Nucleoporin 50

Nucleoporin 50kDa
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2EC1​, 3TJ3​
Identifiers
Symbols	NUP50 ; NPAP60; NPAP60L
External IDs	OMIM: 604646 MGI: 1351502 HomoloGene: 5190 GeneCards: NUP50 Gene
Gene ontology Molecular function • protein binding Cellular component • nuclear pore • nucleoplasm • cytoplasm • nuclear membrane Biological process • mitotic cell cycle • carbohydrate metabolic process • mitotic nuclear envelope disassembly • hexose transport • gene expression • regulation of glucose transport • protein transport • glucose transport • viral process • protein sumoylation • viral life cycle • viral transcription • cytokine-mediated signaling pathway • cellular response to heat • post-translational protein modification • cellular protein metabolic process • small molecule metabolic process • intracellular transport • mRNA transport • transmembrane transport • regulation of cellular response to heat Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	10762	18141
Ensembl	ENSG00000093000	ENSMUSG00000016619
UniProt	Q9UKX7	Q9JIH2
RefSeq (mRNA)	NM_007172	NM_016714
RefSeq (protein)	NP_009103	NP_057923
Location (UCSC)	Chr 22: 45.16 – 45.19 Mb	Chr 15: 84.92 – 84.94 Mb
PubMed search

Nucleoporin 50 (Nup50) is a protein that in humans is encoded by the NUP50 gene.^[1][2]

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.^[2]

Interactions

NUP50 has been shown to interact with KPNB1^[3] and CDKN1B.^[4]

References

1. Trichet V, Shkolny D, Dunham I, Beare D, McDermid HE (October 1999). "Mapping and complex expression pattern of the human NPAP60L nucleoporin gene". Cytogenet Cell Genet 85 (3–4): 221–6. doi:10.1159/000015297. PMID 10449902.
2. "Entrez Gene: NUP50 nucleoporin 50kDa".
3. Lindsay, Mark E; Plafker Kendra; Smith Alicia E; Clurman Bruce E; Macara Ian G (August 2002). "Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import". Cell (United States) 110 (3): 349–60. doi:10.1016/S0092-8674(02)00836-X. ISSN 0092-8674. PMID 12176322.
4. Smitherman, M; Lee K; Swanger J; Kapur R; Clurman B E (August 2000). "Characterization and Targeted Disruption of Murine Nup50, a p27Kip1-Interacting Component of the Nuclear Pore Complex". Mol. Cell. Biol. (UNITED STATES) 20 (15): 5631–42. doi:10.1128/MCB.20.15.5631-5642.2000. ISSN 0270-7306. PMC 86029. PMID 10891500.

Further reading

• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
• Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Collins JE, Wright CL, Edwards CA, et al. (2005). "A genome annotation-driven approach to cloning the human ORFeome". Genome Biol. 5 (10): R84. doi:10.1186/gb-2004-5-10-r84. PMC 545604. PMID 15461802.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Swaminathan S, Melchior F (2002). "Nucleocytoplasmic transport: more than the usual suspects". Dev. Cell 3 (3): 304–6. doi:10.1016/S1534-5807(02)00262-9. PMID 12361592.
• Lindsay ME, Plafker K, Smith AE, et al. (2002). "Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import". Cell 110 (3): 349–60. doi:10.1016/S0092-8674(02)00836-X. PMID 12176322.
• Smitherman M, Lee K, Swanger J, et al. (2000). "Characterization and Targeted Disruption of Murine Nup50, a p27Kip1-Interacting Component of the Nuclear Pore Complex". Mol. Cell. Biol. 20 (15): 5631–42. doi:10.1128/MCB.20.15.5631-5642.2000. PMC 86029. PMID 10891500.
• Guan T, Kehlenbach RH, Schirmer EC, et al. (2000). "Nup50, a Nucleoplasmically Oriented Nucleoporin with a Role in Nuclear Protein Export". Mol. Cell. Biol. 20 (15): 5619–30. doi:10.1128/MCB.20.15.5619-5630.2000. PMC 86026. PMID 10891499.
• Dunham I, Shimizu N, Roe BA, et al. (1999). "The DNA sequence of human chromosome 22". Nature 402 (6761): 489–95. doi:10.1038/990031. PMID 10591208.