EIF6

Eukaryotic translation initiation factor 6
Identifiers
Symbols	EIF6 ; CAB; EIF3A; ITGB4BP; b(2)gcn; eIF-6; p27(BBP); p27BBP
External IDs	OMIM: 602912 MGI: 1196288 HomoloGene: 7135 GeneCards: EIF6 Gene
EC number	3.6.5.3
Gene ontology Molecular function • translation initiation factor activity • protein binding • ribosome binding • ribosomal large subunit binding Cellular component • nucleus • lamin filament • nucleoplasm • nucleolus • cytoplasm • extracellular exosome Biological process • ribosomal subunit export from nucleus • translational initiation • mature ribosome assembly • ribosomal large subunit biogenesis Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	3692	16418
Ensembl	ENSG00000242372	ENSMUSG00000027613
UniProt	P56537	O55135
RefSeq (mRNA)	NM_001267810	NM_010579
RefSeq (protein)	NP_001254739	NP_034709
Location (UCSC)	Chr 20: 35.28 – 35.28 Mb	Chr 2: 155.82 – 155.83 Mb
PubMed search

Eukaryotic translation initiation factor 6 (EIF6), also known as Integrin beta 4 binding protein (ITGB4BP), is a human gene.^[1]

Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and catalyzes the association of the 40S and 60S ribosomal subunits along with eIF5 bound to GTP. Multiple transcript variants encoding several different isoforms have been found for this gene.^[1]

Interactions

EIF6 has been shown to interact with FHL2,^[2] ITGB4^[3] and GNB2L1.^[4]

References

1. "Entrez Gene: ITGB4BP integrin beta 4 binding protein".
2. Wixler, V; Geerts D; Laplantine E; Westhoff D; Smyth N; Aumailley M; Sonnenberg A; Paulsson M (October 2000). "The LIM-only protein DRAL/FHL2 binds to the cytoplasmic domain of several alpha and beta integrin chains and is recruited to adhesion complexes". J. Biol. Chem. (UNITED STATES) 275 (43): 33669–78. doi:10.1074/jbc.M002519200. ISSN 0021-9258. PMID 10906324.
3. Biffo, S; Sanvito F; Costa S; Preve L; Pignatelli R; Spinardi L; Marchisio P C (November 1997). "Isolation of a novel beta4 integrin-binding protein (p27(BBP)) highly expressed in epithelial cells". J. Biol. Chem. (UNITED STATES) 272 (48): 30314–21. doi:10.1074/jbc.272.48.30314. ISSN 0021-9258. PMID 9374518.
4. Ceci, Marcello; Gaviraghi Cristina; Gorrini Chiara; Sala Leonardo A; Offenhäuser Nina; Marchisio Pier Carlo; Biffo Stefano (December 2003). "Release of eIF6 (p27BBP) from the 60S subunit allows 80S ribosome assembly". Nature (England) 426 (6966): 579–84. doi:10.1038/nature02160. PMID 14654845.

Further reading

• Biffo S, Sanvito F, Costa S; et al. (1997). "Isolation of a novel beta4 integrin-binding protein (p27(BBP)) highly expressed in epithelial cells". J. Biol. Chem. 272 (48): 30314–21. doi:10.1074/jbc.272.48.30314. PMID 9374518.
• Si K, Chaudhuri J, Chevesich J, Maitra U (1998). "Molecular cloning and functional expression of a human cDNA encoding translation initiation factor 6". Proc. Natl. Acad. Sci. U.S.A. 94 (26): 14285–90. doi:10.1073/pnas.94.26.14285. PMC 24943. PMID 9405604.
• Mao M, Fu G, Wu JS; et al. (1998). "Identification of genes expressed in human CD34+ hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning". Proc. Natl. Acad. Sci. U.S.A. 95 (14): 8175–80. doi:10.1073/pnas.95.14.8175. PMC 20949. PMID 9653160.
• Sanvito F, Arrigo G, Zuffardi O; et al. (1998). "Localization of p27 beta4 binding protein gene (ITGB4BP) to human chromosome region 20q11.2". Genomics 52 (1): 111–2. doi:10.1006/geno.1998.5403. PMID 9740680.
• Sanvito F, Piatti S, Villa A; et al. (1999). "The β4 Integrin Interactor p27BBP/eIF6 Is an Essential Nuclear Matrix Protein Involved in 60S Ribosomal Subunit Assembly". J. Cell Biol. 144 (5): 823–37. doi:10.1083/jcb.144.5.823. PMC 2148184. PMID 10085284.
• Wixler V, Geerts D, Laplantine E; et al. (2000). "The LIM-only protein DRAL/FHL2 binds to the cytoplasmic domain of several alpha and beta integrin chains and is recruited to adhesion complexes". J. Biol. Chem. 275 (43): 33669–78. doi:10.1074/jbc.M002519200. PMID 10906324.
• Zhang QH, Ye M, Wu XY; et al. (2001). "Cloning and Functional Analysis of cDNAs with Open Reading Frames for 300 Previously Undefined Genes Expressed in CD34+ Hematopoietic Stem/Progenitor Cells". Genome Res. 10 (10): 1546–60. doi:10.1101/gr.140200. PMC 310934. PMID 11042152.
• Donadini A, Giodini A, Sanvito F; et al. (2001). "The human ITGB4BP gene is constitutively expressed in vitro, but highly modulated in vivo". Gene 266 (1–2): 35–43. doi:10.1016/S0378-1119(01)00370-5. PMID 11290417.
• Deloukas P, Matthews LH, Ashurst J; et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20". Nature 414 (6866): 865–71. doi:10.1038/414865a. PMID 11780052.
• Andersen JS, Lyon CE, Fox AH; et al. (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Basu U, Si K, Deng H, Maitra U (2003). "Phosphorylation of Mammalian Eukaryotic Translation Initiation Factor 6 and Its Saccharomyces cerevisiae Homologue Tif6p: Evidence that Phosphorylation of Tif6p Regulates Its Nucleocytoplasmic Distribution and Is Required for Yeast Cell Growth". Mol. Cell. Biol. 23 (17): 6187–99. doi:10.1128/MCB.23.17.6187-6199.2003. PMC 180954. PMID 12917340.
• Ceci M, Gaviraghi C, Gorrini C; et al. (2003). "Release of eIF6 (p27BBP) from the 60S subunit allows 80S ribosome assembly". Nature 426 (6966): 579–84. doi:10.1038/nature02160. PMID 14654845.
• Rosso P, Cortesina G, Sanvito F; et al. (2004). "Overexpression of p27BBP in head and neck carcinomas and their lymph node metastases". Head Neck 26 (5): 408–17. doi:10.1002/hed.10401. PMID 15122657.
• Lehner B, Sanderson CM (2004). "A Protein Interaction Framework for Human mRNA Degradation". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Rush J, Moritz A, Lee KA; et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455.
• Andersen JS, Lam YW, Leung AK; et al. (2005). "Nucleolar proteome dynamics". Nature 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413.
• Stelzl U, Worm U, Lalowski M; et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.