PreQ1 riboswitch

PreQ1 riboswitch
Predicted secondary structure and sequence conservation of PreQ1
Identifiers
Symbol PreQ1
Rfam RF00522
Other data
RNA type Cis-reg; riboswitch
Domain(s) Bacteria
SO 0000035

The PreQ1-I riboswitch is a cis-acting element identified in bacteria which regulates expression of genes involved in biosynthesis of the nucleoside queuosine from GTP.[1] This RNA element known as a riboswitch binds preQ1 (pre-queuosine1), an intermediate in the queuosine pathway. Riboswitch function has been characterised in the bacterium Bacillus subtilis, where the riboswitch is located in the leader of the ykvJKLM (queCDEF) operon which encodes four genes necessary for queuosine production.[2] In this organism, PreQ1 binding to the riboswitch aptamer is thought to induce premature transcription termination within the leader to down-regulate expression of these genes. The preQ1 riboswitch is distinguished by its unusually small aptamer, compared to other riboswitches. Its atomic-resolution three-dimensional structure was determined, with the PDB ID 2L1V.[3][4] PreQ1-II riboswitches and preQ1-III riboswitches also bind preQ1, but have an unrelated, distinct structures.

References

  1. ↑ Roth A, Winkler WC, Regulski EE, Lee BW, Lim J, Jona I, Barrick JE, Ritwik A, Kim JN, Welz R, Iwata-Reuyl D, Breaker RR (2007). "A riboswitch selective for the queuosine precursor preQ1 contains an unusually small aptamer domain". Nat Struct Mol Biol 14 (4): 308–317. doi:10.1038/nsmb1224. PMID 17384645.
  2. ↑ Reader JS, Metzgar D, Schimmel P, de Crécy-Lagard V (2004). "Identification of four genes necessary for biosynthesis of the modified nucleoside queuosine". J. Biol. Chem. 279 (8): 6280–6285. doi:10.1074/jbc.M310858200. PMID 14660578.
  3. ↑ Klein DJ, Edwards TE, Ferré-D'Amaré AR (March 2009). "Cocrystal structure of a class I preQ1 riboswitch reveals a pseudoknot recognizing an essential hypermodified nucleobase". Nat. Struct. Mol. Biol. 16 (3): 343–344. doi:10.1038/nsmb.1563. PMC 2657927. PMID 19234468.
  4. ↑ Kang M, Peterson R, Feigon J (March 2009). "Structural Insights into riboswitch control of the biosynthesis of queuosine, a modified nucleotide found in the anticodon of tRNA". Mol. Cell 33 (6): 784–90. doi:10.1016/j.molcel.2009.02.019. PMID 19285444.

External links


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