Cyanophycinase

Cyanophycinase
Identifiers
EC number	3.4.15.6
CAS number	131554-16-0
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Cyanophycinase (EC 3.4.15.6, cyanophycin degrading enzyme, beta-Asp-Arg hydrolysing enzyme, CGPase, CphB, CphE, cyanophycin granule polypeptidase, extracellular CGPase) is an enzyme.^[1][2][3] It catalyses the following chemical reaction

[L-Asp(4-L-Arg)]_n + H₂O $\rightleftharpoons$ [L-Asp(4-L-Arg)]_n-1 + L-Asp(4-L-Arg)

The enzyme is highly specific for the branched polypeptide cyanophycin.

References

1. Obst, M., Krug, A., Luftmann, H. and Steinbüchel, A. (2005). "Degradation of cyanophycin by Sedimentibacter hongkongensis strain KI and Citrobacter amalonaticus strain G isolated from an anaerobic bacterial consortium". Appl. Environ. Microbiol. 71: 3642–3652. doi:10.1128/aem.71.7.3642-3652.2005. PMID 16000772.
2. Obst, M., Oppermann-Sanio, F.B., Luftmann, H. and Steinbüchel, A. (2002). "Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI. The cphE gene from P. anguilliseptica BI encodes a cyanophycin-hydrolyzing enzyme". J. Biol. Chem. 277: 25096–25105. doi:10.1074/jbc.m112267200. PMID 11986309.
3. Richter, R., Hejazi, M., Kraft, R., Ziegler, K. and Lockau, W. (1999). "Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartic acid (cyanophycin): molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme". Eur. J. Biochem. 263: 163–169. doi:10.1046/j.1432-1327.1999.00479.x. PMID 10429200.

External links

• Cyanophycinase at the US National Library of Medicine Medical Subject Headings (MeSH)