TRNAIle-lysidine synthase
TRNAIle-lysidine synthase (EC 6.3.4.19, TilS, mesJ (gene), yacA (gene), isoleucine-specific transfer ribonucleate lysidine synthetase, tRNAIle-lysidine synthetase) is an enzyme with systematic name L-lysine:(tRNAIle2)-cytidine34 ligase (AMP-forming).[1][2][3][4][5] This enzyme catalyses the following chemical reaction
- [tRNAIle2]-cytidine34 + L-lysine + ATP
[tRNAIle2]-lysidine34 + AMP + diphosphate + H2O
The bacterial enzyme modifies the wobble base of the CAU anticodon of tRNAIle at the oxo group in position 2 of cytidine34.
References
- ↑ Ikeuchi, Y., Soma, A., Ote, T., Kato, J., Sekine, Y. and Suzuki, T. (2005). "molecular mechanism of lysidine synthesis that determines tRNA identity and codon recognition". Mol. Cell 19 (2): 235–246. doi:10.1016/j.molcel.2005.06.007. PMID 16039592.
- ↑ Salowe, S.P., Wiltsie, J., Hawkins, J.C. and Sonatore, L.M. (2009). "The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase". J. Biol. Chem. 284: 9656–9662. doi:10.1074/jbc.M809013200. PMC 2665086. PMID 19233850.
- ↑ Nakanishi, K., Fukai, S., Ikeuchi, Y., Soma, A., Sekine, Y., Suzuki, T. and Nureki, O. (2005). "Structural basis for lysidine formation by ATP pyrophosphatase accompanied by a lysine-specific loop and a tRNA-recognition domain". Proc. Natl. Acad. Sci. USA 102: 7487–7492. doi:10.1073/pnas.0501003102. PMC 1140429. PMID 15894617.
- ↑ Soma, A., Ikeuchi, Y., Kanemasa, S., Kobayashi, K., Ogasawara, N., Ote, T., Kato, J., Watanabe, K., Sekine, Y. and Suzuki, T. (2003). "An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA". Mol. Cell 12 (3): 689–698. doi:10.1016/s1097-2765(03)00346-0. PMID 14527414.
- ↑ Nakanishi, K., Bonnefond, L., Kimura, S., Suzuki, T., Ishitani, R. and Nureki, O. (2009). "Structural basis for translational fidelity ensured by transfer RNA lysidine synthetase". Nature 461 (7267): 1144–1148. doi:10.1038/nature08474. PMID 19847269.
External links
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| 6.1: Carbon-Oxygen | |
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| 6.2: Carbon-Sulfur | |
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| 6.3: Carbon-Nitrogen | |
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