NONO (protein)

Non-POU domain containing, octamer-binding
PDB rendering based on 2cpj.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3SDE​
Identifiers
Symbols	NONO ; NMT55; NRB54; P54; P54NRB; PPP1R114
External IDs	OMIM: 300084 MGI: 1855692 HomoloGene: 7212 GeneCards: NONO Gene
Gene ontology Molecular function • nucleotide binding • RNA polymerase II distal enhancer sequence-specific DNA binding • core promoter binding • chromatin binding • protein binding • identical protein binding • poly(A) RNA binding Cellular component • nucleus • nucleoplasm • nucleolus • membrane • nuclear matrix • nuclear speck • paraspeckles • RNA polymerase II transcription factor complex Biological process • DNA repair • DNA recombination • transcription, DNA-templated • mRNA processing • circadian rhythm • RNA splicing • regulation of circadian rhythm • negative regulation of transcription, DNA-templated • negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4841	53610
Ensembl	ENSG00000147140	ENSMUSG00000031311
UniProt	Q15233	Q99K48
RefSeq (mRNA)	NM_001145408	NM_001252518
RefSeq (protein)	NP_001138880	NP_001239447
Location (UCSC)	Chr X: 71.28 – 71.3 Mb	Chr X: 101.43 – 101.45 Mb
PubMed search

Non-POU domain-containing octamer-binding protein is a protein that in humans is encoded by the NONO gene.^[1][2][3]

Interactions

NONO has been shown to interact with SFPQ,^[4] SPI1^[5] and Androgen receptor.^[6]

References

1. Dong B, Horowitz DS, Kobayashi R, Krainer AR (Oct 1993). "Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6". Nucleic Acids Res. 21 (17): 4085–92. doi:10.1093/nar/21.17.4085. PMC 310009. PMID 8371983.
2. Traish AM, Huang YH, Ashba J, Pronovost M, Pavao M, McAneny DB, Moreland RB (Dec 1997). "Loss of expression of a 55 kDa nuclear protein (nmt55) in estrogen receptor-negative human breast cancer". Diagn. Mol. Pathol. 6 (4): 209–21. doi:10.1097/00019606-199708000-00005. PMID 9360842.
3. "Entrez Gene: NONO Non-POU domain containing, octamer-binding".
4. Peng R, Dye BT, Pérez I, Barnard DC, Thompson AB, Patton JG (Oct 2002). "PSF and p54nrb bind a conserved stem in U5 snRNA". RNA 8 (10): 1334–47. doi:10.1017/S1355838202022070. PMC 1370341. PMID 12403470.
5. Hallier M, Tavitian A, Moreau-Gachelin F (May 1996). "The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb". J. Biol. Chem. 271 (19): 11177–81. doi:10.1074/jbc.271.19.11177. PMID 8626664.
6. Ishitani K, Yoshida T, Kitagawa H, Ohta H, Nozawa S, Kato S (Jul 2003). "p54nrb acts as a transcriptional coactivator for activation function 1 of the human androgen receptor". Biochem. Biophys. Res. Commun. 306 (3): 660–5. doi:10.1016/S0006-291X(03)01021-0. PMID 12810069.

Further reading

• Shav-Tal Y, Zipori D (2002). "PSF and p54(nrb)/NonO--multi-functional nuclear proteins". FEBS Lett. 531 (2): 109–14. doi:10.1016/S0014-5793(02)03447-6. PMID 12417296.
• Zhang WW, Zhang LX, Busch RK, Farrés J, Busch H (1993). "Purification and characterization of a DNA-binding heterodimer of 52 and 100 kDa from HeLa cells". Biochem. J. 290 ( Pt 1) (Pt 1): 267–72. PMC 1132410. PMID 8439294.
• Hallier M, Tavitian A, Moreau-Gachelin F (1996). "The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb". J. Biol. Chem. 271 (19): 11177–81. doi:10.1074/jbc.271.19.11177. PMID 8626664.
• Peters U, Haberhausen G, Kostrzewa M, Nolte D, Müller U (1997). "AFX1 and p54nrb: fine mapping, genomic structure, and exclusion as candidate genes of X-linked dystonia parkinsonism". Hum. Genet. 100 (5-6): 569–72. doi:10.1007/s004390050553. PMID 9341872.
• Clark J, Lu YJ, Sidhar SK, Parker C, Gill S, Smedley D, Hamoudi R, Linehan WM, Shipley J, Cooper CS (1997). "Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma". Oncogene 15 (18): 2233–9. doi:10.1038/sj.onc.1201394. PMID 9393982.
• Straub T, Grue P, Uhse A, Lisby M, Knudsen BR, Tange TO, Westergaard O, Boege F (1998). "The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction". J. Biol. Chem. 273 (41): 26261–4. doi:10.1074/jbc.273.41.26261. PMID 9756848.
• Karhumaa P, Parkkila S, Waheed A, Parkkila AK, Kaunisto K, Tucker PW, Huang CJ, Sly WS, Rajaniemi H (2000). "Nuclear NonO/p54(nrb) protein is a nonclassical carbonic anhydrase". J. Biol. Chem. 275 (21): 16044–9. doi:10.1074/jbc.275.21.16044. PMID 10821857.
• Straub T, Knudsen BR, Boege F (2000). "PSF/p54(nrb) stimulates "jumping" of DNA topoisomerase I between separate DNA helices". Biochemistry 39 (25): 7552–8. doi:10.1021/bi992898e. PMID 10858305.
• Mathur M, Tucker PW, Samuels HH (2001). "PSF is a novel corepressor that mediates its effect through Sin3A and the DNA binding domain of nuclear hormone receptors". Mol. Cell. Biol. 21 (7): 2298–311. doi:10.1128/MCB.21.7.2298-2311.2001. PMC 86864. PMID 11259580.
• Zhang Z, Carmichael GG (2001). "The fate of dsRNA in the nucleus: a p54(nrb)-containing complex mediates the nuclear retention of promiscuously A-to-I edited RNAs". Cell 106 (4): 465–75. doi:10.1016/S0092-8674(01)00466-4. PMID 11525732.
• Pavao M, Huang YH, Hafer LJ, Moreland RB, Traish AM (2001). "Immunodetection of nmt55/p54nrb isoforms in human breast cancer". BMC Cancer 1: 15. doi:10.1186/1471-2407-1-15. PMC 59838. PMID 11710964.
• Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298.
• Sewer MB, Nguyen VQ, Huang CJ, Tucker PW, Kagawa N, Waterman MR (2002). "Transcriptional activation of human CYP17 in H295R adrenocortical cells depends on complex formation among p54(nrb)/NonO, protein-associated splicing factor, and SF-1, a complex that also participates in repression of transcription". Endocrinology 143 (4): 1280–90. doi:10.1210/en.143.4.1280. PMID 11897684.
• Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929.
• Emili A, Shales M, McCracken S, Xie W, Tucker PW, Kobayashi R, Blencowe BJ, Ingles CJ (2002). "Splicing and transcription-associated proteins PSF and p54nrb/nonO bind to the RNA polymerase II CTD". RNA 8 (9): 1102–11. doi:10.1017/S1355838202025037. PMC 1370324. PMID 12358429.
• Peng R, Dye BT, Pérez I, Barnard DC, Thompson AB, Patton JG (2002). "PSF and p54nrb bind a conserved stem in U5 snRNA". RNA 8 (10): 1334–47. doi:10.1017/S1355838202022070. PMC 1370341. PMID 12403470.