| | Home team | Score | Away team | Date |
| 1 | 93rd Highland Regiment | 7–0 | Luton Town | 000000001890-10-04-00004 October 1890 |
| 2 | Ardwick | 12–0 | Liverpool Stanley | 000000001890-10-04-00004 October 1890 |
| 3 | Bishop Auckland | 3–0 | Rendel | 000000001890-10-04-00004 October 1890 |
| 4 | Boston | 3–0 | Eckington Works | 000000001890-10-04-00004 October 1890 |
| 5 | Carlisle | 1–6 | Bootle | 000000001890-10-04-00004 October 1890 |
| 6 | Chatham | 1–2 | Crusaders | 000000001890-10-04-00004 October 1890 |
| 7 | Chesham | 1–2 | Watford Rovers | 000000001890-10-04-00004 October 1890 |
| 8 | Chester St Oswald's | 0–6 | Chester | 000000001890-10-04-00004 October 1890 |
| 9 | City Ramblers | W–W | Crystal Palace Engineers | — |
| 10 | Clifton | 1–7 | Wednesbury Old Athletic | 000000001890-10-04-00004 October 1890 |
| 11 | Cliftonville | 4–3 | Macclesfield Town | 000000001890-10-04-00004 October 1890 |
| 12 | Clinton | 3–7 | Jardines | 000000001890-10-04-00004 October 1890 |
| 13 | Darlington St Augustine's | 3–2 | Barnard Castle Athleitc | 000000001890-10-04-00004 October 1890 |
| 14 | Darlington West End | 3–2 | Darlington St Hilda's | 000000001890-10-04-00004 October 1890 |
| 15 | Derby Junction | 0–1 | Sheffield United | 000000001890-10-04-00004 October 1890 |
| 16 | Ecclesfield | 8–2 | Grimsby Town | 000000001890-10-04-00004 October 1890 |
| 17 | Elswick Rangers | 2–5 | Newcastle West End | 000000001890-10-04-00004 October 1890 |
| 18 | Folkestone | 2–1 | Old Wykehamists | 000000001890-10-04-00004 October 1890 |
| 19 | Gainsborough Trinity | 1–3 | Lincoln City | 000000001890-10-04-00004 October 1890 |
| 20 | Gateshead Association | 2–8 | Sunderland Albion | 000000001890-10-04-00004 October 1890 |
| 21 | Gorton Villa | 4–1 | Denton | 000000001890-10-04-00004 October 1890 |
| 22 | Gravesend | 4–1 | Old Harrovians | 000000001890-10-04-00004 October 1890 |
| 23 | Hunts County | 3–1 | Norwich CEYMS | 000000001890-10-04-00004 October 1890 |
| 24 | Hurworth | 0–14 | Stockton | 000000001890-10-04-00004 October 1890 |
| 25 | Ipswich Town | 2–0 | Reading | 000000001890-10-04-00004 October 1890 |
| 26 | Kidderminster | 4–1 | Bath City | 000000001890-10-04-00004 October 1890 |
| 27 | Langley Green Victoria | 5–0 | Kettering Town | 000000001890-10-04-00004 October 1890 |
| 28 | Leicester Fosse | 0–4 | Burton Wanderers | 000000001890-10-04-00004 October 1890 |
| 29 | Long Eaton Rangers | W–W | Heeley | — |
| 30 | Matlock Town | W–W | Leys Recreation | — |
| 31 | Middlesbrough | 11–0 | Scarborough | 000000001890-10-04-00004 October 1890 |
| 32 | Milwall Athletic | 2–3 | Ilford | 000000001890-10-04-00004 October 1890 |
| 33 | Morpeth Harriers | 0–2 | Whitburn | 000000001890-10-04-00004 October 1890 |
| 34 | Nantwich | 5–4 | Linfield Athletic | 000000001890-10-04-00004 October 1890 |
| 35 | Newark | 4–0 | Notts Olympic | 000000001890-10-04-00004 October 1890 |
| 36 | Newton Heath | 2–0 | Higher Walton | 000000001890-10-04-00004 October 1890 |
| 37 | Northwich Victoria | 3–2 | Chirk | 000000001890-10-04-00004 October 1890 |
| 38 | Old Brightonians | 3–4 | Old Carthusians | 000000001890-10-04-00004 October 1890 |
| 39 | Old Etonians | 4–1 | Swifts | 000000001890-10-04-00004 October 1890 |
| 40 | Old St Mark's | 2–3 | London Caledonians | 000000001890-10-04-00004 October 1890 |
| 41 | Oldbury Town | 3–1 | Wellington St George's | 000000001890-10-04-00004 October 1890 |
| 42 | Over Wanderers | 2–3 | Druids | 000000001890-10-04-00004 October 1890 |
| 43 | Port Clarence | 4–1 | Whitby | 000000001890-10-04-00004 October 1890 |
| 44 | Rhosllanerchrugog | W–W | Shrewsbury Town | — |
| 45 | Rotherham Swifts | 5–2 | Attercliffe | 000000001890-10-04-00004 October 1890 |
| 46 | Rotherham Town | 13–0 | Sheffield | 000000001890-10-04-00004 October 1890 |
| 47 | Shankhouse | 5–4 (Void) | Birtley | 000000001890-10-04-00004 October 1890 |
| 47r | Shankhouse | 5–1 | Birtley | 000000001890-10-18-000018 October 1890 |
| 48 | Small Heath | 8–0 | Hednesford Town | 000000001890-10-04-00004 October 1890 |
| 49 | Southwick | 6–0 | Bishop Auckland Church Institute | 000000001890-10-04-00004 October 1890 |
| 50 | Staveley | 19–0 | Sheffield Walkley | 000000001890-10-04-00004 October 1890 |
| 51 | Swindon Town | 9–0 | Maidenhead | 000000001890-10-04-00004 October 1890 |
| 52 | Warmley | 0–12 | Walsall Town Swifts | 000000001890-10-04-00004 October 1890 |
| 53 | Warwick County | 1–3 | Burslem Port Vale | 000000001890-10-04-00004 October 1890 |
| 54 | Windsor Phoenix Athletic | 6–1 | Schorne College | 000000001890-10-04-00004 October 1890 |
| 55 | Witton | 1–4 | Heywood Central | 000000001890-10-04-00004 October 1890 |
| 56 | Wolverton | 0–4 | Marlow | 000000001890-10-04-00004 October 1890 |
| 57 | Workington | W–W | Clitheroe | — |