| | Home team | Score | Away team | Date |
| 1 | Fairfield[1] | 3–1 | South Shore | 000000001891-10-03-00003 October 1891 |
| 2 | Darlington | 3–0 | Port Clarence | 000000001891-10-03-00003 October 1891 |
| 3 | Bury | 3–1 | Witton | 000000001891-10-03-00003 October 1891 |
| 4 | Matlock | 4–2 | Beeston | 000000001891-10-03-00003 October 1891 |
| 5 | Reading | 2–1 | Newbury | 000000001891-10-03-00003 October 1891 |
| 6 | Shankhouse[2] | w–w | Birtley | |
| 7 | Folkestone | 3–3 | Old St Mark's | 000000001891-10-03-00003 October 1891 |
| 7R | Old St Mark's | 4–2 | Folkestone | 000000001891-10-10-000010 October 1891 |
| 8 | Maidenhead | 0–5 | Watford Rovers | 000000001891-10-03-00003 October 1891 |
| 9 | Old Etonians | 6–1 | Hunts County | 000000001891-10-03-00003 October 1891 |
| 10 | Rochester | 1–2 | Millwall Athletic | 000000001891-10-03-00003 October 1891 |
| 11 | Staveley | 3–0 | Notts Olympic | 000000001891-10-03-00003 October 1891 |
| 12 | Grimsby Town | 3–0 | Long Eaton Rangers | 000000001891-10-03-00003 October 1891 |
| 13 | Macclesfield Town | 4–2 | Newtown | 000000001891-10-03-00003 October 1891 |
| 14 | Old Westminsters | 5–1 | Ipswich Town | 000000001891-10-03-00003 October 1891 |
| 15 | Newark | 3–1 | Eckington Works | 000000001891-10-03-00003 October 1891 |
| 16 | Casuals | 3–2 | Clapton | 000000001891-10-03-00003 October 1891 |
| 17 | Lincoln City | 3–2 | Doncaster Rovers | 000000001891-10-03-00003 October 1891 |
| 18 | Higher Walton | 4–5 | Blackpool | 000000001891-10-03-00003 October 1891 |
| 19 | Burslem Port Vale | 2–4 | Burton Wanderers | 000000001891-10-03-00003 October 1891 |
| 20 | Luton Town | 4–3 | Swindon Town | 000000001891-10-03-00003 October 1891 |
| 21 | Gainsborough Trinity | 3–0 | Grantham | 000000001891-10-03-00003 October 1891 |
| 23 | Attercliffe | 1–2 | Rotherham Town | 000000001891-10-03-00003 October 1891 |
| 24 | London Caledonians | 4–2 | Chesham | 000000001891-10-03-00003 October 1891 |
| 25 | Newton Heath | 5–1 | Ardwick | 000000001891-10-03-00003 October 1891 |
| 26 | Scarborough | 4–1 | West Hartlepool Rovers | 000000001891-10-03-00003 October 1891 |
| 27 | Boston | 5–3 | Sheffield | 000000001891-10-03-00003 October 1891 |
| 28 | Whitburn | 3–2 | Sunderland Olympic | 000000001891-10-03-00003 October 1891 |
| 29 | Heywood Central[3] | w–w | Moss Bay | |
| 30 | Nantwich | 3–0 | Chester St Oswald's | 000000001891-10-03-00003 October 1891 |
| 31 | Gorton Villa | 6–1 | Leek | 000000001891-10-03-00003 October 1891 |
| 32 | Walsall Town Swifts | 7–2 | Wednesbury Old Athletic | 000000001891-10-03-00003 October 1891 |
| 33 | Walsall Town Swifts | 7–2 | Wednesbury Old Athletic | 000000001891-10-03-00003 October 1891 |
| 34 | Norwich Thorpe | 1–3 | Old Wykehamists | 000000001891-10-03-00003 October 1891 |
| 35 | Norwich CEYMS | 1–9 | Marlow | 000000001891-10-03-00003 October 1891 |
| 36 | Gateshead NER | 7–2 | Ashington | 000000001891-10-03-00003 October 1891 |
| 37 | Bishop Auckland | 4–3 | Southwick | 000000001891-10-03-00003 October 1891 |
| 38 | Darlington St Augustines | 3–4 | South Bank | 000000001891-10-03-00003 October 1891 |
| 39 | Clifton | 5–0 | Poole | 000000001891-10-03-00003 October 1891 |
| 40 | Leicester Fosse | 2–6 | Small Heath | 000000001891-10-03-00003 October 1891 |
| 41 | Hednesford Town | 1–2 | Brierley Hill Alliance | 000000001891-10-03-00003 October 1891 |
| 42 | Middlesbrough Ironopolis[4] | w–w | Hurworth | |
| 43 | Rhosllanerchrugog | 0–1 | Chester | 000000001891-10-03-00003 October 1891 |
| 44 | Ilford | 0–1 | Chatham | 000000001891-10-03-00003 October 1891 |
| 45 | Mansfield Town | 3–0 | Riddings | 000000001891-10-03-00003 October 1891 |
| 46 | Grantham Rovers[5] | w–w | Sheffield Heeley | |
| 47 | Warmley | 1–4 | Southampton St Mary's | 000000001891-10-03-00003 October 1891 |
| 48 | Wolverton[6] | w–w | Swifts | |
| 49 | Hereford[7] | w–w | Kettering | |
| 50 | Spennymoor | 4–3 | Whitby[8] | 000000001891-10-03-00003 October 1891 |
| 51 | Tow Law | 1–5 | Newcastle East End | 000000001891-10-03-00003 October 1891 |
| 52 | Prescot | 1–7 | Crewe Alexandra | 000000001891-10-03-00003 October 1891 |
| 53 | 1st Highland Light Infantry | 7–0 | Maidstone | 000000001891-10-03-00003 October 1891 |
| 54 | Old Cranleighans | 2–3 | Gravesend | 000000001891-10-03-00003 October 1891 |
| 55 | Crouch End | 6–2 | Ashford United | 000000001891-10-03-00003 October 1891 |
| 56 | Royton | 6–3 | Halliwell | 000000001891-10-03-00003 October 1891 |
| 57 | Somerset Rovers | 6–3 | Bristol St George | 000000001891-10-03-00003 October 1891 |
| 58 | Barrow | 1–3 | Fleetwood Rangers | 000000001891-10-03-00003 October 1891 |
| 59 | Tranmere Rovers | 1–5 | Northwich Victoria | 000000001891-10-03-00003 October 1891 |
| 60 | Greenhalgh's | 1–5 | Heanor Town | 000000001891-10-03-00003 October 1891 |
| 61 | Heywood | 6–0 | North Meols | 000000001891-10-03-00003 October 1891 |