Annexin A2

Annexin A2
PDB rendering based on 1w7b.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1W7B​, 1XJL​, 2HYU​, 2HYV​, 2HYW​, 4DRW​, 4FTG​, 4HRH​
Identifiers
Symbols	ANXA2 ; ANX2; ANX2L4; CAL1H; HEL-S-270; LIP2; LPC2; LPC2D; P36; PAP-IV
External IDs	OMIM: 151740 MGI: 88246 HomoloGene: 20857 ChEMBL: 1764938 GeneCards: ANXA2 Gene
Gene ontology Molecular function • protease binding • calcium ion binding • protein binding • calcium-dependent phospholipid binding • phosphatidylinositol-4,5-bisphosphate binding • cytoskeletal protein binding • Rab GTPase binding • phospholipase A2 inhibitor activity • receptor activator activity • S100 protein binding • poly(A) RNA binding • calcium-dependent protein binding Cellular component • ruffle • basement membrane • extracellular space • nucleus • lysosomal membrane • endosome • early endosome • lipid particle • plasma membrane • cell cortex • cell surface • membrane • basolateral plasma membrane • extrinsic component of plasma membrane • midbody • extracellular matrix • late endosome membrane • vesicle • myelin sheath adaxonal region • sarcolemma • melanosome • Schmidt-Lanterman incisure • macropinosome • membrane raft • perinuclear region of cytoplasm • extracellular exosome • PCSK9-AnxA2 complex Biological process • angiogenesis • membrane raft assembly • positive regulation of protein phosphorylation • negative regulation of receptor internalization • membrane budding • body fluid secretion • collagen fibril organization • positive regulation of vesicle fusion • negative regulation of low-density lipoprotein particle receptor catabolic process • osteoclast development • fibrinolysis • negative regulation of catalytic activity • positive regulation of fibroblast proliferation • positive regulation of binding • protein heterotetramerization • protein targeting to plasma membrane • negative regulation of receptor binding • positive regulation of receptor activity Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	302	12306
Ensembl	ENSG00000182718	ENSMUSG00000032231
UniProt	P07355	P07356
RefSeq (mRNA)	NM_001002857	NM_007585
RefSeq (protein)	NP_001002857	NP_031611
Location (UCSC)	Chr 15: 60.35 – 60.4 Mb	Chr 9: 69.45 – 69.49 Mb
PubMed search

Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene.^[1]

Annexin 2 is involved in diverse cellular processes such as cell motility (especially that of the epithelial cells), linkage of membrane-associated protein complexes to the actin cytoskeleton, endocytosis, fibrinolysis, ion channel formation, and cell matrix interactions. It is a calcium-dependent phospholipid-binding protein whose function is to help organize exocytosis of intracellular proteins to the extracellular domain. Annexin II is a pleiotropic protein meaning that its function is dependent on place and time in the body.

Gene

The ANXA2 gene, located at 15q22.2, has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.^[2]

Function

This protein is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.^[2]

Annexin A2 has been proposed to function inside the cell in sorting of endosomes and outside the cell in anticoagulant reactions.

Interactions

Annexin A2 has been shown to interact with Prohibitin,^[3] CEACAM1,^[4] S100A10,^[5][6] PCNA^[7] and complement Factor H ^[8]

See also

• Annexin

References

1. Takahashi S, Reddy SV, Chirgwin JM, Devlin R, Haipek C, Anderson J, Roodman GD (Nov 1994). "Cloning and identification of annexin II as an autocrine/paracrine factor that increases osteoclast formation and bone resorption". The Journal of Biological Chemistry 269 (46): 28696–701. PMID 7961821.
2. "Entrez Gene: ANXA2 annexin A2".
3. Bacher S, Achatz G, Schmitz ML, Lamers MC (Dec 2002). "Prohibitin and prohibitone are contained in high-molecular weight complexes and interact with alpha-actinin and annexin A2". Biochimie 84 (12): 1207–20. doi:10.1016/S0300-9084(02)00027-5. PMID 12628297.
4. Kirshner J, Schumann D, Shively JE (Dec 2003). "CEACAM1, a cell-cell adhesion molecule, directly associates with annexin II in a three-dimensional model of mammary morphogenesis". The Journal of Biological Chemistry 278 (50): 50338–45. doi:10.1074/jbc.M309115200. PMID 14522961.
5. Réty S, Sopkova J, Renouard M, Osterloh D, Gerke V, Tabaries S, Russo-Marie F, Lewit-Bentley A (Jan 1999). "The crystal structure of a complex of p11 with the annexin II N-terminal peptide". Nature Structural Biology 6 (1): 89–95. doi:10.1038/4965. PMID 9886297.
6. He KL, Deora AB, Xiong H, Ling Q, Weksler BB, Niesvizky R, Hajjar KA (Jul 2008). "Endothelial cell annexin A2 regulates polyubiquitination and degradation of its binding partner S100A10/p11". The Journal of Biological Chemistry 283 (28): 19192–200. doi:10.1074/jbc.M800100200. PMC 2443646. PMID 18434302.
7. Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (Oct 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". The Journal of Biological Chemistry 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929.
8. Leffler J, Herbert AP, Norström E, Schmidt CQ, Barlow PN, Blom AM, Martin M (Feb 2010). "Annexin-II, DNA, and histones serve as factor H ligands on the surface of apoptotic cells". The Journal of Biological Chemistry 285 (6): 3766–76. doi:10.1074/jbc.M109.045427. PMC 2823518. PMID 19951950.

Further reading

• Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate plasminogen receptors". Frontiers in Bioscience 10 (1-3): 300–25. doi:10.2741/1529. PMID 15574370.
• Babiychuk EB, Draeger A (Jun 2006). "Regulation of ecto-5'-nucleotidase activity via Ca2+-dependent, annexin 2-mediated membrane rearrangement?". Biochemical Society Transactions 34 (Pt 3): 374–6. doi:10.1042/BST0340374. PMID 16709165.
• Bohn E, Gerke V, Kresse H, Löffler BM, Kunze H (Jan 1992). "Annexin II inhibits calcium-dependent phospholipase A1 and lysophospholipase but not triacyl glycerol lipase activities of rat liver hepatic lipase". FEBS Letters 296 (3): 237–40. doi:10.1016/0014-5793(92)80294-Q. PMID 1531641.
• Dawson SJ, White LA (May 1992). "Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin". The Journal of Infection 24 (3): 317–20. doi:10.1016/S0163-4453(05)80037-4. PMID 1602151.
• Jindal HK, Chaney WG, Anderson CW, Davis RG, Vishwanatha JK (Mar 1991). "The protein-tyrosine kinase substrate, calpactin I heavy chain (p36), is part of the primer recognition protein complex that interacts with DNA polymerase alpha". The Journal of Biological Chemistry 266 (8): 5169–76. PMID 1825830.
• Filipek A, Gerke V, Weber K, Kuźnicki J (Feb 1991). "Characterization of the cell-cycle-regulated protein calcyclin from Ehrlich ascites tumor cells. Identification of two binding proteins obtained by Ca2(+)-dependent affinity chromatography". European Journal of Biochemistry / FEBS 195 (3): 795–800. doi:10.1111/j.1432-1033.1991.tb15768.x. PMID 1999197.
• Becker T, Weber K, Johnsson N (Dec 1990). "Protein-protein recognition via short amphiphilic helices; a mutational analysis of the binding site of annexin II for p11". The EMBO Journal 9 (13): 4207–13. PMC 552202. PMID 2148288.
• Spano F, Raugei G, Palla E, Colella C, Melli M (Nov 1990). "Characterization of the human lipocortin-2-encoding multigene family: its structure suggests the existence of a short amino acid unit undergoing duplication". Gene 95 (2): 243–51. doi:10.1016/0378-1119(90)90367-Z. PMID 2174397.
• Johnsson N, Johnsson K, Weber K (Aug 1988). "A discontinuous epitope on p36, the major substrate of src tyrosine-protein-kinase, brings the phosphorylation site into the neighbourhood of a consensus sequence for Ca2+/lipid-binding proteins". FEBS Letters 236 (1): 201–4. doi:10.1016/0014-5793(88)80314-4. PMID 2456953.
• Gould KL, Woodgett JR, Isacke CM, Hunter T (Jul 1986). "The protein-tyrosine kinase substrate p36 is also a substrate for protein kinase C in vitro and in vivo". Molecular and Cellular Biology 6 (7): 2738–44. doi:10.1128/mcb.6.7.2738. PMC 367834. PMID 2946940.
• Huebner K, Cannizzaro LA, Frey AZ, Hecht BK, Hecht F, Croce CM, Wallner BP (May 1988). "Chromosomal localization of the human genes for lipocortin I and lipocortin II". Oncogene Research 2 (4): 299–310. PMID 2969496.
• Huang KS, Wallner BP, Mattaliano RJ, Tizard R, Burne C, Frey A, Hession C, McGray P, Sinclair LK, Chow EP (Jul 1986). "Two human 35 kd inhibitors of phospholipase A2 are related to substrates of pp60v-src and of the epidermal growth factor receptor/kinase". Cell 46 (2): 191–9. doi:10.1016/0092-8674(86)90736-1. PMID 3013422.
• Buday L, Egan SE, Rodriguez Viciana P, Cantrell DA, Downward J (Mar 1994). "A complex of Grb2 adaptor protein, Sos exchange factor, and a 36-kDa membrane-bound tyrosine phosphoprotein is implicated in ras activation in T cells". The Journal of Biological Chemistry 269 (12): 9019–23. PMID 7510700.
• Chung CY, Erickson HP (Jul 1994). "Cell surface annexin II is a high affinity receptor for the alternatively spliced segment of tenascin-C". The Journal of Cell Biology 126 (2): 539–48. doi:10.1083/jcb.126.2.539. PMC 2200039. PMID 7518469.
• Kato S, Sekine S, Oh SW, Kim NS, Umezawa Y, Abe N, Yokoyama-Kobayashi M, Aoki T (Dec 1994). "Construction of a human full-length cDNA bank". Gene 150 (2): 243–50. doi:10.1016/0378-1119(94)90433-2. PMID 7821789.
• Richard I, Broux O, Chiannilkulchai N, Fougerousse F, Allamand V, Bourg N, Brenguier L, Devaud C, Pasturaud P, Roudaut C (Oct 1994). "Regional localization of human chromosome 15 loci". Genomics 23 (3): 619–27. doi:10.1006/geno.1994.1550. PMID 7851890.
• Takahashi S, Reddy SV, Chirgwin JM, Devlin R, Haipek C, Anderson J, Roodman GD (Nov 1994). "Cloning and identification of annexin II as an autocrine/paracrine factor that increases osteoclast formation and bone resorption". The Journal of Biological Chemistry 269 (46): 28696–701. PMID 7961821.
• Hyatt SL, Liao L, Chapline C, Jaken S (Feb 1994). "Identification and characterization of alpha-protein kinase C binding proteins in normal and transformed REF52 cells". Biochemistry 33 (5): 1223–8. doi:10.1021/bi00171a023. PMID 8110754.
• Wright JF, Kurosky A, Wasi S (Feb 1994). "An endothelial cell-surface form of annexin II binds human cytomegalovirus". Biochemical and Biophysical Research Communications 198 (3): 983–9. doi:10.1006/bbrc.1994.1140. PMID 8117306.
• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.

External links

• Annexin A2 at the US National Library of Medicine Medical Subject Headings (MeSH)