N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase

N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (EC 3.5.1.103, MshB) is an enzyme with systematic name 1-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol acetylhydrolase.^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

1-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H₂O

\rightleftharpoons

1-(2-amino-2-deoxy-alpha-D-glucopyranoside)-1D-myo-inositol + acetate

This enzyme mediates the rate limiting step in the mycothiol biosynthesis pathway.

References

↑ Rawat, M., Kovacevic, S., Billman-Jacobe, H. and Av-Gay, Y. (2003). "Inactivation of mshB, a key gene in the mycothiol biosynthesis pathway in Mycobacterium smegmatis". Microbiology 149: 1341–1349. doi:10.1099/mic.0.26084-0. PMID 12724395.
↑ Newton, G.L., Av-Gay, Y. and Fahey, R.C. (2000). "N-Acetyl-1-D-myo-inosityl-2-amino-2-deoxy-α-D-glucopyranoside deacetylase (MshB) is a key enzyme in mycothiol biosynthesis". J. Bacteriol. 182: 6958–6963. doi:10.1128/jb.182.24.6958-6963.2000. PMID 11092856.
↑ Maynes, J.T., Garen, C., Cherney, M.M., Newton, G., Arad, D., Av-Gay, Y., Fahey, R.C. and James, M.N. (2003). "The crystal structure of 1-D-myo-inosityl 2-acetamido-2-deoxy-α-D-glucopyranoside deacetylase (MshB) from Mycobacterium tuberculosis reveals a zinc hydrolase with a lactate dehydrogenase fold". J. Biol. Chem. 278: 47166–47170. doi:10.1074/jbc.m308914200. PMID 12958317.

External links

N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase at the US National Library of Medicine Medical Subject Headings (MeSH)

Hydrolases: carbon-nitrogen non-peptide (EC 3.5)

3.5.1: Linear amides / Amidohydrolases	Asparaginase Glutaminase Urease Biotinidase Aspartoacylase Ceramidase Aspartylglucosaminidase Fatty acid amide hydrolase Histone deacetylase Sirtuin

3.5.2: Cyclic amides/ Amidohydrolases	Barbiturase Beta-lactamase

3.5.3: Linear amidines/ Ureohydrolases	Arginase Agmatinase Protein-arginine deiminase

3.5.4: Cyclic amidines/ Aminohydrolases	Guanine deaminase Adenosine deaminase AMP deaminase Inosine monophosphate synthase DCMP deaminase GTP cyclohydrolase I Cytidine deaminase AICDA Activation-induced cytidine deaminase

3.5.5: Nitriles/ Aminohydrolases	Nitrilase

3.5.99: Other	Riboflavinase Thiaminase II

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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