Vesicle-associated membrane protein 8

Vesicle-associated membrane protein 8
PDB rendering based on 1gl2.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 4WY4​
Identifiers
Symbols	VAMP8 ; EDB; VAMP-8
External IDs	OMIM: 603177 MGI: 1336882 HomoloGene: 37846 GeneCards: VAMP8 Gene
Gene ontology Molecular function • SNARE binding • SNAP receptor activity • protein binding • chloride channel inhibitor activity Cellular component • cytoplasm • lysosomal membrane • early endosome • cytosol • plasma membrane • membrane • integral component of membrane • secretory granule membrane • SNARE complex • early endosome membrane • late endosome membrane • vesicle • azurophil granule membrane • perinuclear region of cytoplasm • recycling endosome • extracellular exosome • mucin granule Biological process • post-Golgi vesicle-mediated transport • vesicle fusion • protein transport • eosinophil degranulation • neutrophil degranulation • viral entry into host cell • membrane organization • mucus secretion • autophagosome maturation • regulation of protein localization to plasma membrane • negative regulation of secretion by cell • positive regulation of histamine secretion by mast cell Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	8673	22320
Ensembl	ENSG00000118640	ENSMUSG00000050732
UniProt	Q9BV40	O70404
RefSeq (mRNA)	NM_003761	NM_016794
RefSeq (protein)	NP_003752	NP_058074
Location (UCSC)	Chr 2: 85.56 – 85.58 Mb	Chr 6: 72.39 – 72.39 Mb
PubMed search

Vesicle-associated membrane protein 8 is a protein that in humans is encoded by the VAMP8 gene.^[1][2][3]

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex.^[3]

Interactions

Vesicle-associated membrane protein 8 has been shown to interact with STX4,^[4][5] SNAP23,^[4][6] STX1A,^[7] STX8^[8] and STX7.^[8][9]

References

1. Bui TD, Wong SH, Lu L, Hong W (February 1999). "Endobrevin maps to chromosome 2 in human and chromosome 6 in mouse". Genomics 54 (3): 579–80. doi:10.1006/geno.1998.5596. PMID 9878266.
2. Wong SH, Zhang T, Xu Y, Subramaniam VN, Griffiths G, Hong W (July 1998). "Endobrevin, a Novel Synaptobrevin/VAMP-Like Protein Preferentially Associated with the Early Endosome". Mol Biol Cell 9 (6): 1549–63. doi:10.1091/mbc.9.6.1549. PMC 25382. PMID 9614193.
3. "Entrez Gene: VAMP8 vesicle-associated membrane protein 8 (endobrevin)".
4. Paumet, F; Le Mao J; Martin S; Galli T; David B; Blank U; Roa M (June 2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". J. Immunol. (UNITED STATES) 164 (11): 5850–7. doi:10.4049/jimmunol.164.11.5850. ISSN 0022-1767. PMID 10820264.
5. Polgár, János; Chung Sul-Hee; Reed Guy L (August 2002). "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion". Blood (United States) 100 (3): 1081–3. doi:10.1182/blood.V100.3.1081. ISSN 0006-4971. PMID 12130530.
6. Imai, Akane; Nashida Tomoko; Yoshie Sumio; Shimomura Hiromi (August 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Arch. Oral Biol. (England) 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. ISSN 0003-9969. PMID 12828989.
7. Nagamatsu, S; Nakamichi Y; Watanabe T; Matsushima S; Yamaguchi S; Ni J; Itagaki E; Ishida H (January 2001). "Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules". J. Cell. Sci. (England) 114 (Pt 1): 219–227. ISSN 0021-9533. PMID 11112705.
8. Antonin, W; Holroyd C; Fasshauer D; Pabst S; Von Mollard G F; Jahn R (Dec 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. (ENGLAND) 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. ISSN 0261-4189. PMC 305878. PMID 11101518.
9. Wade, N; Bryant N J; Connolly L M; Simpson R J; Luzio J P; Piper R C; James D E (June 2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". J. Biol. Chem. (United States) 276 (23): 19820–7. doi:10.1074/jbc.M010838200. ISSN 0021-9258. PMID 11278762.

Further reading

• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Fasshauer D, Antonin W, Margittai M, et al. (1999). "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties". J. Biol. Chem. 274 (22): 15440–6. doi:10.1074/jbc.274.22.15440. PMID 10336434.
• Kim PK, Hollerbach C, Trimble WS, et al. (2000). "Identification of the endoplasmic reticulum targeting signal in vesicle-associated membrane proteins". J. Biol. Chem. 274 (52): 36876–82. doi:10.1074/jbc.274.52.36876. PMID 10601239.
• Paumet F, Le Mao J, Martin S, et al. (2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". J. Immunol. 164 (11): 5850–7. doi:10.4049/jimmunol.164.11.5850. PMID 10820264.
• Antonin W, Holroyd C, Tikkanen R, et al. (2000). "The R-SNARE Endobrevin/VAMP-8 Mediates Homotypic Fusion of Early Endosomes and Late Endosomes". Mol. Biol. Cell 11 (10): 3289–98. doi:10.1091/mbc.11.10.3289. PMC 14992. PMID 11029036.
• Antonin W, Holroyd C, Fasshauer D, et al. (2001). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. PMC 305878. PMID 11101518.
• Nagamatsu S, Nakamichi Y, Watanabe T, et al. (2001). "Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules". J. Cell. Sci. 114 (Pt 1): 219–227. PMID 11112705.
• Wade N, Bryant NJ, Connolly LM, et al. (2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". J. Biol. Chem. 276 (23): 19820–7. doi:10.1074/jbc.M010838200. PMID 11278762.
• Antonin W, Fasshauer D, Becker S, et al. (2002). "Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs". Nat. Struct. Biol. 9 (2): 107–11. doi:10.1038/nsb746. PMID 11786915.
• Polgár J, Chung SH, Reed GL (2002). "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion". Blood 100 (3): 1081–3. doi:10.1182/blood.V100.3.1081. PMID 12130530.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Imai A, Nashida T, Yoshie S, Shimomura H (2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Arch. Oral Biol. 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. PMID 12828989.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature 434 (7034): 724–31. doi:10.1038/nature03466. PMID 15815621.
• Brinkman JF, Ottenheim CP, de Jong LA, et al. (2006). "VAMP5 and VAMP8 are most likely not involved in primary open-angle glaucoma". Mol. Vis. 11: 582–6. PMID 16110299.
• Oishi Y, Arakawa T, Tanimura A, et al. (2007). "Role of VAMP-2, VAMP-7, and VAMP-8 in constitutive exocytosis from HSY cells". Histochem. Cell Biol. 125 (3): 273–81. doi:10.1007/s00418-005-0068-y. PMID 16195891.
• Shiffman D, Rowland CM, Louie JZ, et al. (2006). "Gene variants of VAMP8 and HNRPUL1 are associated with early-onset myocardial infarction". Arterioscler. Thromb. Vasc. Biol. 26 (7): 1613–8. doi:10.1161/01.ATV.0000226543.77214.e4. PMID 16690874.