PreQ1 synthase

PreQ1 synthase
Identifiers
EC number	1.7.1.13
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

In enzymology, a preQ1 synthase (EC 1.7.1.13) is an enzyme that catalyzes the chemical reaction

7-aminomethyl-7-carbaguanine + 2 NADP⁺ $\rightleftharpoons$ 7-cyano-7-carbaguanine + 2 NADPH + 2 H⁺

Thus, the two substrates of this enzyme are 7-aminomethyl-7-carbaguanine and NADP⁺, whereas its 3 products are 7-cyano-7-carbaguanine, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 7-aminomethyl-7-carbaguanine:NADP+ oxidoreductase. Other names in common use include YkvM, QueF, preQ0 reductase, preQ0 oxidoreductase, 7-cyano-7-deazaguanine reductase, 7-aminomethyl-7-carbaguanine:NADP+ oxidoreductase, queuine synthase (incorrect as queuine is not the product), queuine:NADP+ oxidoreductase (incorrect as queuine is not the, and product).

References

• Iwata-Reuyl D; Reader, JS; Swairjo, MA; De Crécy-Lagard, V; Lee, B; Iwata-Reuyl, D (2005). "From cyclohydrolase to oxidoreductase: Discovery of nitrile reductase activity in a common fold". Proc. Natl. Acad. Sci. U.S.A. 102 (12): 4264–9. doi:10.1073/pnas.0408056102. PMC 555470. PMID 15767583.
• Yokoyama S, Miyazawa T, Iitaka Y, Yamaizumi Z, Kasai H, Nishimura S (1979). "Three-dimensional structure of hyper-modified nucleoside Q located in the wobbling position of tRNA". Nature. 282 (5734): 107–9. doi:10.1038/282107a0. PMID 388227.
• Kuchino Y, Kasai H, Nihei K, Nishimura S (1976). "Biosynthesis of the modified nucleoside Q in transfer RNA". Nucleic. Acids. Res. 3 (2): 393–8. doi:10.1093/nar/3.2.393. PMC 342909. PMID 1257053.
• JA; Noguchi, S; Nishimura, S; Ohgi, T; Goto, T; Crain, PF; McCloskey, JA (1978). "Structure determination of a nucleoside Q precursor isolated from E. coli tRNA: 7-(aminomethyl)-7-deazaguanosine". Nucleic. Acids. Res. 5 (7): 2289–96. doi:10.1093/nar/5.7.2289. PMC 342163. PMID 353740.
• Nishimura S; Yamaizumi, Z; Ohgi, T; Goto, T; Nishimura, Y; Hirota, Y; Nishimura, S (1978). "Isolation of Q nucleoside precursor present in tRNA of an E. coli mutant and its characterization as 7-(cyano)-7-deazaguanosine". Nucleic. Acids. Res. 5 (11): 4215–23. doi:10.1093/nar/5.11.4215. PMC 342744. PMID 364423.
• Swairjo M.A., Reddy R.R., Lee B., Van Lanen S.G., Brown S., de Crécy-Lagard V., Iwata-Reuyl D, Schimmel P (2005). "Crystallization and preliminary X-ray characterization of the nitrile reductase QueF: a queuosine-biosynthesis enzyme". Acta Crystallogr. F 61 (Pt 10): 945–8. doi:10.1107/S1744309105029246. PMC 1991305. PMID 16511203.
• Chikwana, V.M., Stec, B., Lee, B.W.K., de Crécy-Lagard, V., Iwata-Reuyl, D. and Swairjo, M.A. (2012). "Structural basis of biological nitrile reduction.". J. Biological Chemistry 287:: 30560–70.