Receptor for activated C kinase 1

Receptor for activated C kinase 1
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 4AOW​, 4V6X​, 5AJ0​
Identifiers
Symbols	RACK1 ; GNB2L1; Gnb2-rs1; H12.3; HLC-7; PIG21
External IDs	OMIM: 176981 MGI: 101849 HomoloGene: 4446 GeneCards: RACK1 Gene
Gene ontology Molecular function • protein kinase C binding • receptor binding • protein binding • ion channel inhibitor activity • cysteine-type endopeptidase activator activity involved in apoptotic process • enzyme binding • protein phosphatase binding • protein tyrosine kinase inhibitor activity • receptor tyrosine kinase binding • protein complex scaffold • signaling adaptor activity • SH2 domain binding • protein homodimerization activity • poly(A) RNA binding Cellular component • phagocytic cup • nucleus • cytoplasm • mitochondrion • cytosol • cytoskeleton • small ribosomal subunit • dendrite • midbody • neuronal cell body • perikaryon • perinuclear region of cytoplasm • extracellular exosome • IRE1-RACK1-PP2A complex Biological process • osteoblast differentiation • positive regulation of protein phosphorylation • activation of cysteine-type endopeptidase activity involved in apoptotic process • cell cycle • gastrulation • negative regulation of gene expression • viral process • negative regulation of translation • negative regulation of Wnt signaling pathway • negative regulation of cell growth • positive regulation of cell migration • positive regulation of cAMP catabolic process • positive regulation of proteasomal ubiquitin-dependent protein catabolic process • positive regulation of protein homooligomerization • regulation of protein localization • negative regulation of peptidyl-serine phosphorylation • positive regulation of apoptotic process • positive regulation of GTPase activity • rhythmic process • negative regulation of phagocytosis • regulation of cell division • positive regulation of cyclic-nucleotide phosphodiesterase activity • regulation of cell cycle • negative regulation of protein kinase B signaling • positive regulation of mitochondrial depolarization • negative regulation of protein tyrosine kinase activity • cellular response to glucose stimulus • regulation of establishment of protein localization to plasma membrane • negative regulation of endoplasmic reticulum unfolded protein response • negative regulation of hydrogen peroxide-induced neuron death • regulation of establishment of cell polarity • positive regulation of gastrulation • positive regulation of intrinsic apoptotic signaling pathway Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	10399	14694
Ensembl	ENSG00000204628	ENSMUSG00000020372
UniProt	P63244	P68040
RefSeq (mRNA)	NM_006098	NM_008143
RefSeq (protein)	NP_006089	NP_032169
Location (UCSC)	Chr 5: 181.24 – 181.25 Mb	Chr 11: 48.8 – 48.81 Mb
PubMed search

Receptor for activated C kinase 1 (RACK1, also known as guanine nucleotide-binding protein subunit beta-2-like 1 (GNB2L1), is a 32 kDa protein that in humans is encoded by the RACK1 gene.^[1][2]

Interactions

RACK1 has been shown to interact with::

• AGTRAP^[3]
• Androgen receptor,^[4]
• CD18,^[5]
• CD29^[5][6]
• Cyclin A1^[7]
• EIF6,^[8]
• FYN,^[9][10]
• IFNAR2,^[11][12]
• Janus kinase 1^[13]
• OTUB1,^[14]
• P73,^[15]
• PDE4D,^[16][17]
• PRKCB1,^[18]
• PRKCE,^[19]
• PTPRM,^[20][21]
• RAS p21 protein activator 1,^[22]
• ST7,^[23]
• STAT1,^[11][13]
• Src,^[24] and
• Tyrosine kinase 2.^[13]

See also

• RACK protein
• G protein

References

1. Ron D, Chen CH, Caldwell J, Jamieson L, Orr E, Mochly-Rosen D (Feb 1994). "Cloning of an intracellular receptor for protein kinase C: a homolog of the beta subunit of G proteins". Proceedings of the National Academy of Sciences of the United States of America 91 (3): 839–43. doi:10.1073/pnas.91.3.839. PMC 521407. PMID 8302854.
2. Guillemot F, Billault A, Auffray C (Jun 1989). "Physical linkage of a guanine nucleotide-binding protein-related gene to the chicken major histocompatibility complex". Proceedings of the National Academy of Sciences of the United States of America 86 (12): 4594–8. doi:10.1073/pnas.86.12.4594. PMC 287317. PMID 2499885.
3. Wang W, Huang Y, Zhou Z, Tang R, Zhao W, Zeng L, Xu M, Cheng C, Gu S, Ying K, Xie Y, Mao Y (Jan 2002). "Identification and characterization of AGTRAP, a human homolog of murine Angiotensin II Receptor-Associated Protein (Agtrap)". The International Journal of Biochemistry & Cell Biology 34 (1): 93–102. doi:10.1016/s1357-2725(01)00094-2. PMID 11733189.
4. Rigas AC, Ozanne DM, Neal DE, Robson CN (Nov 2003). "The scaffolding protein RACK1 interacts with androgen receptor and promotes cross-talk through a protein kinase C signaling pathway". The Journal of Biological Chemistry 278 (46): 46087–93. doi:10.1074/jbc.M306219200. PMID 12958311.
5. Liliental J, Chang DD (Jan 1998). "Rack1, a receptor for activated protein kinase C, interacts with integrin beta subunit". The Journal of Biological Chemistry 273 (4): 2379–83. doi:10.1074/jbc.273.4.2379. PMID 9442085.
6. Lee HS, Millward-Sadler SJ, Wright MO, Nuki G, Al-Jamal R, Salter DM (Nov 2002). "Activation of Integrin-RACK1/PKCalpha signalling in human articular chondrocyte mechanotransduction". Osteoarthritis and Cartilage / OARS, Osteoarthritis Research Society 10 (11): 890–7. doi:10.1053/joca.2002.0842. PMID 12435334.
7. Diederichs S, Bäumer N, Ji P, Metzelder SK, Idos GE, Cauvet T, Wang W, Möller M, Pierschalski S, Gromoll J, Schrader MG, Koeffler HP, Berdel WE, Serve H, Müller-Tidow C (Aug 2004). "Identification of interaction partners and substrates of the cyclin A1-CDK2 complex". The Journal of Biological Chemistry 279 (32): 33727–41. doi:10.1074/jbc.M401708200. PMID 15159402.
8. Ceci M, Gaviraghi C, Gorrini C, Sala LA, Offenhäuser N, Marchisio PC, Biffo S (Dec 2003). "Release of eIF6 (p27BBP) from the 60S subunit allows 80S ribosome assembly". Nature 426 (6966): 579–84. doi:10.1038/nature02160. PMID 14654845.
9. Yaka R, He DY, Phamluong K, Ron D (Mar 2003). "Pituitary adenylate cyclase-activating polypeptide (PACAP(1-38)) enhances N-methyl-D-aspartate receptor function and brain-derived neurotrophic factor expression via RACK1". The Journal of Biological Chemistry 278 (11): 9630–8. doi:10.1074/jbc.M209141200. PMID 12524444.
10. Yaka R, Thornton C, Vagts AJ, Phamluong K, Bonci A, Ron D (Apr 2002). "NMDA receptor function is regulated by the inhibitory scaffolding protein, RACK1". Proceedings of the National Academy of Sciences of the United States of America 99 (8): 5710–5. doi:10.1073/pnas.062046299. PMC 122836. PMID 11943848.
11. Usacheva A, Smith R, Minshall R, Baida G, Seng S, Croze E, Colamonici O (Jun 2001). "The WD motif-containing protein receptor for activated protein kinase C (RACK1) is required for recruitment and activation of signal transducer and activator of transcription 1 through the type I interferon receptor". The Journal of Biological Chemistry 276 (25): 22948–53. doi:10.1074/jbc.M100087200. PMID 11301323.
12. Croze E, Usacheva A, Asarnow D, Minshall RD, Perez HD, Colamonici O (Nov 2000). "Receptor for activated C-kinase (RACK-1), a WD motif-containing protein, specifically associates with the human type I IFN receptor". Journal of Immunology 165 (9): 5127–32. doi:10.4049/jimmunol.165.9.5127. PMID 11046044.
13. Usacheva A, Tian X, Sandoval R, Salvi D, Levy D, Colamonici OR (Sep 2003). "The WD motif-containing protein RACK-1 functions as a scaffold protein within the type I IFN receptor-signaling complex". Journal of Immunology 171 (6): 2989–94. doi:10.4049/jimmunol.171.6.2989. PMID 12960323.
14. Edelmann MJ, Iphöfer A, Akutsu M, Altun M, di Gleria K, Kramer HB, Fiebiger E, Dhe-Paganon S, Kessler BM (Mar 2009). "Structural basis and specificity of human otubain 1-mediated deubiquitination". The Biochemical Journal 418 (2): 379–90. doi:10.1042/BJ20081318. PMID 18954305.
15. Ozaki T, Watanabe K, Nakagawa T, Miyazaki K, Takahashi M, Nakagawara A (May 2003). "Function of p73, not of p53, is inhibited by the physical interaction with RACK1 and its inhibitory effect is counteracted by pRB". Oncogene 22 (21): 3231–42. doi:10.1038/sj.onc.1206382. PMID 12761493.
16. Yarwood SJ, Steele MR, Scotland G, Houslay MD, Bolger GB (May 1999). "The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform". The Journal of Biological Chemistry 274 (21): 14909–17. doi:10.1074/jbc.274.21.14909. PMID 10329691.
17. Steele MR, McCahill A, Thompson DS, MacKenzie C, Isaacs NW, Houslay MD, Bolger GB (Jul 2001). "Identification of a surface on the beta-propeller protein RACK1 that interacts with the cAMP-specific phosphodiesterase PDE4D5". Cellular Signalling 13 (7): 507–13. doi:10.1016/s0898-6568(01)00167-x. PMID 11516626.
18. Ron D, Jiang Z, Yao L, Vagts A, Diamond I, Gordon A (Sep 1999). "Coordinated movement of RACK1 with activated betaIIPKC". The Journal of Biological Chemistry 274 (38): 27039–46. doi:10.1074/jbc.274.38.27039. PMID 10480917.
19. Liedtke CM, Yun CH, Kyle N, Wang D (Jun 2002). "Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor". The Journal of Biological Chemistry 277 (25): 22925–33. doi:10.1074/jbc.M201917200. PMID 11956211.
20. Hellberg CB, Burden-Gulley SM, Pietz GE, Brady-Kalnay SM (Mar 2002). "Expression of the receptor protein-tyrosine phosphatase, PTPmu, restores E-cadherin-dependent adhesion in human prostate carcinoma cells". The Journal of Biological Chemistry 277 (13): 11165–73. doi:10.1074/jbc.M112157200. PMID 11801604.
21. Mourton T, Hellberg CB, Burden-Gulley SM, Hinman J, Rhee A, Brady-Kalnay SM (May 2001). "The PTPmu protein-tyrosine phosphatase binds and recruits the scaffolding protein RACK1 to cell-cell contacts". The Journal of Biological Chemistry 276 (18): 14896–901. doi:10.1074/jbc.M010823200. PMID 11278757.
22. Koehler JA, Moran MF (May 2001). "RACK1, a protein kinase C scaffolding protein, interacts with the PH domain of p120GAP". Biochemical and Biophysical Research Communications 283 (4): 888–95. doi:10.1006/bbrc.2001.4889. PMID 11350068.
23. Battle MA, Maher VM, McCormick JJ (Jun 2003). "ST7 is a novel low-density lipoprotein receptor-related protein (LRP) with a cytoplasmic tail that interacts with proteins related to signal transduction pathways". Biochemistry 42 (24): 7270–82. doi:10.1021/bi034081y. PMID 12809483.
24. Chang BY, Conroy KB, Machleder EM, Cartwright CA (Jun 1998). "RACK1, a receptor for activated C kinase and a homolog of the beta subunit of G proteins, inhibits activity of src tyrosine kinases and growth of NIH 3T3 cells". Molecular and Cellular Biology 18 (6): 3245–56. doi:10.1128/mcb.18.6.3245. PMC 108906. PMID 9584165.

Further reading

• Liu YV, Semenza GL (Mar 2007). "RACK1 vs. HSP90: competition for HIF-1 alpha degradation vs. stabilization". Cell Cycle 6 (6): 656–9. doi:10.4161/cc.6.6.3981. PMID 17361105.
• Mochly-Rosen D, Miller KG, Scheller RH, Khaner H, Lopez J, Smith BL (Sep 1992). "p65 fragments, homologous to the C2 region of protein kinase C, bind to the intracellular receptors for protein kinase C". Biochemistry 31 (35): 8120–4. doi:10.1021/bi00150a003. PMID 1326322.
• Dawson SJ, White LA (May 1992). "Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin". The Journal of Infection 24 (3): 317–20. doi:10.1016/S0163-4453(05)80037-4. PMID 1602151.
• Pumiglia KM, LeVine H, Haske T, Habib T, Jove R, Decker SJ (Jun 1995). "A direct interaction between G-protein beta gamma subunits and the Raf-1 protein kinase". The Journal of Biological Chemistry 270 (24): 14251–4. doi:10.1074/jbc.270.24.14251. PMID 7782277.
• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Disatnik MH, Hernandez-Sotomayor SM, Jones G, Carpenter G, Mochly-Rosen D (Jan 1994). "Phospholipase C-gamma 1 binding to intracellular receptors for activated protein kinase C". Proceedings of the National Academy of Sciences of the United States of America 91 (2): 559–63. doi:10.1073/pnas.91.2.559. PMC 42988. PMID 8290562.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Liliental J, Chang DD (Jan 1998). "Rack1, a receptor for activated protein kinase C, interacts with integrin beta subunit". The Journal of Biological Chemistry 273 (4): 2379–83. doi:10.1074/jbc.273.4.2379. PMID 9442085.
• Chang BY, Conroy KB, Machleder EM, Cartwright CA (Jun 1998). "RACK1, a receptor for activated C kinase and a homolog of the beta subunit of G proteins, inhibits activity of src tyrosine kinases and growth of NIH 3T3 cells". Molecular and Cellular Biology 18 (6): 3245–56. doi:10.1128/mcb.18.6.3245. PMC 108906. PMID 9584165.
• Rietzler M, Bittner M, Kolanus W, Schuster A, Holzmann B (Oct 1998). "The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins". The Journal of Biological Chemistry 273 (42): 27459–66. doi:10.1074/jbc.273.42.27459. PMID 9765275.
• Shimohama S, Kamiya S, Taniguchi T, Kimura J (Dec 1998). "Intracellular receptors for activated C-kinase in the postmortem human brain: no alteration in Alzheimer disease". Alzheimer Disease and Associated Disorders 12 (4): 384–6. doi:10.1097/00002093-199812000-00022. PMID 9876970.
• Yarwood SJ, Steele MR, Scotland G, Houslay MD, Bolger GB (May 1999). "The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform". The Journal of Biological Chemistry 274 (21): 14909–17. doi:10.1074/jbc.274.21.14909. PMID 10329691.
• Ron D, Jiang Z, Yao L, Vagts A, Diamond I, Gordon A (Sep 1999). "Coordinated movement of RACK1 with activated betaIIPKC". The Journal of Biological Chemistry 274 (38): 27039–46. doi:10.1074/jbc.274.38.27039. PMID 10480917.
• Geijsen N, Spaargaren M, Raaijmakers JA, Lammers JW, Koenderman L, Coffer PJ (Sep 1999). "Association of RACK1 and PKCbeta with the common beta-chain of the IL-5/IL-3/GM-CSF receptor". Oncogene 18 (36): 5126–30. doi:10.1038/sj.onc.1202896. PMID 10490850.
• Smith PR, de Jesus O, Turner D, Hollyoake M, Karstegl CE, Griffin BE, Karran L, Wang Y, Hayward SD, Farrell PJ (Apr 2000). "Structure and coding content of CST (BART) family RNAs of Epstein-Barr virus". Journal of Virology 74 (7): 3082–92. doi:10.1128/JVI.74.7.3082-3092.2000. PMC 111807. PMID 10708423.
• Ellis JH, Ashman C, Burden MN, Kilpatrick KE, Morse MA, Hamblin PA (Jun 2000). "GRID: a novel Grb-2-related adapter protein that interacts with the activated T cell costimulatory receptor CD28". Journal of Immunology 164 (11): 5805–14. doi:10.4049/jimmunol.164.11.5805. PMID 10820259.
• Croze E, Usacheva A, Asarnow D, Minshall RD, Perez HD, Colamonici O (Nov 2000). "Receptor for activated C-kinase (RACK-1), a WD motif-containing protein, specifically associates with the human type I IFN receptor". Journal of Immunology 165 (9): 5127–32. doi:10.4049/jimmunol.165.9.5127. PMID 11046044.
• Hartley JL, Temple GF, Brasch MA (Nov 2000). "DNA cloning using in vitro site-specific recombination". Genome Research 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.