AKAP3

A kinase (PRKA) anchor protein 3
Identifiers
Symbols	AKAP3 ; AKAP 110; AKAP110; CT82; FSP95; HEL159; PRKA3; SOB1
External IDs	OMIM: 604689 MGI: 1341149 HomoloGene: 4688 GeneCards: AKAP3 Gene
Gene ontology Molecular function • protein binding • protein kinase A binding Cellular component • acrosomal vesicle • nucleus • cytoplasm • sperm fibrous sheath • sperm principal piece Biological process • movement of cell or subcellular component • transmembrane receptor protein serine/threonine kinase signaling pathway • single fertilization • acrosome reaction • protein localization • regulation of protein kinase A signaling Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	10566	11642
Ensembl	ENSG00000111254	ENSMUSG00000030344
UniProt	O75969	O88987
RefSeq (mRNA)	NM_001278309	NM_009650
RefSeq (protein)	NP_001265238	NP_033780
Location (UCSC)	Chr 12: 4.62 – 4.65 Mb	Chr 6: 126.85 – 126.87 Mb
PubMed search

A-kinase anchor protein 3 is an enzyme that in humans is encoded by the AKAP3 gene.^[1][2][3]

Function

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family, and is expressed in testis only. The encoded protein contains an RII-binding domain, and is predicted to participate in protein-protein interactions with the R-subunit of the PKA. This protein is localized to the ribs of the fibrous sheath in the principal piece of the sperm tail. It may function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.^[3]

Interactions

AKAP3 has been shown to interact with:

• AKAP4^[4]
• GNA13,^[5] and
• PRKAR2A.^[2][6]

References

1. Lefevre A, Duquenne C, Rousseau-Merck MF, Rogier E, Finaz C (May 1999). "Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition". Biochemical and Biophysical Research Communications 259 (1): 60–6. doi:10.1006/bbrc.1999.0728. PMID 10334916.
2. Vijayaraghavan S, Liberty GA, Mohan J, Winfrey VP, Olson GE, Carr DW (May 1999). "Isolation and molecular characterization of AKAP110, a novel, sperm-specific protein kinase A-anchoring protein". Molecular Endocrinology 13 (5): 705–17. doi:10.1210/mend.13.5.0278. PMID 10319321.
3. "Entrez Gene: AKAP3 A kinase (PRKA) anchor protein 3".
4. Brown PR, Miki K, Harper DB, Eddy EM (Jun 2003). "A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the sperm flagellum". Biology of Reproduction 68 (6): 2241–8. doi:10.1095/biolreprod.102.013466. PMID 12606363.
5. Niu J, Vaiskunaite R, Suzuki N, Kozasa T, Carr DW, Dulin N, Voyno-Yasenetskaya TA (Oct 2001). "Interaction of heterotrimeric G13 protein with an A-kinase-anchoring protein 110 (AKAP110) mediates cAMP-independent PKA activation". Current Biology 11 (21): 1686–90. doi:10.1016/S0960-9822(01)00530-9. PMID 11696326.
6. Carr DW, Fujita A, Stentz CL, Liberty GA, Olson GE, Narumiya S (May 2001). "Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA". The Journal of Biological Chemistry 276 (20): 17332–8. doi:10.1074/jbc.M011252200. PMID 11278869.

Further reading

• Lester LB, Scott JD (1997). "Anchoring and scaffold proteins for kinases and phosphatases". Recent Progress in Hormone Research 52: 409–29; discussion 429–30. PMID 9238861.
• Michel JJ, Scott JD (2002). "AKAP mediated signal transduction". Annual Review of Pharmacology and Toxicology 42: 235–57. doi:10.1146/annurev.pharmtox.42.083101.135801. PMID 11807172.
• Mandal A, Naaby-Hansen S, Wolkowicz MJ, Klotz K, Shetty J, Retief JD, Coonrod SA, Kinter M, Sherman N, Cesar F, Flickinger CJ, Herr JC (Nov 1999). "FSP95, a testis-specific 95-kilodalton fibrous sheath antigen that undergoes tyrosine phosphorylation in capacitated human spermatozoa". Biology of Reproduction 61 (5): 1184–97. doi:10.1095/biolreprod61.5.1184. PMID 10529264.
• Carr DW, Fujita A, Stentz CL, Liberty GA, Olson GE, Narumiya S (May 2001). "Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA". The Journal of Biological Chemistry 276 (20): 17332–8. doi:10.1074/jbc.M011252200. PMID 11278869.
• Niu J, Vaiskunaite R, Suzuki N, Kozasa T, Carr DW, Dulin N, Voyno-Yasenetskaya TA (Oct 2001). "Interaction of heterotrimeric G13 protein with an A-kinase-anchoring protein 110 (AKAP110) mediates cAMP-independent PKA activation". Current Biology 11 (21): 1686–90. doi:10.1016/S0960-9822(01)00530-9. PMID 11696326.
• Ficarro S, Chertihin O, Westbrook VA, White F, Jayes F, Kalab P, Marto JA, Shabanowitz J, Herr JC, Hunt DF, Visconti PE (Mar 2003). "Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation". The Journal of Biological Chemistry 278 (13): 11579–89. doi:10.1074/jbc.M202325200. PMID 12509440.
• Brown PR, Miki K, Harper DB, Eddy EM (Jun 2003). "A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the sperm flagellum". Biology of Reproduction 68 (6): 2241–8. doi:10.1095/biolreprod.102.013466. PMID 12606363.
• Hasegawa K, Ono T, Matsushita H, Shimono M, Noguchi Y, Mizutani Y, Kodama J, Kudo T, Nakayama E (Jan 2004). "A-kinase anchoring protein 3 messenger RNA expression in ovarian cancer and its implication on prognosis". International Journal of Cancer. Journal International Du Cancer 108 (1): 86–90. doi:10.1002/ijc.11565. PMID 14618620.
• Lea IA, Widgren EE, O'Rand MG (Jul 2004). "Association of sperm protein 17 with A-kinase anchoring protein 3 in flagella". Reproductive Biology and Endocrinology 2: 57. doi:10.1186/1477-7827-2-57. PMC 484205. PMID 15257753.
• Sharma S, Qian F, Keitz B, Driscoll D, Scanlan MJ, Skipper J, Rodabaugh K, Lele S, Old LJ, Odunsi K (Oct 2005). "A-kinase anchoring protein 3 messenger RNA expression correlates with poor prognosis in epithelial ovarian cancer". Gynecologic Oncology 99 (1): 183–8. doi:10.1016/j.ygyno.2005.06.006. PMID 16005946.
• Lefièvre L, Chen Y, Conner SJ, Scott JL, Publicover SJ, Ford WC, Barratt CL (Sep 2007). "Human spermatozoa contain multiple targets for protein S-nitrosylation: an alternative mechanism of the modulation of sperm function by nitric oxide?". Proteomics 7 (17): 3066–84. doi:10.1002/pmic.200700254. PMC 2777308. PMID 17683036.