STXBP1

Syntaxin binding protein 1
PDB rendering based on 1dn1.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1dn1​
Identifiers
Symbols	STXBP1 ; MUNC18-1; NSEC1; P67; RBSEC1; UNC18
External IDs	OMIM: 602926 MGI: 107363 HomoloGene: 2382 GeneCards: STXBP1 Gene
Gene ontology Molecular function • SNARE binding • protein binding • syntaxin-1 binding • protein kinase binding • protein domain specific binding • syntaxin binding • identical protein binding • poly(A) RNA binding • protein N-terminus binding Cellular component • nucleoplasm • cytoplasm • mitochondrion • cytosol • plasma membrane • platelet alpha granule • terminal bouton • myelin sheath • protein complex • extracellular exosome Biological process • platelet degranulation • energy reserve metabolic process • vesicle docking involved in exocytosis • synaptic transmission • neurotransmitter secretion • neuromuscular synaptic transmission • axon target recognition • regulation of synaptic vesicle priming • glutamate secretion • protein transport • synaptic vesicle maturation • regulation of synaptic vesicle fusion to presynaptic membrane • negative regulation of synaptic transmission, GABAergic • regulation of SNARE complex assembly • negative regulation of neuron apoptotic process • small molecule metabolic process • positive regulation of calcium ion-dependent exocytosis • regulation of insulin secretion • protein stabilization • long term synaptic depression • platelet aggregation • protein localization to plasma membrane Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	6812	20910
Ensembl	ENSG00000136854	ENSMUSG00000026797
UniProt	P61764	O08599
RefSeq (mRNA)	NM_001032221	NM_001113569
RefSeq (protein)	NP_001027392	NP_001107041
Location (UCSC)	Chr 9: 127.61 – 127.69 Mb	Chr 2: 32.79 – 32.85 Mb
PubMed search

Syntaxin-binding protein 1 is a protein that in humans is encoded by the STXBP1 gene.^[1][2]

In melanocytic cells STXBP1 gene expression may be regulated by MITF.^[3]

Interactions

STXBP1 has been shown to interact with STX2,^[4][5] STX4^[4][5] and STX1A.^{[5][6][7][8][9]}

References

1. Swanson DA, Steel JM, Valle D (Jun 1998). "Identification and characterization of the human ortholog of rat STXBP1, a protein implicated in vesicle trafficking and neurotransmitter release". Genomics 48 (3): 373–6. doi:10.1006/geno.1997.5202. PMID 9545644.
2. "Entrez Gene: STXBP1 syntaxin binding protein 1".
3. Hoek KS, Schlegel NC, Eichhoff OM, Widmer DS, Praetorius C, Einarsson SO, Valgeirsdottir S, Bergsteinsdottir K, Schepsky A, Dummer R, Steingrimsson E (2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell Melanoma Res 21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x. PMID 19067971.
4. Schraw TD, Lemons PP, Dean WL, Whiteheart SW (Aug 2003). "A role for Sec1/Munc18 proteins in platelet exocytosis". Biochem. J. 374 (Pt 1): 207–17. doi:10.1042/BJ20030610. PMC 1223584. PMID 12773094.
5. Hata Y, Südhof TC (Jun 1995). "A novel ubiquitous form of Munc-18 interacts with multiple syntaxins. Use of the yeast two-hybrid system to study interactions between proteins involved in membrane traffic". J. Biol. Chem. 270 (22): 13022–8. doi:10.1074/jbc.270.22.13022. PMID 7768895.
6. Bhaskar K, Shareef MM, Sharma VM, Shetty AP, Ramamohan Y, Pant HC, Raju TR, Shetty KT (Jan 2004). "Co-purification and localization of Munc18-1 (p67) and Cdk5 with neuronal cytoskeletal proteins". Neurochem. Int. 44 (1): 35–44. doi:10.1016/S0197-0186(03)00099-8. PMID 12963086.
7. Dulubova I, Sugita S, Hill S, Hosaka M, Fernandez I, Südhof TC, Rizo J (Aug 1999). "A conformational switch in syntaxin during exocytosis: role of munc18". EMBO J. 18 (16): 4372–82. doi:10.1093/emboj/18.16.4372. PMC 1171512. PMID 10449403.
8. McMahon HT, Missler M, Li C, Südhof TC (Oct 1995). "Complexins: cytosolic proteins that regulate SNAP receptor function". Cell 83 (1): 111–9. doi:10.1016/0092-8674(95)90239-2. PMID 7553862.
9. Pérez-Brangulí F, Muhaisen A, Blasi J (Jun 2002). "Munc 18a binding to syntaxin 1A and 1B isoforms defines its localization at the plasma membrane and blocks SNARE assembly in a three-hybrid system assay". Mol. Cell. Neurosci. 20 (2): 169–80. doi:10.1006/mcne.2002.1122. PMID 12093152.

Further reading

• Garcia EP, McPherson PS, Chilcote TJ, Takei K, De Camilli P (1995). "rbSec1A and B colocalize with syntaxin 1 and SNAP-25 throughout the axon, but are not in a stable complex with syntaxin". J. Cell Biol. 129 (1): 105–20. doi:10.1083/jcb.129.1.105. PMC 2120371. PMID 7698978.
• Pevsner J, Hsu SC, Scheller RH (1994). "n-Sec1: a neural-specific syntaxin-binding protein". Proc. Natl. Acad. Sci. U.S.A. 91 (4): 1445–9. doi:10.1073/pnas.91.4.1445. PMC 43176. PMID 8108429.
• Fujita Y, Sasaki T, Fukui K, Kotani H, Kimura T, Hata Y, Südhof TC, Scheller RH, Takai Y (1996). "Phosphorylation of Munc-18/n-Sec1/rbSec1 by protein kinase C: its implication in regulating the interaction of Munc-18/n-Sec1/rbSec1 with syntaxin". J. Biol. Chem. 271 (13): 7265–8. doi:10.1074/jbc.271.13.7265. PMID 8631738.
• Gengyo-Ando K, Kitayama H, Mukaida M, Ikawa Y (1996). "A murine neural-specific homolog corrects cholinergic defects in Caenorhabditis elegans unc-18 mutants". J. Neurosci. 16 (21): 6695–702. PMID 8824310.
• Tellam JT, Macaulay SL, McIntosh S, Hewish DR, Ward CW, James DE (1997). "Characterization of Munc-18c and syntaxin-4 in 3T3-L1 adipocytes. Putative role in insulin-dependent movement of GLUT-4". J. Biol. Chem. 272 (10): 6179–86. doi:10.1074/jbc.272.10.6179. PMID 9045631.
• Verhage M, de Vries KJ, Røshol H, Burbach JP, Gispen WH, Südhof TC (1997). "DOC2 proteins in rat brain: complementary distribution and proposed function as vesicular adapter proteins in early stages of secretion". Neuron 18 (3): 453–61. doi:10.1016/S0896-6273(00)81245-3. PMID 9115738.
• Okamoto M, Südhof TC (1997). "Mints, Munc18-interacting proteins in synaptic vesicle exocytosis". J. Biol. Chem. 272 (50): 31459–64. doi:10.1074/jbc.272.50.31459. PMID 9395480.
• Fletcher AI, Shuang R, Giovannucci DR, Zhang L, Bittner MA, Stuenkel EL (1999). "Regulation of exocytosis by cyclin-dependent kinase 5 via phosphorylation of Munc18". J. Biol. Chem. 274 (7): 4027–35. doi:10.1074/jbc.274.7.4027. PMID 9933594.
• Reed GL, Houng AK, Fitzgerald ML (1999). "Human platelets contain SNARE proteins and a Sec1p homologue that interacts with syntaxin 4 and is phosphorylated after thrombin activation: implications for platelet secretion". Blood 93 (8): 2617–26. PMID 10194441.
• Dulubova I, Sugita S, Hill S, Hosaka M, Fernandez I, Südhof TC, Rizo J (1999). "A conformational switch in syntaxin during exocytosis: role of munc18". EMBO J. 18 (16): 4372–82. doi:10.1093/emboj/18.16.4372. PMC 1171512. PMID 10449403.
• Misura KM, Scheller RH, Weis WI (2000). "Three-dimensional structure of the neuronal-Sec1-syntaxin 1a complex". Nature 404 (6776): 355–62. doi:10.1038/35006120. PMID 10746715.
• Allan BB, Moyer BD, Balch WE (2000). "Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion". Science 289 (5478): 444–8. doi:10.1126/science.289.5478.444. PMID 10903204.
• Shorter J, Beard MB, Seemann J, Dirac-Svejstrup AB, Warren G (2002). "Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115". J. Cell Biol. 157 (1): 45–62. doi:10.1083/jcb.200112127. PMC 2173270. PMID 11927603.
• Ho CS, Marinescu V, Steinhilb ML, Gaut JR, Turner RS, Stuenkel EL (2002). "Synergistic effects of Munc18a and X11 proteins on amyloid precursor protein metabolism". J. Biol. Chem. 277 (30): 27021–8. doi:10.1074/jbc.M201823200. PMID 12016213.
• Pérez-Brangulí F, Muhaisen A, Blasi J (2002). "Munc 18a binding to syntaxin 1A and 1B isoforms defines its localization at the plasma membrane and blocks SNARE assembly in a three-hybrid system assay". Mol. Cell. Neurosci. 20 (2): 169–80. doi:10.1006/mcne.2002.1122. PMID 12093152.
• Barclay JW, Craig TJ, Fisher RJ, Ciufo LF, Evans GJ, Morgan A, Burgoyne RD (2003). "Phosphorylation of Munc18 by protein kinase C regulates the kinetics of exocytosis". J. Biol. Chem. 278 (12): 10538–45. doi:10.1074/jbc.M211114200. PMID 12519779.
• Fukuda M (2003). "Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells". J. Biol. Chem. 278 (17): 15390–6. doi:10.1074/jbc.M213090200. PMID 12590134.
• Craig TJ, Evans GJ, Morgan A (2003). "Physiological regulation of Munc18/nSec1 phosphorylation on serine-313". J. Neurochem. 86 (6): 1450–7. doi:10.1046/j.1471-4159.2003.01955.x. PMID 12950453.