Pyrimidine oxygenase

Pyrimidine oxygenase
Identifiers
EC number	1.14.99.46
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Pyrimidine oxygenase (EC 1.14.99.46, RutA) is an enzyme with systematic name uracil,FMNH₂:oxygen oxidoreductase (uracil hydroxylating, ring-opening) .^[1][2] This enzyme catalyses the following chemical reaction

(1) uracil + FMNH₂ + O₂ $\rightleftharpoons$ (Z)-3-ureidoacrylate peracid + FMN

(2) thymine + FMNH₂ + O₂ $\rightleftharpoons$ (Z)-2-methylureidoacrylate peracid + FMN

In vitro the product (Z)-3-ureidoacrylate peracid is spontaneously reduced to ureidoacrylate.

References

1. Mukherjee, T., Zhang, Y., Abdelwahed, S., Ealick, S.E. and Begley, T.P. (2010). "Catalysis of a flavoenzyme-mediated amide hydrolysis". J. Am. Chem. Soc. 132 (16): 5550–5551. doi:10.1021/ja9107676. PMC 2873085. PMID 20369853.
2. Kim, K.S., Pelton, J.G., Inwood, W.B., Andersen, U., Kustu, S. and Wemmer, D.E. (2010). "The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems". J. Bacteriol. 192 (16): 4089–4102. doi:10.1128/JB.00201-10. PMC 2916427. PMID 20400551.

External links

• Pyrimidine oxygenase at the US National Library of Medicine Medical Subject Headings (MeSH)