N-substituted formamide deformylase

N-substituted formamide deformylase
Identifiers
EC number	3.5.1.91
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

In enzymology, a N-substituted formamide deformylase (EC 3.5.1.91) is an enzyme that catalyzes the chemical reaction

N-benzylformamide + H₂O $\rightleftharpoons$ formate + benzylamine

Thus, the two substrates of this enzyme are N-benzylformamide and H₂O, whereas its two products are formate and benzylamine.^[1]

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-benzylformamide amidohydrolase and is also called NfdA. The enzyme is produced by Arthrobacter pascens bacteria.^[2]

References

1. Fukatsu, H.; Hashimoto, Y.; Goda, M.; Higashibata, H.; Kobayashi, M. (2004). "Amine-synthesizing enzyme N-substituted formamide deformylase: screening, purification, characterization, and gene cloning". Proc. Natl. Acad. Sci. 101 (38): 13726–13731. doi:10.1073/pnas.0405082101. PMID 15358859.
2. Schomburg, D.; Schomburg, I.; Chang, A., eds. (2009). "3.5.1.91 N-substituted formamide deformylase". Class 3 Hydrolases: EC 3.4.22-3.13. Springer Handbook of Enzymes (2nd ed.). Springer Science & Business Media. pp. 376–378. ISBN 9783540857051.