SUPT5H

Suppressor of Ty 5 homolog (S. cerevisiae)
PDB rendering based on 2do3.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2DO3​, 2E6Z​, 2E70​, 3H7H​, 4L1U​
Identifiers
Symbols	SUPT5H ; SPT5; SPT5H; Tat-CT1
External IDs	OMIM: 602102 MGI: 1202400 HomoloGene: 2384 GeneCards: SUPT5H Gene
Gene ontology Molecular function • chromatin binding • protein binding • enzyme binding • poly(A) RNA binding • protein heterodimerization activity Cellular component • nucleus • nucleoplasm • DSIF complex Biological process • negative regulation of transcription from RNA polymerase II promoter • chromatin remodeling • DNA-templated transcription, elongation • transcription from RNA polymerase II promoter • transcription elongation from RNA polymerase II promoter • 7-methylguanosine mRNA capping • cell cycle • response to organic substance • gene expression • viral process • positive regulation of macroautophagy • negative regulation of DNA-templated transcription, elongation • positive regulation of DNA-templated transcription, elongation • single stranded viral RNA replication via double stranded DNA intermediate • positive regulation of transcription from RNA polymerase II promoter • positive regulation of viral transcription • negative regulation of mRNA polyadenylation Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	6829	20924
Ensembl	ENSG00000196235	ENSMUSG00000003435
UniProt	O00267	O55201
RefSeq (mRNA)	NM_001111020	NM_013676
RefSeq (protein)	NP_001104490	NP_038704
Location (UCSC)	Chr 19: 39.44 – 39.48 Mb	Chr 7: 28.31 – 28.34 Mb
PubMed search

Transcription elongation factor SPT5 is a protein that in humans is encoded by the SUPT5H gene.^[1][2]

Interactions

SUPT5H has been shown to interact with:

• CDK9,^[3]
• Cyclin-dependent kinase 7,^[3]
• HTATSF1,^[4]
• PIN1,^[5]
• POLR2A,^[4][6]
• PRMT1^[7] and
• Protein arginine methyltransferase 5.^[7]

Model organisms

Model organisms have been used in the study of SUPT5H function. A conditional knockout mouse line called Supt5^{tm2a(KOMP)Wtsi} was generated at the Wellcome Trust Sanger Institute.^[8] Male and female animals underwent a standardized phenotypic screen^[9] to determine the effects of deletion.^{[10][11][12][13]} Additional screens performed: - In-depth immunological phenotyping^[14]

Supt5 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[9][14]
Peripheral blood leukocytes 6 Weeks	Normal
Haematology 6 Weeks	Normal
Homozygous viability at P14	Abnormal
Recessive lethal study	Abnormal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Cytotoxic T Cell Function	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Bone Marrow Immunophenotyping	Normal
Epidermal Immune Composition	Normal

References

1. Chiang PW, Fogel E, Jackson CL, Lieuallen K, Lennon G, Qu X, Wang SQ, Kurnit DM (Dec 1996). "Isolation, sequencing, and mapping of the human homologue of the yeast transcription factor, SPT5". Genomics 38 (3): 421–4. doi:10.1006/geno.1996.0646. PMID 8975720.
2. "Entrez Gene: SUPT5H Suppressor of Ty 5 homolog (S. cerevisiae)".
3. Garber ME, Mayall TP, Suess EM, Meisenhelder J, Thompson NE, Jones KA (Sep 2000). "CDK9 autophosphorylation regulates high-affinity binding of the human immunodeficiency virus type 1 tat-P-TEFb complex to TAR RNA". Molecular and Cellular Biology 20 (18): 6958–69. doi:10.1128/mcb.20.18.6958-6969.2000. PMC 88771. PMID 10958691.
4. Kim JB, Yamaguchi Y, Wada T, Handa H, Sharp PA (Sep 1999). "Tat-SF1 protein associates with RAP30 and human SPT5 proteins". Molecular and Cellular Biology 19 (9): 5960–8. doi:10.1128/mcb.19.9.5960. PMC 84462. PMID 10454543.
5. Lavoie SB, Albert AL, Handa H, Vincent M, Bensaude O (Sep 2001). "The peptidyl-prolyl isomerase Pin1 interacts with hSpt5 phosphorylated by Cdk9". Journal of Molecular Biology 312 (4): 675–85. doi:10.1006/jmbi.2001.4991. PMID 11575923.
6. Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, Buratowski S, Handa H (Feb 1998). "DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs". Genes & Development 12 (3): 343–56. doi:10.1101/gad.12.3.343. PMC 316480. PMID 9450929.
7. Kwak YT, Guo J, Prajapati S, Park KJ, Surabhi RM, Miller B, Gehrig P, Gaynor RB (Apr 2003). "Methylation of SPT5 regulates its interaction with RNA polymerase II and transcriptional elongation properties". Molecular Cell 11 (4): 1055–66. doi:10.1016/s1097-2765(03)00101-1. PMID 12718890.
8. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
9. "International Mouse Phenotyping Consortium".
10. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
11. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
12. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
13. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
14. "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (Apr 1996). "A "double adaptor" method for improved shotgun library construction". Analytical Biochemistry 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA (Apr 1997). "Large-scale concatenation cDNA sequencing". Genome Research 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
• Stachora AA, Schäfer RE, Pohlmeier M, Maier G, Ponstingl H (Jun 1997). "Human Supt5h protein, a putative modulator of chromatin structure, is reversibly phosphorylated in mitosis". FEBS Letters 409 (1): 74–8. doi:10.1016/S0014-5793(97)00486-9. PMID 9199507.
• Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, Buratowski S, Handa H (Feb 1998). "DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs". Genes & Development 12 (3): 343–56. doi:10.1101/gad.12.3.343. PMC 316480. PMID 9450929.
• Wu-Baer F, Lane WS, Gaynor RB (Mar 1998). "Role of the human homolog of the yeast transcription factor SPT5 in HIV-1 Tat-activation". Journal of Molecular Biology 277 (2): 179–97. doi:10.1006/jmbi.1997.1601. PMID 9514752.
• Wada T, Takagi T, Yamaguchi Y, Watanabe D, Handa H (Dec 1998). "Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro". The EMBO Journal 17 (24): 7395–403. doi:10.1093/emboj/17.24.7395. PMC 1171084. PMID 9857195.
• Yamaguchi Y, Wada T, Watanabe D, Takagi T, Hasegawa J, Handa H (Mar 1999). "Structure and function of the human transcription elongation factor DSIF". The Journal of Biological Chemistry 274 (12): 8085–92. doi:10.1074/jbc.274.12.8085. PMID 10075709.
• Yamaguchi Y, Takagi T, Wada T, Yano K, Furuya A, Sugimoto S, Hasegawa J, Handa H (Apr 1999). "NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation". Cell 97 (1): 41–51. doi:10.1016/S0092-8674(00)80713-8. PMID 10199401.
• Parada CA, Roeder RG (Jul 1999). "A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription". The EMBO Journal 18 (13): 3688–701. doi:10.1093/emboj/18.13.3688. PMC 1171446. PMID 10393184.
• Wen Y, Shatkin AJ (Jul 1999). "Transcription elongation factor hSPT5 stimulates mRNA capping". Genes & Development 13 (14): 1774–9. doi:10.1101/gad.13.14.1774. PMC 316881. PMID 10421630.
• Kim JB, Yamaguchi Y, Wada T, Handa H, Sharp PA (Sep 1999). "Tat-SF1 protein associates with RAP30 and human SPT5 proteins". Molecular and Cellular Biology 19 (9): 5960–8. doi:10.1128/mcb.19.9.5960. PMC 84462. PMID 10454543.
• Ivanov D, Kwak YT, Guo J, Gaynor RB (May 2000). "Domains in the SPT5 protein that modulate its transcriptional regulatory properties". Molecular and Cellular Biology 20 (9): 2970–83. doi:10.1128/MCB.20.9.2970-2983.2000. PMC 85557. PMID 10757782.
• Wada T, Orphanides G, Hasegawa J, Kim DK, Shima D, Yamaguchi Y, Fukuda A, Hisatake K, Oh S, Reinberg D, Handa H (Jun 2000). "FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between P-TEFb and TFIIH". Molecular Cell 5 (6): 1067–72. doi:10.1016/S1097-2765(00)80272-5. PMID 10912001.
• Ping YH, Rana TM (Apr 2001). "DSIF and NELF interact with RNA polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation". The Journal of Biological Chemistry 276 (16): 12951–8. doi:10.1074/jbc.M006130200. PMID 11112772.
• Kim JB, Sharp PA (Apr 2001). "Positive transcription elongation factor B phosphorylates hSPT5 and RNA polymerase II carboxyl-terminal domain independently of cyclin-dependent kinase-activating kinase". The Journal of Biological Chemistry 276 (15): 12317–23. doi:10.1074/jbc.M010908200. PMID 11145967.
• Renner DB, Yamaguchi Y, Wada T, Handa H, Price DH (Nov 2001). "A highly purified RNA polymerase II elongation control system". The Journal of Biological Chemistry 276 (45): 42601–9. doi:10.1074/jbc.M104967200. PMID 11553615.
• Lavoie SB, Albert AL, Handa H, Vincent M, Bensaude O (Sep 2001). "The peptidyl-prolyl isomerase Pin1 interacts with hSpt5 phosphorylated by Cdk9". Journal of Molecular Biology 312 (4): 675–85. doi:10.1006/jmbi.2001.4991. PMID 11575923.
• Bourgeois CF, Kim YK, Churcher MJ, West MJ, Karn J (Feb 2002). "Spt5 cooperates with human immunodeficiency virus type 1 Tat by preventing premature RNA release at terminator sequences". Molecular and Cellular Biology 22 (4): 1079–93. doi:10.1128/MCB.22.4.1079-1093.2002. PMC 134635. PMID 11809800.
• Yamaguchi Y, Inukai N, Narita T, Wada T, Handa H (May 2002). "Evidence that negative elongation factor represses transcription elongation through binding to a DRB sensitivity-inducing factor/RNA polymerase II complex and RNA". Molecular and Cellular Biology 22 (9): 2918–27. doi:10.1128/MCB.22.9.2918-2927.2002. PMC 133766. PMID 11940650.