RACGAP1

Rac GTPase activating protein 1
PDB rendering based on 2ovj.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2OVJ​, 3W6R​, 3WPQ​, 3WPS​, 4B6D​
Identifiers
Symbols	RACGAP1 ; CYK4; HsCYK-4; ID-GAP; MgcRacGAP
External IDs	OMIM: 604980 MGI: 1349423 HomoloGene: 8077 GeneCards: RACGAP1 Gene
Gene ontology Molecular function • GTPase activator activity • protein binding • phosphatidylinositol-3,4,5-trisphosphate binding • microtubule binding • protein kinase binding • alpha-tubulin binding • gamma-tubulin binding • metal ion binding • beta-tubulin binding Cellular component • acrosomal vesicle • nucleus • nucleoplasm • cytosol • microtubule • midbody • extrinsic component of cytoplasmic side of plasma membrane • cleavage furrow • spindle midzone • extracellular exosome • mitotic spindle • centralspindlin complex Biological process • mitotic cytokinesis • actomyosin contractile ring assembly • microtubule-based movement • small GTPase mediated signal transduction • spermatogenesis • neuroblast proliferation • blood coagulation • sulfate transport • embryo development • antigen processing and presentation of exogenous peptide antigen via MHC class II • positive regulation of cytokinesis • positive regulation of GTPase activity • regulation of small GTPase mediated signal transduction • mitotic spindle midzone assembly • regulation of attachment of spindle microtubules to kinetochore • mitotic spindle assembly Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	29127	26934
Ensembl	ENSG00000161800	ENSMUSG00000023015
UniProt	Q9H0H5	Q9WVM1
RefSeq (mRNA)	NM_001126103	NM_001253808
RefSeq (protein)	NP_001119575	NP_001240737
Location (UCSC)	Chr 12: 49.98 – 50.03 Mb	Chr 15: 99.62 – 99.65 Mb
PubMed search

Rac GTPase-activating protein 1 is an enzyme that in humans is encoded by the RACGAP1 gene.^[1]

Function

Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.[supplied by OMIM]^[2]

Interactions

RACGAP1 has been shown to interact with Rnd2^[3] and SLC26A8.^[4]

During cytokinesis, RACGAP1 has been shown to interact with KIF23 to form the centralspindlin complex.^[5]

References

1. Touré A, Dorseuil O, Morin L, Timmons P, Jégou B, Reibel L, Gacon G (April 1998). "MgcRacGAP, a new human GTPase-activating protein for Rac and Cdc42 similar to Drosophila rotundRacGAP gene product, is expressed in male germ cells". J Biol Chem 273 (11): 6019–23. doi:10.1074/jbc.273.11.6019. PMID 9497316.
2. "Entrez Gene: RACGAP1 Rac GTPase activating protein 1".
3. Naud N, Touré A, Liu J, Pineau C, Morin L, Dorseuil O, Escalier D, Chardin P, Gacon G (May 2003). "Rho family GTPase Rnd2 interacts and co-localizes with MgcRacGAP in male germ cells". Biochem. J. 372 (Pt 1): 105–12. doi:10.1042/BJ20021652. PMC 1223378. PMID 12590651.
4. Toure A, Morin L, Pineau C, Becq F, Dorseuil O, Gacon G (June 2001). "Tat1, a novel sulfate transporter specifically expressed in human male germ cells and potentially linked to rhogtpase signaling". J. Biol. Chem. 276 (23): 20309–15. doi:10.1074/jbc.M011740200. PMID 11278976.
5. Glotzer, Michael. "Cytokinesis: Centralspindlin Moonlights as a Membrane Anchor", Current Biology, 18 February 2013

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Gerwins P, Blank JL, Johnson GL (1997). "Cloning of a novel mitogen-activated protein kinase kinase kinase, MEKK4, that selectively regulates the c-Jun amino terminal kinase pathway.". J. Biol. Chem. 272 (13): 8288–95. doi:10.1074/jbc.272.13.8288. PMID 9079650.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Nagata Ki, Puls A, Futter C, Aspenstrom P, Schaefer E, Nakata T, Hirokawa N, Hall A (1998). "The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3.". EMBO J. 17 (1): 149–58. doi:10.1093/emboj/17.1.149. PMC 1170366. PMID 9427749.
• Tapon N, Nagata K, Lamarche N, Hall A (1998). "A new rac target POSH is an SH3-containing scaffold protein involved in the JNK and NF-kappaB signalling pathways.". EMBO J. 17 (5): 1395–404. doi:10.1093/emboj/17.5.1395. PMC 1170487. PMID 9482736.
• Abo A, Qu J, Cammarano MS, Dan C, Fritsch A, Baud V, Belisle B, Minden A (1999). "PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia.". EMBO J. 17 (22): 6527–40. doi:10.1093/emboj/17.22.6527. PMC 1171000. PMID 9822598.
• Zuber J, Tchernitsa OI, Hinzmann B, Schmitz AC, Grips M, Hellriegel M, Sers C, Rosenthal A, Schäfer R (2000). "A genome-wide survey of RAS transformation targets.". Nat. Genet. 24 (2): 144–52. doi:10.1038/72799. PMID 10655059.
• Das B, Shu X, Day GJ, Han J, Krishna UM, Falck JR, Broek D (2000). "Control of intramolecular interactions between the pleckstrin homology and Dbl homology domains of Vav and Sos1 regulates Rac binding.". J. Biol. Chem. 275 (20): 15074–81. doi:10.1074/jbc.M907269199. PMID 10748082.
• Nagase T, Kikuno R, Ishikawa K, Hirosawa M, Ohara O (2000). "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.". DNA Res. 7 (2): 143–50. doi:10.1093/dnares/7.2.143. PMID 10819331.
• Kawashima T, Hirose K, Satoh T, Kaneko A, Ikeda Y, Kaziro Y, Nosaka T, Kitamura T (2000). "MgcRacGAP is involved in the control of growth and differentiation of hematopoietic cells.". Blood 96 (6): 2116–24. PMID 10979956.
• Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination.". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Hirose K, Kawashima T, Iwamoto I, Nosaka T, Kitamura T (2001). "MgcRacGAP is involved in cytokinesis through associating with mitotic spindle and midbody.". J. Biol. Chem. 276 (8): 5821–8. doi:10.1074/jbc.M007252200. PMID 11085985.
• Caloca MJ, Wang H, Delemos A, Wang S, Kazanietz MG (2001). "Phorbol esters and related analogs regulate the subcellular localization of beta 2-chimaerin, a non-protein kinase C phorbol ester receptor.". J. Biol. Chem. 276 (21): 18303–12. doi:10.1074/jbc.M011368200. PMID 11278894.
• Toure A, Morin L, Pineau C, Becq F, Dorseuil O, Gacon G (2001). "Tat1, a novel sulfate transporter specifically expressed in human male germ cells and potentially linked to rhogtpase signaling.". J. Biol. Chem. 276 (23): 20309–15. doi:10.1074/jbc.M011740200. PMID 11278976.
• Mishima M, Kaitna S, Glotzer M (2002). "Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity.". Dev. Cell 2 (1): 41–54. doi:10.1016/S1534-5807(01)00110-1. PMID 11782313.
• Vikis HG, Li W, Guan KL (2002). "The plexin-B1/Rac interaction inhibits PAK activation and enhances Sema4D ligand binding.". Genes Dev. 16 (7): 836–45. doi:10.1101/gad.966402. PMC 186329. PMID 11937491.
• Kitamura T, Kawashima T, Minoshima Y, Tonozuka Y, Hirose K, Nosaka T (2002). "Role of MgcRacGAP/Cyk4 as a regulator of the small GTPase Rho family in cytokinesis and cell differentiation.". Cell Struct. Funct. 26 (6): 645–51. doi:10.1247/csf.26.645. PMID 11942621.
• Côté JF, Vuori K (2003). "Identification of an evolutionarily conserved superfamily of DOCK180-related proteins with guanine nucleotide exchange activity.". J. Cell. Sci. 115 (Pt 24): 4901–13. doi:10.1242/jcs.00219. PMID 12432077.
• Jantsch-Plunger V, Gönczy P, Romano A, Schnabel H, Hamill D, Schnabel R, Hyman AA, Glotzer M (2000). "CYK-4: A Rho family gtpase activating protein (GAP) required for central spindle formation and cytokinesis.". J Cell Biol 149 (7): 1391–404. doi:10.1083/jcb.149.7.1391. PMC 2175131. PMID 10871280.