USP11

Ubiquitin specific peptidase 11
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 4MEL​
Identifiers
Symbols	USP11 ; UHX1
External IDs	OMIM: 300050 MGI: 2384312 HomoloGene: 31252 GeneCards: USP11 Gene
EC number	3.4.19.12
Gene ontology Molecular function • cysteine-type endopeptidase activity • ubiquitin-specific protease activity • protein binding Cellular component • nucleus • cytoplasm Biological process • protein deubiquitination • proteasome-mediated ubiquitin-dependent protein catabolic process • regulation of proteasomal protein catabolic process Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	8237	236733
Ensembl	ENSG00000102226	ENSMUSG00000031066
UniProt	P51784	Q99K46
RefSeq (mRNA)	NM_004651	NM_145628
RefSeq (protein)	NP_004642	NP_663603
Location (UCSC)	Chr X: 47.23 – 47.25 Mb	Chr X: 20.7 – 20.72 Mb
PubMed search

Ubiquitin carboxyl-terminal hydrolase 11 is an enzyme that in humans is encoded by the USP11 gene.^[1][2]

Function

Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This gene encodes a deubiquitinating enzyme which lies in a gene cluster on chromosome Xp11.23^[2]

Interactions

USP11 has been shown to interact with RANBP9.^[3]

Model organisms

Model organisms have been used in the study of USP11 function. A conditional knockout mouse line called Usp11^{tm1(KOMP)Wtsi} was generated at the Wellcome Trust Sanger Institute.^[4] Male and female animals underwent a standardized phenotypic screen^[5] to determine the effects of deletion.^[6][7][8][9] Additional screens performed: - In-depth immunological phenotyping^[10]

Usp11 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[5][10]
Insulin	Normal
Homozygous viability at P14	Normal
Homozygous Fertility	Normal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Epidermal Immune Composition	Normal
Influenza Challenge	Normal

References

1. Puente XS, Sánchez LM, Overall CM, López-Otín C (Jul 2003). "Human and mouse proteases: a comparative genomic approach". Nature Reviews. Genetics 4 (7): 544–58. doi:10.1038/nrg1111. PMID 12838346.
2. "Entrez Gene: USP11 ubiquitin specific peptidase 11".
3. Ideguchi H, Ueda A, Tanaka M, Yang J, Tsuji T, Ohno S, Hagiwara E, Aoki A, Ishigatsubo Y (Oct 2002). "Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM". The Biochemical Journal 367 (Pt 1): 87–95. doi:10.1042/BJ20011851. PMC 1222860. PMID 12084015.
4. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
5. "International Mouse Phenotyping Consortium".
6. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
7. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
8. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
9. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
10. "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

• D'Andrea A, Pellman D (1999). "Deubiquitinating enzymes: a new class of biological regulators". Critical Reviews in Biochemistry and Molecular Biology 33 (5): 337–52. doi:10.1080/10409239891204251. PMID 9827704.
• Swanson DA, Freund CL, Ploder L, McInnes RR, Valle D (Apr 1996). "A ubiquitin C-terminal hydrolase gene on the proximal short arm of the X chromosome: implications for X-linked retinal disorders". Human Molecular Genetics 5 (4): 533–8. doi:10.1093/hmg/5.4.533. PMID 8845848.
• Brandau O, Nyakatura G, Jedele KB, Platzer M, Achatz H, Ross M, Murken J, Rosenthal A, Meindl A (1999). "UHX1 and PCTK1: precise characterisation and localisation within a gene-rich region in Xp11.23 and evaluation as candidate genes for retinal diseases mapped to Xp21.1-p11.2". European Journal of Human Genetics 6 (5): 459–66. doi:10.1038/sj.ejhg.5200207. PMID 9801870.
• Stoddart KL, Jermak C, Nagaraja R, Schlessinger D, Bech-Hansen NT (Feb 1999). "Physical map covering a 2 Mb region in human xp11.3 distal to DX6849". Gene 227 (1): 111–6. doi:10.1016/S0378-1119(98)00564-2. PMID 9931462.
• Thiselton DL, McDowall J, Brandau O, Ramser J, d'Esposito F, Bhattacharya SS, Ross MT, Hardcastle AJ, Meindl A (Apr 2002). "An integrated, functionally annotated gene map of the DXS8026-ELK1 interval on human Xp11.3-Xp11.23: potential hotspot for neurogenetic disorders". Genomics 79 (4): 560–72. doi:10.1006/geno.2002.6733. PMID 11944989.
• Ideguchi H, Ueda A, Tanaka M, Yang J, Tsuji T, Ohno S, Hagiwara E, Aoki A, Ishigatsubo Y (Oct 2002). "Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM". The Biochemical Journal 367 (Pt 1): 87–95. doi:10.1042/BJ20011851. PMC 1222860. PMID 12084015.
• Angelats C, Wang XW, Jermiin LS, Copeland NG, Jenkins NA, Baker RT (Jan 2003). "Isolation and characterization of the mouse ubiquitin-specific protease Usp15". Mammalian Genome 14 (1): 31–46. doi:10.1007/s00335-002-3035-0. PMID 12532266.
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (Feb 2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nature Cell Biology 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216.
• Schoenfeld AR, Apgar S, Dolios G, Wang R, Aaronson SA (Sep 2004). "BRCA2 is ubiquitinated in vivo and interacts with USP11, a deubiquitinating enzyme that exhibits prosurvival function in the cellular response to DNA damage". Molecular and Cellular Biology 24 (17): 7444–55. doi:10.1128/MCB.24.17.7444-7455.2004. PMC 506974. PMID 15314155.
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.