Plasmodesma

Diagram of some plasmodesmata
Plasmodesmata allow molecules to travel between plant cells through the symplastic pathway
The structure of a primary plasmodesma. CW=Cell wall CA=Callose PM=Plasma membrane ER=Endoplasmic reticulum DM=Desmotubule Red circles=Actin Purple circles and spokes=Other unidentified proteins[1]

Plasmodesmata (singular: plasmodesma) are microscopic channels which traverse the cell walls of plant cells[2] and some algal cells, enabling transport and communication between them. Plasmodesmata evolved independently in several lineages,[3] and species that have these structures include members of the Charophyceae, Charales, Coleochaetales and Phaeophyceae (which are all algae), as well as all embryophytes, better known as land plants.[4] Unlike animal cells, almost every plant cell is surrounded by a polysaccharide cell wall. Neighbouring plant cells are therefore separated by a pair of cell walls and the intervening middle lamella, forming an extracellular domain known as the apoplast. Although cell walls are permeable to small soluble proteins and other solutes, plasmodesmata enable direct, regulated, symplastic intercellular transport of substances between cells. There are two forms of plasmodesmata: primary plasmodesmata, which are formed during cell division, and secondary plasmodesmata, which can form between mature cells.[5]

Similar structures, called gap junctions[6] and membrane nanotubes, interconnect animal cells[7] and stromules form between plastids in plant cells.[8]

Formation

Primary plasmodesmata are formed when portions of the endoplasmic reticulum are trapped across the middle lamella as new cell wall is laid down between two newly divided plant cells and these eventually become the cytoplasmic connections between cells. Here the wall is not thickened further, and depressions or thin areas known as pits are formed in the walls. Pits normally pair up between adjacent cells. Plasmodesmata can also be inserted into existing cell walls between non-dividing cells (secondary plasmodesmata)[9]

Structure

Plasmodesmatal plasma membrane

A typical plant cell may have between 103 and 105 plasmodesmata connecting it with adjacent cells[10] equating to between 1 and 10 per µm2.[11] Plasmodesmata are approximately 50-60 nm in diameter at the midpoint and are constructed of three main layers, the plasma membrane, the cytoplasmic sleeve, and the desmotubule.[10] They can transverse cell walls that are up to 90 nm thick.[11]

The plasma membrane portion of the plasmodesma is a continuous extension of the cell membrane or plasmalemma and has a similar phospholipid bilayer structure.[12]

Cytoplasmic sleeve

The cytoplasmic sleeve is a fluid-filled space enclosed by the plasmalemma and is a continuous extension of the cytosol. Trafficking of molecules and ions through plasmodesmata occurs through this space. Smaller molecules (e.g. sugars and amino acids) and ions can easily pass through plasmodesmata by diffusion without the need for additional chemical energy. Larger molecules, including proteins (for example Green fluorescent protein) and RNA, can also pass through the cytoplasmic sleeve diffusively.[13] Plasmodesmatal transport of some larger molecules is facilitated by mechanisms that are currently unknown. One mechanism of regulation of the permeability of plasmodesmata is the accumulation of the polysaccharide callose around the neck region to form a collar, thereby reducing the diameter of the pore available for transport of substances.[12]

Desmotubule

The desmotubule is a tube of appressed (flattened) endoplasmic reticulum that runs between two adjacent cells [14] Some molecules are known to be transported through this channel,[15] but it is not thought to be the main route for plasmodesmatal transport.

Around the desmotubule and the plasma membrane areas of an electron dense material have been seen, often joined together by spoke-like structures that seem to split the plasmodesma into smaller channels [14] These structures may be composed of myosin[16][17][18] and actin,[17][19] which are part of the cell's cytoskeleton. If this is the case these proteins could be used in the selective transport of large molecules between the two cells.

Transport

Tobacco mosaic virus movement protein 30 localizes to plasmodesmata.

Plasmodesmata have been shown to transport proteins (including transcription factors), short interfering RNA, messenger RNA and viral genomes from cell to cell. One example of a viral movement proteins is the tobacco mosaic virus MP-30. MP-30 is thought to bind to the virus's own genome and shuttle it from infected cells to uninfected cells through plasmodesmata.[13] Flowering Locus T protein moves from leaves to the shoot apical meristem through plasmodesmata to initiate flowering.[20]

Plasmodesmata are also used by cells in phloem, and symplastic transport is used to regulate the sieve-tube cells by the companion cells.[21]

The size of molecules that can pass through plasmodesmata is determined by the size exclusion limit. This limit is highly variable and is subject to active modification.[5] For example, MP-30 is able to increase the size exclusion limit from 700 Daltons to 9400 Daltons thereby aiding its movement through a plant.[22] Also, increasing calcium concentrations in the cytoplasm, either by injection or by cold-induction, has been shown to constrict the opening of surrounding plasmodesmata and limit transport. [23]

Several models for possible active transport through plasmodesmata exist. It has been suggested that such transport is mediated by interactions with proteins localized on the desmotubule, and/or by chaperones partially unfolding proteins, allowing them to fit through the narrow passage. A similar mechanism may be involved in transporting viral nucleic acids through the plasmodesmata.[24]

See also

References

  1. ↑ Maule, Andrew (December 2008). "Plasmodesmata: structure, function and biogenesis". Current Opinion in Plant Biology 11 (6): 680–686. doi:10.1016/j.pbi.2008.08.002. PMID 18824402.
  2. ↑ Oparka, K. J. (2005). Plasmodesmata. Blackwell Pub Professional. ISBN 978-1-4051-2554-3.
  3. ↑ Zoë A. Popper, Gurvan Michel, Cécile Hervé, David S. Domozych, William G.T. Willats, Maria G. Tuohy, Bernard Kloareg, Dagmar B. Stengel. "Evolution and Diversity of Plant Cell Walls: From Algae to Flowering Plants" (PDF). Annual Review of Plant Biology. doi:10.1146/annurev-arplant-042110-103809.
  4. ↑ Graham, LE; Cook, ME; Busse, JS (2000), Proceedings of the National Academy of Sciences 97, 4535-4540.
  5. 1 2 Jan Traas, Teva Vernoux (29 June 2002). "The shoot apical meristem: the dynamics of a stable structure". Philosophical Transactions of the Royal Society B: Biological Sciences 357 (1422): 737–747. PMC 1692983.
  6. ↑ Bruce Alberts (2002). Molecular biology of the cell (4th ed.). New York: Garland Science. ISBN 0-8153-3218-1.
  7. ↑ Gallagher KL, Benfey PN (15 January 2005). "Not just another hole in the wall: understanding intercellular protein trafficking". Genes & development 19 (2): 189–95. doi:10.1101/gad.1271005. PMID 15655108.
  8. ↑ Gray JC, Sullivan JA, Hibberd JM, Hansen MR (2001). "Stromules: mobile protrusions and interconnections between plastids". Plant Biology 3: 223–33. doi:10.1055/s-2001-15204.
  9. ↑ Lucas, W.; Ding, B.; Van der Schoot, C. (1993). "Tansley Review No.58 Plasmodesmata and the supracellular nature of plants". New Phytologist 125 (3): 435–476. doi:10.1111/j.1469-8137.1993.tb03897.x. JSTOR 2558257.
  10. 1 2 Robards, AW (1975). "Plasmodesmata". Annual Review of Plant Physiology 26: 13–29. doi:10.1146/annurev.pp.26.060175.000305.
  11. 1 2 Lodish, Berk, Zipursky, Matsudaira, Baltimore, Darnell (2000). "22". Molecular Cell Biology (4 ed.). p. 998. ISBN 0-7167-3706-X. OCLC 41266312.
  12. 1 2 AW Robards (1976). "Plasmodesmata in higher plants". In BES Gunning; AW Robards. Intercellular communications in plants: studies on plasmodesmata. Berlin: Springer-Verlag. pp. 15–57.
  13. 1 2 A. G. Roberts; K. J. Oparka (1 January 2003). "Plasmodesmata and the control of symplastic transport". Plant, Cell & Environment 26 (1): 103–124. doi:10.1046/j.1365-3040.2003.00950.x.
  14. 1 2 Overall, RL; Wolfe, J; Gunning, BES (1982). "Intercellular communication in Azolla roots: I. Ultrastructure of plasmodesmata". Protoplasma 111: 134–150. doi:10.1007/bf01282071.
  15. ↑ Cantrill, LC; Overall, RL; Goodwin, PB (1999). "Cell-to-cell communication via plant endomembranes". Cell Biology International 23: 653–661. doi:10.1006/cbir.1999.0431.
  16. ↑ Radford, JE; White, RG (1998). "Localization of a myosin‐like protein to plasmodesmata". Plant Journal 14: 743–750. doi:10.1046/j.1365-313x.1998.00162.x.
  17. 1 2 Blackman, LM; Overall, RL (1998). "Immunolocalisation of the cytoskeleton to plasmodesmata of Chara corallina". Plant Journal 14: 733–741. doi:10.1046/j.1365-313x.1998.00161.x.
  18. ↑ Reichelt, S; Knight, AE; Hodge, TP; Baluska, F; Samaj, J; Volkmann, D; Kendrick-Jones, J (1999). "Characterization of the unconventional myosin VIII in plant cells and its localization at the post-cytokinetic cell wall". Plant Journal 19: 555–569. doi:10.1046/j.1365-313x.1999.00553.x.
  19. ↑ White, RG; Badelt, K; Overall, RL; Vesk, M (1994). "Actin associated with plasmodesmata". Protoplasma 180: 169–184. doi:10.1007/bf01507853.
  20. ↑ Corbesier, L., Vincent, C., Jang, S., Fornara, F., Fan, Q.; et al. (2007). "FT protein movement contributes to long distance signalling in floral induction of Arabidopsis". Science 316 (5827): 1030–1033. doi:10.1126/science.1141752. PMID 17446353.
  21. ↑ Phloem
  22. ↑ Shmuel, Wolf; William, J. Lucas; Carl, M. Deom (1989). "Movement Protein of Tobacco Mosaic Virus Modifies Plasmodesmatal Size Exclusion Limit". Science 246 (4928): 377–379. doi:10.1126/science.246.4928.377.
  23. ↑ Aaziz, R.; Dinant, S.; Epel, B. L. (1 July 2001). "Plasmodesmata and plant cytoskeleton". Trends in Plant Science 6 (7): 326–330. ISSN 1360-1385. PMID 11435172.
  24. ↑ Plant Physiology lectures, chapter 5
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