.
  10. ↑ MacLean, M. J.; Ness, L. S.; Ferguson, G. P.; Booth, I. R. (1998-02-01). "The role of glyoxalase I in the detoxification of methylglyoxal and in the activation of the KefB K+ efflux system in Escherichia coli". Molecular Microbiology 27 (3): 563–571. ISSN 0950-382X. PMID 9489668.
  11. ↑ Stumpe, S.; Schlösser, A.; Schleyer, M.; Bakker, E. P. (1996-01-01). W.N. Konings, H. R. Kaback and J. S. Lolkema, ed. Chapter 21 K+ circulation across the prokaryotic cell membrane: K+-uptake systems. Transport Processes in Eukaryotic and Prokaryotic Organisms 2. North-Holland. pp. 473–499. doi:10.1016/s1383-8121(96)80062-5.
  12. ↑ Fujisawa, Makoto; Ito, Masahiro; Krulwich, Terry A. (2007-08-14). "Three two-component transporters with channel-like properties have monovalent cation/proton antiport activity". Proceedings of the National Academy of Sciences of the United States of America 104 (33): 13289–13294. doi:10.1073/pnas.0703709104. ISSN 0027-8424. PMC 1948933. PMID 17679694.
  13. 1 2 Roosild, Tarmo P.; Castronovo, Samantha; Healy, Jess; Miller, Samantha; Pliotas, Christos; Rasmussen, Tim; Bartlett, Wendy; Conway, Stuart J.; Booth, Ian R. (2010-11-16). "Mechanism of ligand-gated potassium efflux in bacterial pathogens". Proceedings of the National Academy of Sciences of the United States of America 107 (46): 19784–19789. doi:10.1073/pnas.1012716107. ISSN 1091-6490. PMC 2993342. PMID 21041667.
  14. ↑ Nakamura, C.; Kikuchi, T.; Burgess, J. G.; Matsunaga, T. (1995-07-01). "Iron-regulated expression and membrane localization of the magA protein in Magnetospirillum sp. strain AMB-1". Journal of Biochemistry 118 (1): 23–27. ISSN 0021-924X. PMID 8537318.
  15. ↑ Uebe, René; Henn, Verena; Schüler, Dirk (2012-03-01). "The MagA protein of Magnetospirilla is not involved in bacterial magnetite biomineralization". Journal of Bacteriology 194 (5): 1018–1023. doi:10.1128/JB.06356-11. ISSN 1098-5530. PMC 3294778. PMID 22194451.
  16. ↑ Southworth, T. W.; Guffanti, A. A.; Moir, A.; Krulwich, T. A. (2001-10-01). "GerN, an endospore germination protein of Bacillus cereus, is an Na(+)/H(+)-K(+) antiporter". Journal of Bacteriology 183 (20): 5896–5903. doi:10.1128/JB.183.20.5896-5903.2001. ISSN 0021-9193. PMC 99667. PMID 11566988.
  17. ↑ Tani, K.; Watanabe, T.; Matsuda, H.; Nasu, M.; Kondo, M. (1996-01-01). "Cloning and sequencing of the spore germination gene of Bacillus megaterium ATCC 12872: similarities to the NaH-antiporter gene of Enterococcus hirae". Microbiology and Immunology 40 (2): 99–105. ISSN 0385-5600. PMID 8867604.

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