Steroid 15beta-monooxygenase

Steroid 15beta-monooxygenase (EC 1.14.15.8, cytochrome P-450meg, cytochrome P450meg, steroid 15beta-hydroxylase, CYP106A2, BmCYP106A2) is an enzyme with systematic name progesterone,reduced-ferredoxin:oxygen oxidoreductase (15beta-hydroxylating) .^[1]^[2]^[3]^[4]^[5] This enzyme catalyses the following chemical reaction

progesterone + reduced ferredoxin + O₂

\rightleftharpoons

15beta-hydroxyprogesterone + oxidized ferredoxin + H₂O

The enzyme from Bacillus megaterium hydroxylates a variety of 3-oxo-Delta4-steroids in position 15beta.

References

↑ Berg, A., Ingelman-Sundberg, M. and Gustafsson, J.A. (1979). "Purification and characterization of cytochrome P-450meg". J. Biol. Chem. 254 (12): 5264–5271. PMID 109432.
↑ Berg, A., Gustafsson, J.A. and Ingelman-Sundberg, M. (1976). "Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium". J. Biol. Chem. 251 (9): 2831–2838. PMID 177422.
↑ Lisurek, M., Kang, M.J., Hartmann, R.W. and Bernhardt, R. (2004). "Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry". Biochem. Biophys. Res. Commun. 319 (2): 677–682. doi:10.1016/j.bbrc.2004.05.037. PMID 15178459.
↑ Goni, G., Zollner, A., Lisurek, M., Velazquez-Campoy, A., Pinto, S., Gomez-Moreno, C., Hannemann, F., Bernhardt, R. and Medina, M. (2009). "Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368". Biochim. Biophys. Acta 1794 (11): 1635–1642. doi:10.1016/j.bbapap.2009.07.012. PMID 19635596.
↑ Lisurek, M., Simgen, B., Antes, I. and Bernhardt, R. (2008). "Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation". Chembiochem 9 (9): 1439–1449. doi:10.1002/cbic.200700670. PMID 18481342.

External links

Steroid 15beta-monooxygenase at the US National Library of Medicine Medical Subject Headings (MeSH)

Oxidoreductases: dioxygenases, including steroid hydroxylases (EC 1.14)

1.14.11: 2-oxoglutarate	Prolyl hydroxylase Lysyl hydroxylase

1.14.13: NADH or NADPH	Flavin-containing monooxygenase FMO1 FMO2 FMO3 FMO4 FMO5 Nitric oxide synthase NOS1 NOS2 NOS3 Cholesterol 7 alpha-hydroxylase Methane monooxygenase 3A4 Lanosterol 14 alpha-demethylase 24-hydroxycholesterol 7α-hydroxylase

1.14.14: reduced flavin or flavoprotein	19A1 2D6 2E1

1.14.15: reduced iron-sulfur protein	11B1 11B2 11A1

1.14.16: reduced pteridine (BH4 dependent)	Phenylalanine hydroxylase Tyrosine hydroxylase Tryptophan hydroxylase

1.14.17: reduced ascorbate	Dopamine beta-hydroxylase

1.14.18-19: other	Tyrosinase Stearoyl-CoA desaturase-1

1.14.99 - miscellaneous	Cyclooxygenase Heme oxygenase (HMOX1) Squalene monooxygenase 17A1 21A2

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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