PUM2

Pumilio RNA-binding family member 2
PDB rendering based on 1ib2.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3Q0Q​, 3Q0R​, 3Q0S​
Identifiers
Symbols	PUM2 ; PUMH2; PUML2
External IDs	OMIM: 607205 MGI: 1931751 HomoloGene: 69183 GeneCards: PUM2 Gene
Gene ontology Molecular function • mRNA 3'-UTR binding • protein binding • poly(A) RNA binding Cellular component • cytoplasm • cytoplasmic stress granule • nuclear membrane • perinuclear region of cytoplasm Biological process • regulation of translation • stress granule assembly • regulation of gene silencing by miRNA • positive regulation of RIG-I signaling pathway • positive regulation of gene silencing by miRNA Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	23369	80913
Ensembl	ENSG00000055917	ENSMUSG00000020594
UniProt	Q8TB72	Q80U58
RefSeq (mRNA)	NM_001282752	NM_001160219
RefSeq (protein)	NP_001269681	NP_001153691
Location (UCSC)	Chr 2: 20.25 – 20.35 Mb	Chr 12: 8.67 – 8.75 Mb
PubMed search

Pumilio homolog 2 is an RNA-binding protein that in humans is encoded by the PUM2 gene.^[1][2][3][4]

Interactions

BRCA1 has been shown to interact with the following proteins:

• CPEB^[5]
• DAZL^[6]
• DAZ1^[7]
• NANOS1^[8]

References

1. Nagase T, Seki N, Ishikawa K, Ohira M, Kawarabayasi Y, Ohara O, Tanaka A, Kotani H, Miyajima N, Nomura N (May 1997). "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain". DNA Res 3 (5): 321–9, 341–54. doi:10.1093/dnares/3.5.321. PMID 9039502.
2. Spassov DS, Jurecic R (Dec 2002). "Cloning and comparative sequence analysis of PUM1 and PUM2 genes, human members of the Pumilio family of RNA-binding proteins". Gene 299 (1–2): 195–204. doi:10.1016/S0378-1119(02)01060-0. PMID 12459267.
3. Moore FL, Jaruzelska J, Fox MS, Urano J, Firpo MT, Turek PJ, Dorfman DM, Pera RA (Jan 2003). "Human Pumilio-2 is expressed in embryonic stem cells and germ cells and interacts with DAZ (Deleted in AZoospermia) and DAZ-Like proteins". Proc Natl Acad Sci U S A 100 (2): 538–43. doi:10.1073/pnas.0234478100. PMC 141031. PMID 12511597.
4. "Entrez Gene: PUM2 pumilio homolog 2 (Drosophila)".
5. Campbell ZT, Menichelli E, Friend K, Wu J, Kimble J, Williamson JR, Wickens M (2012). "Identification of a conserved interface between PUF and CPEB proteins.". J Biol Chem 287 (22): 18854–62. doi:10.1074/jbc.M112.352815. PMID 22496444.
6. Ota R, Kotani T, Yamashita M (2011). "Biochemical characterization of Pumilio1 and Pumilio2 in Xenopus oocytes.". J Biol Chem 286 (4): 2853–63. doi:10.1074/jbc.M110.155523. PMID 21098481.
7. Haston KM, Tung JY, Reijo Pera RA (2009). "Dazl functions in maintenance of pluripotency and genetic and epigenetic programs of differentiation in mouse primordial germ cells in vivo and in vitro.". PLOS ONE 4 (5): e5654. doi:10.1371/journal.pone.0005654. PMID 19468308.
8. Geyer CB, Saba R, Kato Y, Anderson AJ, Chappell VK, Saga Y, Eddy EM (2012). "Rhox13 is translated in premeiotic germ cells in male and female mice and is regulated by NANOS2 in the male.". Biol Reprod 86 (4): 127. doi:10.1095/biolreprod.111.094938. PMID 22190708.

Further reading

• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (2003). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Res. 9 (3): 99–106. doi:10.1093/dnares/9.3.99. PMID 12168954.
• Spik A, Oczkowski S, Olszak A, Kotecki M, Formanowicz P, Błazewicz J, Jaruzelska J (2006). "Candidate mRNAs interacting with fertility protein PUMILIO2 in the human germ line". Reproductive biology. 6 Suppl 1: 37–42. PMID 16967088.
• Nakayama M, Kikuno R, Ohara O (2003). "Protein–Protein Interactions Between Large Proteins: Two-Hybrid Screening Using a Functionally Classified Library Composed of Long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
• Jaruzelska J, Kotecki M, Kusz K, Spik A, Firpo M, Reijo Pera RA (2004). "Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells". Dev. Genes Evol. 213 (3): 120–6. doi:10.1007/s00427-003-0303-2. PMID 12690449.
• Dazard JE, Gal H, Amariglio N, Rechavi G, Domany E, Givol D (2003). "Genome-wide comparison of human keratinocyte and squamous cell carcinoma responses to UVB irradiation: implications for skin and epithelial cancer". Oncogene 22 (19): 2993–3006. doi:10.1038/sj.onc.1206537. PMID 12771951.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Fox M, Urano J, Reijo Pera RA (2005). "Identification and characterization of RNA sequences to which human PUMILIO-2 (PUM2) and deleted in Azoospermia-like (DAZL) bind". Genomics 85 (1): 92–105. doi:10.1016/j.ygeno.2004.10.003. PMID 15607425.
• Scott RE, White-Grindley E, Ruley HE, Chesler EJ, Williams RW (2005). "P2P-R expression is genetically coregulated with components of the translation machinery and with PUM2, a translational repressor that associates with the P2P-R mRNA". J. Cell. Physiol. 204 (1): 99–105. doi:10.1002/jcp.20263. PMID 15617101.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
• Kusz K, Ginter-Matuszewska B, Ziolkowska K, Spik A, Bierla J, Jedrzejczak P, Latos-Bielenska A, Pawelczyk L, Jaruzelska J (2007). "Polymorphisms of the human PUMILIO2 gene and male sterility". Mol. Reprod. Dev. 74 (6): 795–9. doi:10.1002/mrd.20683. PMID 17154300.