TRNA (cytidine34-2'-O)-methyltransferase

tRNA (cytidine34-2'-O)-methyltransferase
Identifiers
EC number 2.1.1.207
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

tRNA (cytidine34-2'-O)-methyltransferase (EC 2.1.1.207, yibK (gene), methyltransferase yibK, TrmL, tRNA methyltransferase L, tRNA (cytidine34/5-carboxymethylaminomethyluridine34-2'-O)-methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (cytidine34/5-carboxymethylaminomethyluridine34-2'-O)-methyltransferase.[1] This enzyme catalyses the following chemical reaction

(1) S-adenosyl-L-methionine + cytidine34 in tRNA \rightleftharpoons S-adenosyl-L-homocysteine + 2'-O-methylcytidine34 in tRNA
(2) S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine34 in tRNALeu \rightleftharpoons S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNALeu

The enzyme from Escherichia coli catalyses the 2'-O-methylation of cytidine or 5-carboxymethylaminomethyluridine at the wobble position at nucleotide 34 in tRNALeuCmAA and tRNALeucmnm5UmAA.

References

  1. Benitez-Paez, A., Villarroya, M., Douthwaite, S., Gabaldon, T. and Armengod, M.E. (2010). "YibK is the 2-O-methyltransferase TrmL that modifies the wobble nucleotide in Escherichia coli tRNA(Leu) isoacceptors". RNA 16: 2131–2143. doi:10.1261/rna.2245910. PMID 20855540.

External links

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