Nucleoporin 85
Nucleoporin 85 (Nup85) is a protein that in humans is encoded by the NUP85 gene.[1][2]
Function
Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. NPCs are composed of subcomplexes, and NUP85 is part of one such subcomplex, Nup107-160.[2]
Model organisms
Model organisms have been used in the study of NUP85 function. A conditional knockout mouse line called Nup85tm1a(KOMP)Wtsi was generated at the Wellcome Trust Sanger Institute.[3] Male and female animals underwent a standardized phenotypic screen[4] to determine the effects of deletion.[5][6][7][8] Additional screens performed: - In-depth immunological phenotyping[9]
| Characteristic | Phenotype | |
|---|---|---|
| All data available at.[4][9] | Insulin | Normal |
| Homozygous viability at P14 | Abnormal | |
| Recessive lethal study | Abnormal | |
| Body weight | Normal | |
| Neurological assessment | Normal | |
| Grip strength | Normal | |
| Dysmorphology | Normal | |
| Indirect calorimetry | Normal | |
| Glucose tolerance test | Normal | |
| Auditory brainstem response | Normal | |
| DEXA | Normal | |
| Radiography | Normal | |
| Eye morphology | Normal | |
| Clinical chemistry | Normal | |
| Haematology 16 Weeks | Normal | |
| Peripheral blood leukocytes 16 Weeks | Normal | |
| Heart weight | Normal | |
| Salmonella infection | Normal | |
| Cytotoxic T Cell Function | Normal | |
| Anti-nuclear Antibody Assay | Normal | |
| Epidermal Immune Composition | Normal | |
| Influenza Challenge | Normal | |
References
- ↑ Doxsey SJ, Stein P, Evans L, Calarco PD, Kirschner M (Feb 1994). "Pericentrin, a highly conserved centrosome protein involved in microtubule organization". Cell 76 (4): 639–50. doi:10.1016/0092-8674(94)90504-5. PMID 8124707.
- 1 2 "Entrez Gene: NUP85 nucleoporin 85kDa".
- ↑ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
- 1 2 "International Mouse Phenotyping Consortium".
- ↑ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- ↑ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- ↑ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
- ↑ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
- 1 2 "Infection and Immunity Immunophenotyping (3i) Consortium".
Further reading
- Kapeller R, Toker A, Cantley LC, Carpenter CL (Oct 1995). "Phosphoinositide 3-kinase binds constitutively to alpha/beta-tubulin and binds to gamma-tubulin in response to insulin". The Journal of Biological Chemistry 270 (43): 25985–91. doi:10.1074/jbc.270.43.25985. PMID 7592789.
- Tynan SH, Purohit A, Doxsey SJ, Vallee RB (Oct 2000). "Light intermediate chain 1 defines a functional subfraction of cytoplasmic dynein which binds to pericentrin". The Journal of Biological Chemistry 275 (42): 32763–8. doi:10.1074/jbc.M001536200. PMID 10893222.
- Cronshaw JM, Krutchinsky AN, Zhang W, Chait BT, Matunis MJ (Sep 2002). "Proteomic analysis of the mammalian nuclear pore complex". The Journal of Cell Biology 158 (5): 915–27. doi:10.1083/jcb.200206106. PMC 2173148. PMID 12196509.
- Mulari MT, Patrikainen L, Kaisto T, Metsikkö K, Salo JJ, Väänänen HK (May 2003). "The architecture of microtubular network and Golgi orientation in osteoclasts--major differences between avian and mammalian species". Experimental Cell Research 285 (2): 221–35. doi:10.1016/S0014-4827(03)00033-8. PMID 12706117.
- Chang F, Re F, Sebastian S, Sazer S, Luban J (Apr 2004). "HIV-1 Vpr induces defects in mitosis, cytokinesis, nuclear structure, and centrosomes". Molecular Biology of the Cell 15 (4): 1793–801. doi:10.1091/mbc.E03-09-0691. PMC 379276. PMID 14767062.
- Loïodice I, Alves A, Rabut G, Van Overbeek M, Ellenberg J, Sibarita JB, Doye V (Jul 2004). "The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis". Molecular Biology of the Cell 15 (7): 3333–44. doi:10.1091/mbc.E03-12-0878. PMC 452587. PMID 15146057.
- Terashima Y, Onai N, Murai M, Enomoto M, Poonpiriya V, Hamada T, Motomura K, Suwa M, Ezaki T, Haga T, Kanegasaki S, Matsushima K (Aug 2005). "Pivotal function for cytoplasmic protein FROUNT in CCR2-mediated monocyte chemotaxis". Nature Immunology 6 (8): 827–35. doi:10.1038/ni1222. PMID 15995708.
- Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
- Satoh M, Akatsu T, Ishkawa Y, Minami Y, Nakamura M (Mar 2007). "A novel activator of C-C chemokine, FROUNT, is expressed with C-C chemokine receptor 2 and its ligand in failing human heart". Journal of Cardiac Failure 13 (2): 114–9. doi:10.1016/j.cardfail.2006.11.003. PMID 17395051.
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