RNAI

For RNA interference, see RNAi.
RNAI
Predicted secondary structure and sequence conservation of RNAI
Identifiers
Symbol RNAI
Rfam RF00106
Other data
RNA type Gene; antisense
Domain(s) Bacteria
SO 0000644

RNAI is a non-coding RNA that is an antisense repressor of the replication of some E. coli plasmids, including ColE1. Plasmid replication is usually initiated by RNAII,[1] which acts as a primer by binding to its template DNA. The complementary RNAI binds RNAII prohibiting it from its initiation role. The rate of degradation of RNAI is therefore a major factor in control of plasmid replication. This rate of degradation is aided by the pcnB (plasmid copy number B) gene product,[2] which polyadenylates the 3' end of RNAI targeting it for degradation by PNPase.[3]

References

  1. Masukata, Hisao; Tomizawa, J-I (1986). "Control of primer formation for ColE1 plasmid replication: conformational change of the primer transcript". Cell 44: 125136. doi:10.1016/0092-8674(86)90491-5. PMID 2416472.
  2. He, L; Soderbom F; Wagner EG; Binnie U; Binns N; Masters M (1993). "PcnB is required for the rapid degradation of RNAI, the antisense RNA that controls the copy number of ColE1-related plasmids". Mol Microbiol 9 (6): 11311142. doi:10.1111/j.1365-2958.1993.tb01243.x. PMID 7523833.
  3. Xu, F; Lin-Chao S; Cohen SN (1993). "The Escherichia coli pcnB gene promotes adenylylation of antisense RNAI of ColE1-type plasmids in vivo and degradation of RNAI decay intermediates". Proc Natl Acad Sci USA 90 (14): 67566760. doi:10.1073/pnas.90.14.6756. PMC 47011. PMID 7688127.

External links


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