N2-citryl-N6-acetyl-N6-hydroxylysine synthase

N²-citryl-N⁶-acetyl-N⁶-hydroxylysine synthase (EC 6.3.2.38, N(alpha)-citryl-N(epsilon)-acetyl-N(epsilon)-hydroxylysine synthase, iucA (gene)) is an enzyme with systematic name citrate:N⁶-acetyl-N⁶-hydroxy-L-lysine ligase (ADP-forming).^[1]^[2]^[3]^[4]^[5] This enzyme catalyses the following chemical reaction

2 ATP + citrate + N⁶-acetyl-N⁶-hydroxy-L-lysine + H₂O

\rightleftharpoons

2 ADP + 2 phosphate + N²-citryl-N⁶-acetyl-N⁶-hydroxy-L-lysine

This enzyme requires Mg²⁺.

References

↑ Appanna, D.L., Grundy, B.J., Szczepan, E.W. and Viswanatha, T. (1984). "Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1". Biochim. Biophys. Acta 801: 437–443. doi:10.1016/0304-4165(84)90150-8.
↑ Gibson, F. and Magrath, D.I. (1969). "The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I". Biochim. Biophys. Acta 192 (2): 175–184. doi:10.1016/0304-4165(69)90353-5. PMID 4313071.
↑ Maurer, P.J. and Miller, M. (1982). "Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N⁶-acetyl-N⁶-hydroxylysine". J. Am. Chem. Soc. 104: 3096–3101. doi:10.1021/ja00375a025.
↑ de Lorenzo, V., Bindereif, A., Paw, B.H. and Neilands, J.B. (1986). "Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12". J. Bacteriol. 165 (2): 570–578. PMC 214457. PMID 2935523.
↑ Challis, G.L. (2005). "A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases". ChemBioChem 6: 601–611. doi:10.1002/cbic.200400283. PMID 15719346.

External links

N2-citryl-N6-acetyl-N6-hydroxylysine synthase at the US National Library of Medicine Medical Subject Headings (MeSH)

Enzymes: CO CS and CN ligases (EC 6.1-6.3)

6.1: Carbon-Oxygen	Aminoacyl tRNA synthetase Alanine Arginine Asparagine Aspartate Cysteine D-alanine—poly(phosphoribitol) ligase Glutamate Glutamine Glycine Histidine Isoleucine Leucine Lysine Methionine Phenylalanine Proline Serine Threonine Tryptophan Tyrosine Valine

6.2: Carbon-Sulfur	Succinyl coenzyme A synthetase Acetyl—CoA synthetase Long-chain-fatty-acid—CoA ligase

6.3: Carbon-Nitrogen	Glutamine synthetase Ubiquitin ligase Cullin Von Hippel–Lindau tumor suppressor UBE3A Mdm2 Anaphase-promoting complex UBR1 Glutathione synthetase CTP synthetase Adenylosuccinate synthase Argininosuccinate synthase Holocarboxylase synthetase GMP synthase Asparagine synthetase Carbamoyl phosphate synthetase I II Glutamate–cysteine ligase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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