Vitamin D3 24-hydroxylase

Vitamin D3 24-hydroxylase
Identifiers
EC number	1.14.13.126
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Vitamin D3 24-hydroxylase (EC 1.14.13.126, CYP24A1) is an enzyme with systematic name calcitriol,NADPH:oxygen oxidoreductase (24-hydroxylating).^{[1][2][3][4][5][6][7]} This enzyme catalyses the following chemical reaction

(1) calcitriol + NADPH + H⁺ + O₂ $\rightleftharpoons$ calcitetrol + NADP⁺ + H₂O

(2) calcidiol + NADPH + H⁺ + O₂ $\rightleftharpoons$ secalciferol + NADP⁺ + H₂O

Vitamin D3 24-hydroxylase is a heme-thiolate enzyme (P-450).

References

1. Masuda, S., Strugnell, S.A., Knutson, J.C., St-Arnaud, R. and Jones, G. (2006). "Evidence for the activation of 1α-hydroxyvitamin D₂ by 25-hydroxyvitamin D-24-hydroxylase: delineation of pathways involving 1α,24-dihydroxyvitamin D₂ and 1α,25-dihydroxyvitamin D₂". Biochim. Biophys. Acta 1761 (2): 221–234. doi:10.1016/j.bbalip.2006.01.004. PMID 16516540.
2. Hamamoto, H., Kusudo, T., Urushino, N., Masuno, H., Yamamoto, K., Yamada, S., Kamakura, M., Ohta, M., Inouye, K. and Sakaki, T. (2006). "Structure-function analysis of vitamin D 24-hydroxylase (CYP24A1) by site-directed mutagenesis: amino acid residues responsible for species-based difference of CYP24A1 between humans and rats". Mol. Pharmacol. 70 (1): 120–128. doi:10.1124/mol.106.023275. PMID 16617161.
3. Sakaki, T., Kagawa, N., Yamamoto, K. and Inouye, K. (2005). "Metabolism of vitamin D₃ by cytochromes P₄₅₀". Front. Biosci. 10: 119–134. doi:10.2741/1514. PMID 15574355.
4. Prosser, D.E., Kaufmann, M., O'Leary, B., Byford, V. and Jones, G. (2007). "Single A326G mutation converts human CYP24A1 from 25-OH-D3-24-hydroxylase into -23-hydroxylase, generating 1α,25-(OH)₂D₃-26,23-lactone". Proc. Natl. Acad. Sci. USA 104 (31): 12673–12678. doi:10.1073/pnas.0702093104. PMC 1937525. PMID 17646648.
5. Kusudo, T., Sakaki, T., Abe, D., Fujishima, T., Kittaka, A., Takayama, H., Hatakeyama, S., Ohta, M. and Inouye, K. (2004). "Metabolism of A-ring diastereomers of 1α,25-dihydroxyvitamin D₃ by CYP24A1". Biochem. Biophys. Res. Commun. 321: 774–782. doi:10.1016/j.bbrc.2004.07.040. PMID 15358094.
6. Sawada, N., Kusudo, T., Sakaki, T., Hatakeyama, S., Hanada, M., Abe, D., Kamao, M., Okano, T., Ohta, M. and Inouye, K. (2004). "Novel metabolism of 1 α,25-dihydroxyvitamin D₃ with C₂₄-C₂₅ bond cleavage catalyzed by human CYP24A1". Biochemistry 43: 4530–4537. doi:10.1021/bi030207f. PMID 15078099.
7. Prosser, D.E. and Jones, G. (2004). "Enzymes involved in the activation and inactivation of vitamin D". Trends Biochem. Sci. 29 (12): 664–673. doi:10.1016/j.tibs.2004.10.005. PMID 15544953.

External links

• Vitamin D3 24-hydroxylase at the US National Library of Medicine Medical Subject Headings (MeSH)