Peroxyureidoacrylate/ureidoacrylate amidohydrolase

Peroxyureidoacrylate/ureidoacrylate amidohydrolase (EC 3.5.1.110, RutB) is an enzyme with systematic name (Z)-3-ureidoacrylate peracid amidohydrolase.^[1] This enzyme catalyses the following chemical reaction

(1) (Z)-3-ureidoacrylate peracid + H₂O

\rightleftharpoons

(Z)-3-peroxyaminoacrylate + CO₂ + NH₃ (overall reaction)

(1a) (Z)-3-ureidoacrylate peracid + H₂O

\rightleftharpoons

(Z)-3-peroxyaminoacrylate + carbamate

(1b) carbamate

\rightleftharpoons

CO₂ + NH₃ (spontaneous)

(2) (Z)-2-methylureidoacrylate peracid + H₂O

\rightleftharpoons

(Z)-2-methylperoxyaminoacrylate + CO₂ + NH₃ (overall reaction)

(2a) (Z)-2-methylureidoacrylate peracid + H₂O

\rightleftharpoons

(Z)-2-methylperoxyaminoacrylate + carbamate

(2b) carbamate

\rightleftharpoons

CO₂ + NH₃ (spontaneous)

The enzyme also shows activity towards ureidoacrylate.

References

↑ Kim, K.S., Pelton, J.G., Inwood, W.B., Andersen, U., Kustu, S. and Wemmer, D.E. (2010). "The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems". J. Bacteriol. 192: 4089–4102. doi:10.1128/JB.00201-10. PMC 2916427. PMID 20400551.

External links

Peroxyureidoacrylate/ureidoacrylate amidohydrolase at the US National Library of Medicine Medical Subject Headings (MeSH)

Hydrolases: carbon-nitrogen non-peptide (EC 3.5)

3.5.1: Linear amides / Amidohydrolases	Asparaginase Glutaminase Urease Biotinidase Aspartoacylase Ceramidase Aspartylglucosaminidase Fatty acid amide hydrolase Histone deacetylase Sirtuin

3.5.2: Cyclic amides/ Amidohydrolases	Barbiturase Beta-lactamase

3.5.3: Linear amidines/ Ureohydrolases	Arginase Agmatinase Protein-arginine deiminase

3.5.4: Cyclic amidines/ Aminohydrolases	Guanine deaminase Adenosine deaminase AMP deaminase Inosine monophosphate synthase DCMP deaminase GTP cyclohydrolase I Cytidine deaminase AICDA Activation-induced cytidine deaminase

3.5.5: Nitriles/ Aminohydrolases	Nitrilase

3.5.99: Other	Riboflavinase Thiaminase II

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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