YdaO/yuaA leader

ydaO/yuaA leader
Predicted secondary structure and sequence conservation of ydaO-yuaA
Identifiers
Symbol ydaO-yuaA
Rfam RF00379
Other data
RNA type Cis-reg
Domain(s) Bacteria
SO 0000233

The YdaO/YuaA leader is a conserved RNA structure found upstream of the ydaO and yuaA genes in Bacillus subtilis and related genes in other bacteria. The ydaO/yuaA element is thought to act as a genetic "off" switch for the ydaO and yuaA genes in its native state. It is believed that the element may be triggered during osmotic shock leading to activation of ydaO, a predicted amino acid transporter gene, and members of the yuaA-yubG operon which code for KtrA and KtrB K+ transporters.[1]

Function

Further work has established that the ydaO element is widespread and is associated with a diverse set of genes which it controls in a sequence and structure-dependent fashion. The natural ligand is present in cells grown a defined media and transposon-directed knockouts result in disregulation of associated genes.[2]

A biochemical screen in Bacillus subtilis has shown that the ydaO motif specifically binds ATP and regulates the expression of genes in response to ATP. Thus demonstrating a role regulation of gene expression in response to energy balance in bacteria.[3]

References

  1. ↑ Barrick, JE; Corbino KA; Winkler WC; Nahvi A; Mandal M; Collins J; Lee M; Roth A; Sudarsan N; Jona I; Wickiser JK; Breaker RR (2004). "New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control". Proc Natl Acad Sci USA 101 (17): 6421–6426. doi:10.1073/pnas.0308014101. PMC 404060. PMID 15096624.
  2. ↑ Block KF, Hammond MC, Breaker RR (2010). "Evidence for Widespread Gene Control Function by the ydaO Riboswitch Candidate". J Bacteriol 192 (15): 3983–9. doi:10.1128/JB.00450-10. PMC 2916388. PMID 20511502.
  3. ↑ Watson PY, Fedor MJ (2012). "The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis.". Nat Chem Biol 8 (12): 963–5. doi:10.1038/nchembio.1095. PMID 23086297.

External links


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