Translin

Translin
PDB rendering based on 1j1j.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1J1J​, 3PJA​, 3QB5​, 4WYV​
Identifiers
Symbols	TSN ; BCLF-1; C3PO; RCHF1; REHF-1; TBRBP; TRSLN
External IDs	OMIM: 600575 MGI: 109263 HomoloGene: 3397 GeneCards: TSN Gene
Gene ontology Molecular function • DNA binding • single-stranded DNA binding • mRNA binding • endoribonuclease activity • protein binding • sequence-specific DNA binding Cellular component • nucleus • cytoplasm • cytosol Biological process • DNA recombination • gene expression • RNA phosphodiester bond hydrolysis, endonucleolytic Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	7247	22099
Ensembl	ENSG00000211460	ENSMUSG00000026374
UniProt	Q15631	Q62348
RefSeq (mRNA)	NM_001261401	NM_011650
RefSeq (protein)	NP_001248330	NP_035780
Location (UCSC)	Chr 2: 121.74 – 121.77 Mb	Chr 1: 118.29 – 118.31 Mb
PubMed search

Translin is a DNA-binding protein that in humans is encoded by the TSN gene.^[1][2][3] Together with translin-associated factor X, translin forms the component 3 of promoter of RISC (C3PO) complex which facilitates endonucleolytic cleavage of the passenger strand during microRNA loading into the RNA-induced silencing complex (RISC).^[4][5][6]

Function

This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms.^[3]

Interactions

Translin has been shown to interact with PPP1R15A.^[7]

References

1. Kasai M, Aoki K, Matsuo Y, Minowada J, Maziarz RT, Strominger JL (Jul 1994). "Recombination hotspot associated factors specifically recognize novel target sequences at the site of interchromosomal rearrangements in T-ALL patients with t(8;14)(q24;q11) and t(1;14)(p32;q11)". International Immunology 6 (7): 1017–25. doi:10.1093/intimm/6.7.1017. PMID 7947454.
2. Aoki K, Inazawa J, Takahashi T, Nakahara K, Kasai M (Jul 1997). "Genomic structure and chromosomal localization of the gene encoding translin, a recombination hotspot binding protein". Genomics 43 (2): 237–41. doi:10.1006/geno.1997.4796. PMID 9244443.
3. "Entrez Gene: TSN translin".
4. Liu Y, Ye X, Jiang F, Liang C, Chen D, Peng J, Kinch LN, Grishin NV, Liu Q (Aug 2009). "C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation". Science 325 (5941): 750–3. doi:10.1126/science.1176325. PMID 19661431.
5. Ye X, Huang N, Liu Y, Paroo Z, Huerta C, Li P, Chen S, Liu Q, Zhang H (Jun 2011). "Structure of C3PO and mechanism of human RISC activation". Nature Structural & Molecular Biology 18 (6): 650–7. doi:10.1038/nsmb.2032. PMID 21552258.
6. Ha M, Kim VN (Aug 2014). "Regulation of microRNA biogenesis". Nature Reviews Molecular Cell Biology 15 (8): 509–24. doi:10.1038/nrm3838. PMID 25027649.
7. Hasegawa T, Isobe K (Aug 1999). "Evidence for the interaction between Translin and GADD34 in mammalian cells". Biochimica et Biophysica Acta 1428 (2-3): 161–8. doi:10.1016/s0304-4165(99)00060-4. PMID 10434033.

Further reading

• Aoki K, Suzuki K, Sugano T, Tasaka T, Nakahara K, Kuge O, Omori A, Kasai M (Jun 1995). "A novel gene, Translin, encodes a recombination hotspot binding protein associated with chromosomal translocations". Nature Genetics 10 (2): 167–74. doi:10.1038/ng0695-167. PMID 7663511.
• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Aoki K, Nakahara K, Ikegawa C, Seto M, Takahashi T, Minowada J, Strominger JL, Maziarz RT, Kasai M (Apr 1994). "Nuclear proteins binding to a novel target sequence within the recombination hotspot regions of Bcl-2 and the immunoglobulin DH gene family". Oncogene 9 (4): 1109–15. PMID 8134113.
• Aoki K, Ishida R, Kasai M (Jan 1997). "Isolation and characterization of a cDNA encoding a Translin-like protein, TRAX". FEBS Letters 401 (2-3): 109–12. doi:10.1016/S0014-5793(96)01444-5. PMID 9013868.
• Kasai M, Matsuzaki T, Katayanagi K, Omori A, Maziarz RT, Strominger JL, Aoki K, Suzuki K (Apr 1997). "The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome". The Journal of Biological Chemistry 272 (17): 11402–7. doi:10.1074/jbc.272.17.11402. PMID 9111049.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Hasegawa T, Isobe K (Aug 1999). "Evidence for the interaction between Translin and GADD34 in mammalian cells". Biochimica et Biophysica Acta 1428 (2-3): 161–8. doi:10.1016/s0304-4165(99)00060-4. PMID 10434033.
• Badge RM, Yardley J, Jeffreys AJ, Armour JA (May 2000). "Crossover breakpoint mapping identifies a subtelomeric hotspot for male meiotic recombination". Human Molecular Genetics 9 (8): 1239–44. doi:10.1093/hmg/9.8.1239. PMID 10767349.
• Hosaka T, Kanoe H, Nakayama T, Murakami H, Yamamoto H, Nakamata T, Tsuboyama T, Oka M, Kasai M, Sasaki MS, Nakamura T, Toguchida J (Nov 2000). "Translin binds to the sequences adjacent to the breakpoints of the TLS and CHOP genes in liposarcomas with translocation t(12;6)". Oncogene 19 (50): 5821–5. doi:10.1038/sj.onc.1203943. PMID 11126370.
• Chennathukuzhi VM, Kurihara Y, Bray JD, Hecht NB (Apr 2001). "Trax (translin-associated factor X), a primarily cytoplasmic protein, inhibits the binding of TB-RBP (translin) to RNA". The Journal of Biological Chemistry 276 (16): 13256–63. doi:10.1074/jbc.M009707200. PMID 11278549.
• Lee SP, Fuior E, Lewis MS, Han MK (Nov 2001). "Analytical ultracentrifugation studies of translin: analysis of protein-DNA interactions using a single-stranded fluorogenic oligonucleotide". Biochemistry 40 (46): 14081–8. doi:10.1021/bi010302t. PMID 11705401.
• Erdemir T, Bilican B, Oncel D, Goding CR, Yavuzer U (Jan 2002). "DNA damage-dependent interaction of the nuclear matrix protein C1D with Translin-associated factor X (TRAX)". Journal of Cell Science 115 (Pt 1): 207–16. PMID 11801738.
• Han MK, Lin P, Paek D, Harvey JJ, Fuior E, Knutson JR (Mar 2002). "Fluorescence studies of pyrene maleimide-labeled translin: excimer fluorescence indicates subunits associate in a tail-to-tail configuration to form octamer". Biochemistry 41 (10): 3468–76. doi:10.1021/bi015901e. PMID 11876655.
• Sengupta K, Rao BJ (Dec 2002). "Translin binding to DNA: recruitment through DNA ends and consequent conformational transitions". Biochemistry 41 (51): 15315–26. doi:10.1021/bi026378m. PMID 12484770.
• Li K, Wang L, Cheng J, Lu YY, Zhang LX, Mu JS, Hong Y, Liu Y, Duan HJ, Wang G, Li L, Chen JM (Feb 2003). "Interaction between hepatitis C virus core protein and translin protein--a possible molecular mechanism for hepatocellular carcinoma and lymphoma caused by hepatitis C virus". World Journal of Gastroenterology 9 (2): 300–3. PMID 12532453.
• Yang S, Cho YS, Chennathukuzhi VM, Underkoffler LA, Loomes K, Hecht NB (Mar 2004). "Translin-associated factor X is post-transcriptionally regulated by its partner protein TB-RBP, and both are essential for normal cell proliferation". The Journal of Biological Chemistry 279 (13): 12605–14. doi:10.1074/jbc.M313133200. PMID 14711818.