RASSF1

Ras association (RalGDS/AF-6) domain family member 1
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2KZU​
Identifiers
Symbols	RASSF1 ; 123F2; NORE2A; RASSF1A; RDA32; REH3P21
External IDs	OMIM: 605082 HomoloGene: 10499 GeneCards: RASSF1 Gene
Gene ontology Molecular function • protein binding • zinc ion binding • identical protein binding • protein N-terminus binding Cellular component • spindle pole • nucleus • cytoplasm • microtubule organizing center • microtubule • plasma membrane • microtubule cytoskeleton Biological process • cellular response to DNA damage stimulus • cell cycle arrest • Ras protein signal transduction • positive regulation of protein ubiquitination • protein stabilization • negative regulation of cell cycle arrest Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	11186	56289
Ensembl	ENSG00000068028	ENSMUSG00000010067
UniProt	Q9NS23	Q99MK9
RefSeq (mRNA)	NM_001206957	NM_001243748
RefSeq (protein)	NP_001193886	NP_001230677
Location (UCSC)	Chr 3: 50.33 – 50.34 Mb	Chr 9: 107.55 – 107.56 Mb
PubMed search

Ras association domain-containing protein 1 is a protein that in humans is encoded by the RASSF1 gene.

Function

This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.^[1]

Interactions

RASSF1 has been shown to interact with:

• CNKSR1,^[2]
• Death associated protein 6^[3]
• HRAS,^[4]
• MAP1B,^[5]
• MAP1S,^[5][6] and
• RASSF5.^[7]

References

1. "Entrez Gene: RASSF1 Ras association (RalGDS/AF-6) domain family 1".
2. Rabizadeh S, Xavier RJ, Ishiguro K, Bernabeortiz J, Lopez-Ilasaca M, Khokhlatchev A, Mollahan P, Pfeifer GP, Avruch J, Seed B (Jul 2004). "The scaffold protein CNK1 interacts with the tumor suppressor RASSF1A and augments RASSF1A-induced cell death". The Journal of Biological Chemistry 279 (28): 29247–54. doi:10.1074/jbc.M401699200. PMID 15075335.
3. Song MS, Song SJ, Kim SY, Oh HJ, Lim DS (Jul 2008). "The tumour suppressor RASSF1A promotes MDM2 self-ubiquitination by disrupting the MDM2-DAXX-HAUSP complex". The EMBO Journal 27 (13): 1863–74. doi:10.1038/emboj.2008.115. PMC 2486425. PMID 18566590.
4. Vos MD, Ellis CA, Bell A, Birrer MJ, Clark GJ (Nov 2000). "Ras uses the novel tumor suppressor RASSF1 as an effector to mediate apoptosis". The Journal of Biological Chemistry 275 (46): 35669–72. doi:10.1074/jbc.C000463200. PMID 10998413.
5. Dallol A, Agathanggelou A, Fenton SL, Ahmed-Choudhury J, Hesson L, Vos MD, Clark GJ, Downward J, Maher ER, Latif F (Jun 2004). "RASSF1A interacts with microtubule-associated proteins and modulates microtubule dynamics". Cancer Research 64 (12): 4112–6. doi:10.1158/0008-5472.CAN-04-0267. PMID 15205320.
6. Liu L, Vo A, McKeehan WL (Mar 2005). "Specificity of the methylation-suppressed A isoform of candidate tumor suppressor RASSF1 for microtubule hyperstabilization is determined by cell death inducer C19ORF5". Cancer Research 65 (5): 1830–8. doi:10.1158/0008-5472.CAN-04-3896. PMID 15753381.
7. Ortiz-Vega S, Khokhlatchev A, Nedwidek M, Zhang XF, Dammann R, Pfeifer GP, Avruch J (Feb 2002). "The putative tumor suppressor RASSF1A homodimerizes and heterodimerizes with the Ras-GTP binding protein Nore1". Oncogene 21 (9): 1381–90. doi:10.1038/sj.onc.1205192. PMID 11857081.